Animal Transport: Developing optimum animal handling procedures and effective transport strategies in the food production chain to improve animal welfare and food quality

End of project reportA series of studies were performed to investigate the effect of transport on liveweight, physiological and haematological responses of cattle. The first study was carried out over a 6 week period in the Spring of 2004. Eighty-four continental x bulls (mean weight (s.d.) 367 (35) kg), naïve to transport, were randomly assigned to one of six journey (J) times of 0, 6, 9, 12, 18 and 24h transport at a stocking density of 1.02m2/bull. Blood samples were collected by jugular venipuncture before, immediately after and at 1, 2, 4, 6, 8, 12 and 24h and bulls were weighed before, immediately after, and at 4, 12 and 24h. Bulls travelling for 6h (280 km), 9h (435 km), 12h (582 km), 18h (902 km) and 24h (1192 km) lost 4.7, 4.5, 5.7 (P=0.05), 6.6 (P=0.05) and 7.5 (P=0.05) percentage liveweight compared with baseline. During the 24h recovery period liveweight was regained to pre-transport levels. Lymphocyte percentages were lower (P=0.001) and neutrophil percentages were higher (P=0.05) in all animals. Blood protein and creatine kinase, glucose and NEFA concentrations were higher (P=0.05) in the bulls following transport and returned to baseline within 24h. In conclusion, liveweight and some physiological and haematological responses of bulls returned to pre-transport levels within 24h having had access to feed and water. Transport of bulls from 6 – 24hours did not impact negatively on animal welfare

The effect of abrupt weaning of suckler calves on the plasma concentrations of cortisol, catecholamines, leukocyte, acute-phase proteins and in vitro interferon-gamma production

End of project reportThe objective of this study was to examine the effect of abrupt weaning (inclusive of social group disruption and maternal separation) on the physiological mediators of stress and measures of immune function. Thirty-eight male and 38 female continental calves were habituated to handling for two weeks prior to bleeding. Calves were blocked on sex, weight and breed of dam and randomly assigned, within block, to either a control (cows remain with calves) or abruptly weaned group (calves removed from cows). Animals were separated into the respective treatment groups at weaning (0 h). Calves were bled at – 168 h, 6 h (males only), 24 h, 48 h and 168 h post weaning. At each sampling time an observer scored the behavioural reaction of calves to sampling. Blood samples were analysed for cortisol, catecholamine concentrations (not sampled at –168 h) and in vitro interferon-gamma production, neutrophil :lymphocyte ratio and acute phase protein concentrations. All continuous data were analysed using a split-plot ANOVA, except that collected at 6 h, which was analysed using a single factor ANOVA model. The effects of weaning, calf sex and time and respective interactions were described. Disruption of the established social groups at 0 h, increased (p<0.001) the plasma cortisol concentration and neutrophil: lymphocyte ratio and reduced the leukocyte concentration (p<0.001) and the in vitro interferon-gamma response to the mitogen concanavalin-A (p<0.001) and keyhole limpet haemocyanin (p<0.001) for weaned and control animals, when compared with –168h. Plasma adrenaline and noradrenaline concentrations were not affected by group disruption. There was no effect of weaning or sex on calf behavioural reaction to handling during blood sampling. Plasma cortisol and adrenaline concentrations were not affected by weaning or sex. Plasma noradrenaline concentration was influenced by weaning x sex (p<0.05) and time x sex (p<0.05). The response increased for male calves with weaning and increased with each sampling time post weaning. For heifers the response was not affected by weaning and plasma concentrations decreased at 168 h post weaning. There was no effect of weaning or sex on leukocyte concentration. The neutrophils : lymphocyte ration increased post weaning (p<0.01) and was affected by sex (p<0.05). Weaning decreased (p<0.05) the in vitro interferon-gamma response to the antigen KLH. There was a time x weaning x sex (p<0.05) interaction for fibrinogen concentration but no effect of treatment on haptoglobin concentration. Abrupt weaning increased plasma cortisol and nor-adrenaline concentrations, which was accompanied by attenuation of in vitro interferon gamma production to novel mitogen and antigen complexes up to 7 days post weaning.European Union Structural Funds (EAGGF

Physiological and behavioural aspects of housing stress in cattle

End of project reportThe effect of various space allowances on pituitary, adrenal, immune responses and performance was investigated in 72 Holstein x Friesian bulls. Bulls (403 ± 3.5 kg) were blocked by weight and randomly assigned into two groups (familiar, F and unfamiliar, UF) x three (1.2, 2.7 and 4.2 m2 per bull; n = 24 bulls per space allowance) treatments and housed for 83 days in 18 pens (n = 4 per pen). Blood samples were collected on day –1, 0, 3, 14, 36 and 77 with respect to mixing and housing on day 0. The bulls were administered with adrenocorticotrophic hormone (ACTH) on day 3 and corticotrophin-releasing hormone (CRH) on days 14, 36 and 77. The basal cortisol concentrations were not affected (P>0.05) by mixing of familiar and unfamiliar bulls. On day 3, basal cortisol was greater (P0.05) of treatment and treatment x time on ACTH. On day 14, interferon-? production was lower (P0.05) different for those housed at 1.2 m2. Bulls housed at either space allowances had (P<0.05) neutrophilia, lymphopenia, eosinopenia and decreased haemoglobin on day 3 compared with day 0. The liveweight gain from days 0 to 83 was lower (P< 0.05) in bulls housed at 1.2 compared with those at 2.7 and 4.2 m2. Housing bulls at 1.2 m2 space allowance had a detrimental effect on their growth and was associated with an acute rise in plasma cortisol concentration (on day 3) compared with space allowances of 2.7 and 4.2 m2/bull.European Union Structural Funds (EAGGF

Cow serum and colostrum immunoglobulin (IgG1) concentration of five suckler cow breed types and subsequent immune status of their calves

peer-reviewedB. Murphy would like to gratefully acknowledge receipt of a Walsh Fellowship provided by Teagasc.The objective of this study was to determine the effect of cow breed type on (a) cow serum and colostrum immunoglobulin (IgG1) concentrations and (b) subsequent calf serum IgG1 concentration and zinc sulphate turbidity (ZST) units. Five cow breed types were examined: LF (Limousin × Friesian), LLF (Limousin × (Limousin × Friesian)), L (Limousin), C (Charolais) and SLF (Simmental × (Limousin × Friesian)). Three blood samples were taken by jugular venipuncture from the cows at approximately 90, 60 and 30 days pre partum, at parturition and at 15 days or more post partum and from the calves at 48 (40 to 56) h post partum. Prior to suckling a 20 ml sample of colostrum was obtained. Milk yield was estimated using the weigh-suckleweigh technique. The decrease in serum IgG1 concentration in cows between 90 days pre partum and parturition was greater (P < 0.01) for LF cows than all other breed types, except SLF. There was no difference between LLF, L, C and SLF cows. There was no effect of cow breed type on colostrum IgG1 concentration. Milk yield was higher (P < 0.001) for LF cows than all other breed types, while that of SLF was higher than the three remaining breed types, which were similar. Calf serum IgG1 concentration and ZST units were higher (P < 0.01) for the progeny of LF cows than all others except SLF. There was no difference between the progeny of LLF, L, C and SLF cows. Calf serum IgG1 was affected by cow breed type and showed a positive relationship with cow serum IgG1 decreases in late pregnancy

From War Room to Boardroom: An Integrative Approach for Helping Today’s Warfighters Become Tomorrow’s Civilian Leaders

What aspects of military experience can help or hinder transitioning veterans in their communication with others? Using the lens of leadership communications, this systematic review seeks to uncover those behaviors which are most likely to result in positive – or negative – outcomes for the veteran transitioning from military service to civilian life. By increasing awareness of these behaviors and their associated outcomes, it is anticipated that service members will have a more positive transition experience. Leadership communication behaviors were identified by performing a search for available academic studies and associated literature for veteran populations, cataloging the results, and individually reviewing each one to identify either positive or negative outcomes associated with the use of leadership communication behaviors. Behaviors were then ranked by their number of associated citations to determine relative strength. Key findings of this review show initiative as the most cited positive leadership communication behavior and anger as the most cited negative. A listing of the top ten positive and negative behaviors is provided along with a discussion of the results. An abbreviated summary of the results suitable for placement in a brochure, magazine, or website article is also included

Beef Cattle Instance Segmentation Using Fully Convolutional Neural Network

In this paper we present a novel instance segmentation algorithm that extends a fully convolutional network to learn to label objects separately without prediction of regions of interest. We trained the new algorithm on a challenging CCTV recording of beef cattle, as well as benchmark MS COCO and Pascal VOC datasets. Extensive experimentation showed that our approach outperforms the state-of-the-art solutions by up to 8% on our data

Horizontal transmission of Escherichia coli O157:H7 during cattle housing, survival kinetics in feces and water of Escherichia coli O157:H7 and characterisation of E. coli O157:H7 isolates from cattle faeces and a feedlot environment

End of project reportTeagasc acknowledges with gratitude the support of European Union Structural Funds (EAGGF) in financing this research projectEscherichia coli O157:H7 can cause severe illness and in some cases leading to death. Cattle are the main reservoir with transmission to humans occurring through contamination of food or the environment. Improved understanding of the survival and transmission and survival of E. coli O157:H7 on the farm is essential for developing future controls of this pathogen. This study showed that transmission of E. coli O157:H7 can occur rapidly in groups of housed cattle, with contamination of the pens and hides occurring in 24 hrs. The inoculation dose for cattle is lower than previously reported. Ingestion of bacteria from the hide through social grooming is important for pathogen transmission in housed cattle along with faecal contamination of the environment. Sampling hide will improve the estimation of prevalence of E. coli O157:H7 in pens

Calf Health and Immunity.

End of Project ReportsSuckled calves had significantly higher serum IgG 1 concentrations than mart purchased dairy calves. The marked differences in immunoglobulin levels between suckled calves and dairy calves suggest that these calves received either insufficient quality or quantity of colostral immunoglobulins. Factors affecting calf serum Ig concentrations are, Ig concentration in colostrum, colostrum intake, Ig mass, calf age at first feeding, nutrition of the dam, method of ingestion, presence of the dam, age of the dam and the calf. When suckled calves were fed a similar volume of colostrum relative to birth weight (40 ml/kg) and at the same time interval post birth, there was no significant difference across the three suckler herd progeny for IgG1, IgA and IgM and total Ig serum levels at 28 and 56 days of age. However, serum IgG2 levels were significantly lower in the Limousin x beef breed when compared with the Charolais x beef breed suckled calves at 28 days of age. Healthy calves had higher serum immunoglobulins (IgG1) than calves treated for respiratory disease, enteric disease or for both respiratory disease and enteric disease. It is well recognised that immunoglobulins are absorbed from the intestine for only a short period post birth and that efficiency of absorption is dependent on ensuring that the calf receives adequate colostrum in the immediate post-partum period. Low serum IgG1 concentrations are attributable to failures to obtain adequate colostral immunoglobulins in the period immediately following birth. The mean IgA and IgM serum levels of suckled calves in the present study were only slightly higher than dairy calves while IgG1 serum levels were almost approximately twice as high. Feeding colostrum high in Ig results in higher calf serum Ig concentrations at 48h. The low serum Ig levels reported in the present study suggest that dairy calves failed to obtain adequate transfer of colostral immunoglobulins. Calves with a lower immune status are more susceptible to neonatal infection and thus the importance of colostrum in the immediate post partum period cannot be overemphasised. Thus, the identification of calves with low levels of immunity might stimulate calf producers to ensure that calves receive adequate levels of colostral immunoglobulins. The implications of the present findings are that compared with suckled calves, dairy calves are not receiving 1). adequate quantity of colostrum 2). adequate quality of colostrum. 3). Colostrum soon enough post birth 4). or a combination of all of the previous factors. Rearing calves outdoors using calf jackets had no beneficial effect on calf performance. The incidence of respiratory disease was higher in calves reared indoor when compared with calves reared outdoor with and without jackets. There was an increased incidence of diarrhoea in calves reared outdoors irrespective of calf jacket. Lymphocytes from calves with respiratory disease manifest an impaired capability to blast in vitro. Chromium (Cr) supplementation (250 mg/kg dry matter intake) enhanced the blastogenic response in healthy calves, while, calves with respiratory had impaired blastogenic responses. Supplementation with organic Cr (250 mg/kg dry matter intake) for 63 days had no major effect on physiological parameters and had select effects on haematological parameters, namely, the % monocytes. The % monocytes were significantly higher in the standard commercial milk replacer (CMR) (Skim) Cr supplemented calves when compared with the whey based (CMR) + Soya Brand B or whey based CMR + Soya Brand C or whey based enzyme processed soya Brand C + Cr treatment groups.European Union Structural Funds (EAGGF

An outside-inside view of exclusive practice within an inclusive mainstream school

This article is a reflection on a sabbatical experience in a mainstream school where an inclusive ethos underpinned the curriculum and environmental approaches for all children. The period as Acting Head teacher raised some challenges for me in reconciling inclusion for all children and the exclusive nature of some professional and physical spaces available to the community of adults working in the school. It has highlighted some development opportunities for the senior management of the school and its governing body