Bekæmpelse af æbleskurv ved brug af plantens egne forsvarsmekanismer

En oplagt mulighed for at bekæmpe æbleskurv, forårsaget af Venturia inaequalis, er at udnytte plantens egne evner til at forsvare sig gennem induceret resistens. Indledende forsøg med induceret resistens mod æbleskurv er sat i gang i projektet StopScab, som er et samarbejde mellem KVl og DJF. I artiklen beskrives kort hvad induceret resistens er, og hvad det kan anvendes til

New fungicides for apple scab control in organic growing

In the future control of apple scab in organic apple growing could be based on the use of natural fungicides like plant extracts and oils. Current projects are testing this idea

Midler til bekæmpelse af skurv i økologiske æbler

Brochuren præsenterer FØJO II projektet StopScab: Bekæmpelse af æbleskurv. I projektet afprøves potentielle alternative bekæmpelsesmidler (planteekstrakter, olier og biologiske bekæmpelsesorganismer)for deres bekæmpelseseffekt overfor æbleskurv (Venturia inaequalis) i laboratorie, vækstkammer og plantage. Udvalgte midler undersøges nærmere for deres virkemekanismer

Roles of reactive oxygen species in interactions between plants and pathogens

The production of reactive oxygen species (ROS) by the consumption of molecular oxygen during host–pathogen interactions is termed the oxidative burst. The most important ROS are singlet oxygen (1O2), the hydroxyperoxyl radical (HO2·), the superoxide anion (O−2), hydrogen peroxide (H2O2), the hydroxyl radical (OH-) and the closely related reactive nitrogen species, nitric oxide (NO). These ROS are highly reactive, and therefore toxic, and participate in several important processes related to defence and infection. Furthermore, ROS also play important roles in plant biology both as toxic by-products of aerobic metabolism and as key regulators of growth, development and defence pathways. In this review, we will assess the different roles of ROS in host–pathogen interactions with special emphasis on fungal and Oomycete pathogens

Biocontrol Effect of Clonostachys rosea on Fusarium graminearum Infection and Mycotoxin Detoxification in Oat (Avena sativa)

Oat (Avena sativa) is susceptible to Fusarium head blight (FHB). The quality of oat grain is threatened by the accumulation of mycotoxins, particularly the trichothecene deoxynivalenol (DON), which also acts as a virulence factor for the main pathogen Fusarium graminearum. The plant can defend itself, e.g., by DON detoxification by UGT-glycosyltransferases (UTGs) and accumulation of PR-proteins, even though these mechanisms do not deliver effective levels of resistance. We studied the ability of the fungal biocontrol agent (BCA) Clonostachys rosea to reduce FHB and mycotoxin accumulation. Greenhouse trials showed that C. rosea-inoculation of oat spikelets at anthesis 3 days prior to F. graminearum inoculation reduced both the amount of Fusarium DNA (79%) and DON level (80%) in mature oat kernels substantially. DON applied to C. rosea-treated spikelets resulted in higher conversion of DON to DON-3-Glc than in mock treated plants. Moreover, there was a significant enhancement of expression of two oat UGT-glycosyltransferase genes in C. rosea-treated oat. In addition, C. rosea treatment activated expression of genes encoding four PR-proteins and a WRKY23-like transcription factor, suggesting that C. rosea may induce resistance in oat. Thus, C. rosea IK726 has strong potential to be used as a BCA against FHB in oat as it inhibits F. graminearum infection effectively, whilst detoxifying DON mycotoxin rapidly

The barley powdery mildew candidate secreted effector protein CSEP0105 inhibits the chaperone activity of a small heat shock protein

Pathogens secrete effector proteins to establish a successful interaction with their host. Here, we describe two barley (Hordeum vulgare) powdery mildew candidate secreted effector proteins, CSEP0105 and CSEP0162, which contribute to pathogen success and appear to be required during or after haustorial formation. Silencing of either CSEP using host-induced gene silencing significantly reduced the fungal haustorial formation rate. Interestingly, both CSEPs interact with the barley small heat shock proteins, Hsp16.9 and Hsp17.5, in a yeast two-hybrid assay. Small heat shock proteins are known to stabilize several intracellular proteins, including defense-related signaling components, through their chaperone activity. CSEP0105 and CSEP0162 localized to the cytosol and the nucleus of barley epidermal cells, whereas Hsp16.9 and Hsp17.5 are cytosolic. Intriguingly, only those specific CSEPs changed localization and became restricted to the cytosol when coexpressed with Hsp16.9 and Hsp17.5, confirming the CSEP-small heat shock protein interaction. As predicted, Hsp16.9 showed chaperone activity, as it could prevent the aggregation of Escherichia coli proteins during thermal stress. Remarkably, CSEP0105 compromised this activity. These data suggest that CSEP0105 promotes virulence by interfering with the chaperone activity of a barley small heat shock protein essential for defense and stress responses