Processed 454 reads for olfactory epithelium

Processed 454 reads for olfactory epithelium from Salmo salar at the parr stage. Trimmed for quality and adaptors (see paper for detail

Assembly of 454 reads from all tissues

Assembly of 454 reads from brain, pituitary and olfactory epithelium of Salmo salar, at the parr stage

Processed 454 reads for pituitary

454 reads for pituitary from Salmo salar at the parr stage. Trimmed for quality and adaptors (see paper for detail

Horned beetles and horn growth.

<p>(A) Three <i>T. dichotomus</i> males (left) with small increases in body size, associated with more dramatic increases in horn size. Males can be up to 7 cm in length (20 g), with a forked horn protruding anteriorly from the head that can be upto 4.5 cm in length. <i>T. dichotomus</i> females (far right) bear no exaggerated horn (photo: A. Johns). Scale bar  = 1 cm (B) Continuous relationship of head horn size and body size in T. dichotomus. Thorax width is used as a proxy for body-size (for justification see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088364#pone.0088364-Moczek1" target="_blank">[20]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088364#pone.0088364-Emlen7" target="_blank">[74]</a>). (C) Large <i>O. nigriventris</i> males (left) are 10–12 mm in length (25 mg) and exhibit a large thoracic horn that can reach upto 16 mm. Females and small males (right) do not grow exaggerated thoracic horns (photo: D. Emlen). Scale bar  = 5 mm (D) Relationship of body size and thoracic horn length in <i>O. nigriventris</i>. (E) A schematic of development during the period prior to pupation, a critical period in horn growth. Late in the third larval instar the larva stops feeding and undergoes gut purge, and becomes a “pre-pupa”. Once the gut purge is complete, the larva undergoes pupation. Head horn imaginal discs form underneath the head capsule, late in the third larval instar. During the pre-pupal period, these imaginal discs undergo intense growth, with horns becoming clearly visible in the pupa (A. Johns unpublished data).</p

Python script for identifying duplicate pairs in Salmo salar

Python script for identifying duplicate pairs in Salmo sala

Processed 454 reads for brain

454 reads for brain from Salmo salar in the parr stage. Trimmed for quality and adaptors (see paper for detail

Effects of morph-biased and sex-biased expression on Ka/Ks in horned beetles.

<p>Contour plots of the effects of morph-biased expression and sex-biased expression on Ka/Ks in <i>O. taurus</i> vs <i>O. nigriventris</i> (A) and <i>O. taurus</i> vs <i>T. dichotomus</i> (B) comparisons. The contours are predicted from the general linear models given in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088364#pone-0088364-t006" target="_blank">Table 6</a>. Super-imposed (blue dots) are the model adjusted values for sex-biased expression and morph-biased expression. These represent the averaged differences in gene expression between sexes and between horned- and horn-less males. These were previously calculated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0088364#pone.0088364-SnellRood1" target="_blank">[28]</a>. Sex-biased expression, morph-biased expression and Ka/Ks have been log-transformed.</p

Assembly of 454 reads from the brain

Assembly of the 454 reads from the brains of Salmo salar par

Python script for protein prediction and ka/ks calculations

Python script for protein prediction and ka/ks calculation

Isogroup metrics.

<p>Isogroup metrics.</p