Collaborative semantic web browsing with Magpie

Web browsing is often a collaborative activity. Users involved in a joint information gathering exercise will wish to share knowledge about the web pages visited and the contents found. Magpie is a suite of tools supporting the interpretation of web pages and semantically enriched web browsing. By automatically associating an ontology-based semantic layer to web resources, Magpie allows relevant services to be invoked as well as remotely triggered within a standard web browser. In this paper we describe how Magpie trigger services can provide semantic support to collaborative browsing activities

The scope of application of incremental rapid prototyping methods in foundry engineering

Abstract The article presents the scope of application of selected incremental Rapid Prototyping methods in the process of manufacturing casting models, casting moulds and casts. The Rapid Prototyping methods (SL, SLA, FDM, 3DP, JS) are predominantly used for the production of models and model sets for casting moulds. The Rapid Tooling methods, such as: ZCast-3DP, ProMetalRCT and VoxelJet, enable the fabrication of casting moulds in the incremental process. The application of the RP methods in cast production makes it possible to speed up the prototype preparation process. This is particularly vital to elements of complex shapes. The time required for the manufacture of the model, the mould and the cast proper may vary from a few to several dozen hours

Towards Activity Context using Software Sensors

Service-Oriented Computing delivers the promise of configuring and reconfiguring software systems to address user's needs in a dynamic way. Context-aware computing promises to capture the user's needs and hence the requirements they have on systems. The marriage of both can deliver ad-hoc software solutions relevant to the user in the most current fashion. However, here it is a key to gather information on the users' activity (that is what they are doing). Traditionally any context sensing was conducted with hardware sensors. However, software can also play the same role and in some situations will be more useful to sense the activity of the user. Furthermore they can make use of the fact that Service-oriented systems exchange information through standard protocols. In this paper we discuss our proposed approach to sense the activity of the user making use of software

Feasibility of diffusion tensor imaging (DTI) with fibre tractography of the normal female pelvic floor

To prospectively determine the feasibility of diffusion tensor imaging (DTI) with fibre tractography as a tool for the three-dimensional (3D) visualisation of normal pelvic floor anatomy. Five young female nulliparous subjects (mean age 28 ± 3 years) underwent DTI at 3.0T. Two-dimensional diffusion-weighted axial spin-echo echo-planar (SP-EPI) pulse sequence of the pelvic floor was performed, with additional T2-TSE multiplanar sequences for anatomical reference. Fibre tractography for visualisation of predefined pelvic floor and pelvic wall muscles was performed offline by two observers, applying a consensus method. Three eigenvalues (λ1, λ2, λ3), fractional anisotropy (FA) and mean diffusivity (MD) were calculated from the fibre trajectories. In all subjects fibre tractography resulted in a satisfactory anatomical representation of the pubovisceral muscle, perineal body, anal - and urethral sphincter complex and internal obturator muscle. Mean FA values ranged from 0.23 ± 0.02 to 0.30 ± 0.04, MD values from 1.30 ± 0.08 to 1.73 ± 0.12 × 10(-)³ mm²/s. Muscular structures in the superficial layer of the pelvic floor could not be satisfactorily identified. This study demonstrates the feasibility of visualising the complex three-dimensional pelvic floor architecture using 3T-DTI with fibre tractography. DTI of the deep female pelvic floor may provide new insights into pelvic floor disorder

Harnessing content and context for enhanced decision making

In a time in which a significant amount of interpersonal interactions take place online, one must enquire to which extent are these milieus suitable for supporting the complexity of our communication. This is especially important in more sensitive domains, such as the one of Online Dispute Resolution, in which inefficient communication environments may result in misunderstandings, poor decisions or the escalation of the conflict. The conflict manager, in particular, may find his skills severely diminished, namely in what concerns the accurate perception of the state of the parties. In this paper the development of a rich communication framework is detailed that conveys contextual information about their users, harnessed from the transparent analysis of their behaviour while communicating. Using it, the conflict manager may not only better perceive the conflict and how it affects each party but also take better contextualized decisions, closer to the ones taken in face-to-face settings.This work is part-funded by ERDF - European Regional Development Fund through the COMPETE Programme (operational programme for competitiveness) and by National Funds through the FCT { Fundação para a Ciência e a Tecnologia (Portuguese Foundation for Science and Technology) within project FCOMP-01-0124-FEDER-028980 (PTDC/EEI-SII/1386/2012) and project PEst- OE/EEI/UI0752/2014

Structure of the Full-Length Major Pilin from Streptococcus pneumoniae: Implications for Isopeptide Bond Formation in Gram-Positive Bacterial Pili

The surface of the pneumococcal cell is adorned with virulence factors including pili. The major pilin RrgB, which forms the pilus shaft on pathogenic Streptococcus pneumoniae, comprises four immunoglobulin (Ig)-like domains, each with a common CnaB topology. The three C-terminal domains are each stabilized by internal Lys-Asn isopeptide bonds, formed autocatalytically with the aid of an essential Glu residue. The structure and orientation of the crucial N-terminal domain, which provides the covalent linkage to the next pilin subunit in the shaft, however, remain incompletely characterised. We report the crystal structure of full length RrgB, solved by X-ray crystallography at 2.8 Å resolution. The N-terminal (D1) domain makes few contacts with the rest of the RrgB structure, and has higher B-factors. This may explain why D1 is readily lost by proteolysis, as are the N-terminal domains of many major pilins. D1 is also found to have a triad of Lys, Asn and Glu residues in the same topological positions as in the other domains, yet mass spectrometry and the crystal structure show that no internal isopeptide bond is formed. We show that this is because β-strand G of D1, which carries the Asn residue, diverges from β-strand A, carrying the Lys residue, such that these residues are too far apart for bond formation. Strand G also carries the YPKN motif that provides the essential Lys residue for the sortase-mediated intermolecular linkages along the pilus shaft. Interaction with the sortase and formation of the intermolecular linkage could result in a change in the orientation of this strand, explaining why isopeptide bond formation in the N-terminal domains of some major pilins appears to take place only upon assembly of the pili