Phytochemical and antioxidant investigations of a Clausena anisata hook, a South African medicinal plant

Background: Clausena anisata (Willd). Hook (Rutaceae), also known as Iperepesi in Xhosa language is a medicinal plant widely used by herbalists for the treatment and/or management of several ailments such as chronic cough, tuberculosis and lung ulceration in Eastern Cape, South Africa. With reference to the information gathered in our previous study, we investigated the plant’s phyto-constituents, as well as its inhibitory effects using aqueous and two different organic solvent of extractions in order to justify its folkloric usage.Methods: Antioxidant activity of the plant was screened through 1,1- diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis(3-ethylbenzthiazoline-6- sulfonic acid) (ABTS) diammonium salt, nitric oxide (NO), and ferric reducing power. Total phenols, flavonoids, flavonols, proanthocyanidins, tannins, alkaloids and saponins were investigated using spectroscopic techniques.Results: There were no significant differences in the flavonoid and proanthocyanidins contents between the leaves and bark extracts of C.  anisata respectively, while the total phenolic content of the bark extract of C. anisata was significantly higher than that of the C. anisata leaf. The acetone extracts of both the leaf and bark indicated strong antioxidant activities.Conclusion: The observed activities of the plant extracts could be attributed to the high contents of the phenolics, alkaloids, flavonoids, saponins, proanthocyanidins and tannin. The acetone extracts of the plants have also exhibited strong antioxidant activities in vitro. It has been established scientifically that oxidative stress is linked with several degenerative conditions and diseases; the inhibitory effects of these plant extracts on the free radicals could logically justify the folkloric usage of C. anisata leaf and bark in the Eastern Cape for the treatment of respiratory infection diseases.Key words: Antioxidants, Clausena anisata phytochemical contents, solvent extraction antiradica

The effect of Cnidoscolus aconitifolius on multi-drug resistant micro-organisms

This study was carried out to investigate possible bacteriostatic and bactericidal activities of Cnidoscolus aconitifolius leaf extract on multi-drug resistant micro-organisms. The antimicrobial property of C. aconitifolius leaf extract was carried out on the following multi-drug resistant microorganisms;Escherichia coli, Klebsiella species, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella enterica Gallinarum and Candida albicans. The results from this study show that none of the multi-drug resistant micro-organisms was sensitive to the leaf extract of C. aconitifolius.Key words: Cnidoscolus aconitifolius, bioactivity, multi-drug resistant micro-organisms

Survey of urologists on clients’ demand for screening for prostate cancer in Nigeria

Objective: The aim of this article is to document the experience of urologists on clients’ demand for prostate cancer screening among Nigerians.Materials and Methods: The study is a cross-sectional evaluation of the urologists that attended the Fourteenth Annual Meeting and the Scientific Conference of the Nigerian Association of Urological Surgeons. A structured questionnaire was used to assess the estimated workload of the respondents, with reference to prostate cancer management, the stage proportion at the time of diagnosis, and the proportion of patients requesting for CaP screening. The data was analyzed using the SPSS version 15 statistical software.Results: Twenty-two respondents completed and returned the questionnaire and formed the basis of further analysis. About 76.9% of the consultant urologists were also lecturers in their respective universities. They were all actively practicing and a majority of them, 76.9%, had up to 10 years experience as practicing urologists. The majority of the respondents managed between one and fifteen new patients with CaP every month, with 36.4% of them managing more than 15 new patients on an average every month. About 95.4% of all the patients seen by the respondents were diagnosed with advanced stages of the disease, while the remaining ones were incidental findings. About one half of the respondents attended to clients seeking for advice on CaP ‘often’ or ‘very often,’ while 40.9% attended to them ‘occasionally,’ with a mean of 4.67 clients seeking advice per month. The majority of respondents, 20 (22); 90.9%, were ‘not aware’ of any national guideline on CaP screening.Conclusions: A majority of practitioners affirmed that patients with prostate cancer present late. The mean number of patients requesting for screening for prostate cancer per month, per respondent, for CaP, is still low. It is the authors’ belief that the trend may not change until there is an appropriate effort at health education, to enlighten the populace

Proximate And Mineral Composition Of Two Soups As Prepared In The South-West And South-South Regions Of Nigeria

The proximate and mineral composition of okro and ogbono soups prepared with two different Nigeria traditional (South/West, i.e. Yoruba, and South/South, i.e. Efik/Ibibio) recipes was assessed in this study. The crude protein, fat, Ca and Na nutrient composition (30.53 + 0.15% dry weight, 32.58 + 1.32% dry weight, 1132.42 + 9.26mg/100DM and 1803.95 + 130.47mg/100DM respectively), of okro soup prepared with South/South (S/S) recipe were significantly higher (

Performance of Cochorus olitorius as influenced by soil type and organic manure amendments in Yewa North Local Government Area, Ogun State

An experiment was conducted in Yewa North Local Government Area, Ogun State to evaluate the effect of four soil types (loamy, sand, clay and silt) amended with poultry manure (PM), plant manure (PLM), sheep/goat manure (SGM) and NPK on performance of Cochorus olitorius. It was a factorial experiment in completely randomized design. Pre-treated seeds were planted and watered daily. Agronomic data collected on days after planting were plant height, number of leaves and stem girth. Data were analyzed using generalized linear model of SAS version 9.1. Means were separated by least significant difference at P < 0.05. Results obtained indicated that loamy soil had significantly higher plant height, number of leaves and stem girth than other soil types. Silt had the least values for all the parameters measured. Performance of the soil types were in the order Loam > Sand > Clay > Silt. PLM had significantly higher plant height and stem diameter than PM and NPK but not significantly different from that of SGM. Loamy soil amended with either PLM or SGM performed better for all the parameters measured and therefore recommended in this study.Keywords: Organic manure, plant manure, soil type, Cochorus olitorius.African Journal of Biotechnology Vol. 9(33), pp. 5309-5312, 16 August, 201

Knowledge and use of emergency contraception by medical doctors on internship in a tertiary healthcare facility in Nigeria

Context: Emergency contraception (EC) is widely used to prevent unwanted pregnancy and it is largely adopted in many countries as over the counter drug to improve access.Aims: To determine and compare the correct knowledge, attitude and current use of EC among newly graduated medical doctors (MDs).Settings and Design: A cross.sectional study conducted among 255 newly graduated MDs at the University College Hospital, Ibadan, Nigeria.Materials and Methods: A pretested self.administered questionnaire was used to obtain data from consenting participants.Statistical Analysis Used: Descriptive, bivariate, and multivariable analyses were performed, and statistical significance was set at 0.05. Statistical Package for Social Science version 15.0 (Chicago, IL, USA) software was used.Results: The mean age of the respondents was 27.2 years (standard deviation = 2.1). The commonest indication for emergency contraceptive use mentioned was rape.96.5%. About 70% support EC in Nigeria, while about a quarter (26.9%) routinely counsel women about ECP use. About 21% of respondents currently use EC. Logistic regression analysis revealed significant results for gender [odds ratio (OR) =3.64; 95% confidence interval (CI) OR = 1.31.10.01), religion (OR = 0.26; 95% CI OR =  0.11.0.630) and marital status (OR = 0.19; 95% CI = 0.07.0.56). Conclusion: The correct knowledge and professional disposition toward EC as a form of contraception is low. We recommend that in.service training  should focus more on EC to improve the quality of their knowledge and  attitude towards it.Key words: Emergency contraception, knowledge, Medical Doctor, Nigeri

What scans we will read: imaging instrumentation trends in clinical oncology

Oncological diseases account for a significant portion of the burden on public healthcare systems with associated costs driven primarily by complex and long-lasting therapies. Through the visualization of patient-specific morphology and functional-molecular pathways, cancerous tissue can be detected and characterized non- invasively, so as to provide referring oncologists with essential information to support therapy management decisions. Following the onset of stand-alone anatomical and functional imaging, we witness a push towards integrating molecular image information through various methods, including anato-metabolic imaging (e.g., PET/ CT), advanced MRI, optical or ultrasound imaging. This perspective paper highlights a number of key technological and methodological advances in imaging instrumentation related to anatomical, functional, molecular medicine and hybrid imaging, that is understood as the hardware-based combination of complementary anatomical and molecular imaging. These include novel detector technologies for ionizing radiation used in CT and nuclear medicine imaging, and novel system developments in MRI and optical as well as opto-acoustic imaging. We will also highlight new data processing methods for improved non-invasive tissue characterization. Following a general introduction to the role of imaging in oncology patient management we introduce imaging methods with well-defined clinical applications and potential for clinical translation. For each modality, we report first on the status quo and point to perceived technological and methodological advances in a subsequent status go section. Considering the breadth and dynamics of these developments, this perspective ends with a critical reflection on where the authors, with the majority of them being imaging experts with a background in physics and engineering, believe imaging methods will be in a few years from now. Overall, methodological and technological medical imaging advances are geared towards increased image contrast, the derivation of reproducible quantitative parameters, an increase in volume sensitivity and a reduction in overall examination time. To ensure full translation to the clinic, this progress in technologies and instrumentation is complemented by progress in relevant acquisition and image-processing protocols and improved data analysis. To this end, we should accept diagnostic images as “data”, and – through the wider adoption of advanced analysis, including machine learning approaches and a “big data” concept – move to the next stage of non-invasive tumor phenotyping. The scans we will be reading in 10 years from now will likely be composed of highly diverse multi- dimensional data from multiple sources, which mandate the use of advanced and interactive visualization and analysis platforms powered by Artificial Intelligence (AI) for real-time data handling by cross-specialty clinical experts with a domain knowledge that will need to go beyond that of plain imaging

Twelve-month observational study of children with cancer in 41 countries during the COVID-19 pandemic

Introduction Childhood cancer is a leading cause of death. It is unclear whether the COVID-19 pandemic has impacted childhood cancer mortality. In this study, we aimed to establish all-cause mortality rates for childhood cancers during the COVID-19 pandemic and determine the factors associated with mortality. Methods Prospective cohort study in 109 institutions in 41 countries. Inclusion criteria: children <18 years who were newly diagnosed with or undergoing active treatment for acute lymphoblastic leukaemia, non-Hodgkin's lymphoma, Hodgkin lymphoma, retinoblastoma, Wilms tumour, glioma, osteosarcoma, Ewing sarcoma, rhabdomyosarcoma, medulloblastoma and neuroblastoma. Of 2327 cases, 2118 patients were included in the study. The primary outcome measure was all-cause mortality at 30 days, 90 days and 12 months. Results All-cause mortality was 3.4% (n=71/2084) at 30-day follow-up, 5.7% (n=113/1969) at 90-day follow-up and 13.0% (n=206/1581) at 12-month follow-up. The median time from diagnosis to multidisciplinary team (MDT) plan was longest in low-income countries (7 days, IQR 3-11). Multivariable analysis revealed several factors associated with 12-month mortality, including low-income (OR 6.99 (95% CI 2.49 to 19.68); p<0.001), lower middle income (OR 3.32 (95% CI 1.96 to 5.61); p<0.001) and upper middle income (OR 3.49 (95% CI 2.02 to 6.03); p<0.001) country status and chemotherapy (OR 0.55 (95% CI 0.36 to 0.86); p=0.008) and immunotherapy (OR 0.27 (95% CI 0.08 to 0.91); p=0.035) within 30 days from MDT plan. Multivariable analysis revealed laboratory-confirmed SARS-CoV-2 infection (OR 5.33 (95% CI 1.19 to 23.84); p=0.029) was associated with 30-day mortality. Conclusions Children with cancer are more likely to die within 30 days if infected with SARS-CoV-2. However, timely treatment reduced odds of death. This report provides crucial information to balance the benefits of providing anticancer therapy against the risks of SARS-CoV-2 infection in children with cancer