7 research outputs found
Textbooks History of art culture for art and handicraft of secondary vocational schools: didactic analysis of texts
The thesis "School Textbooks of History of Visual Culture for Secondary Art Schools (Didactic analysis of texts)" is focused on the area of visual culture textbooks for professional secondary art education. The secondary art education in the territory of Czech Republic has a long tradition since the middle of 19th century and art history tuition realized in the school subject History of Visual Culture presents a considerable part of professional training of future artists, artisans and craftsmen. The main aim of the school subject is providing students with a complex and systematically structured knowledge of visual culture evolution, art trends and tendencies from the very beginning until the present days. This knowledge will be essential theoretical background for students' professional activities in the field of visual creation. Powered by TCPDF (www.tcpdf.org
Additional file 2: Figure S3. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome
Characterization of the cellular infiltrate and histomorphologic grading of LSG. (A) Shown are representative examples of grade 1 (G1; <50 periductal lymphocytes); (B,C) grade 2 (G2; >50 periductal lymphocytes): nonsegregated and segregated aggregates (NS-G2 and S-G2, respectively); (D,F) grade 3 (G3; >50 periductal lymphocytes, with GC-like structures). (TIF 58923 kb
Additional file 6: Figure S1. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome
Immunohistochemistry for CD19 and ICAM1 in LSG of pSS and healthy control. (A, E) Infiltrating lymphocytes in LSG of pSS were positively staining for CD19 (200× and 400× magnification); (B, F) glandular epithelial cells from control were negative (200× and 400× magnification); (C, G) infiltrating lymphocytes and adjacent glandular epithelial cells in LSG of pSS were positively staining for ICAM1 (200× and 400× magnification). (D, H) Glandular epithelial cells from control were negative (200× and 400× magnification). (TIF 28780 kb
Additional file 1: Table S1. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome
Primer sequences used in validation of lncRNAs. (DOCX 14 kb
Additional file 7: Figure S2. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome
Immunohistochemistry for TLR9 and CXCR4 in LSG of pSS and healthy controls. (A, E) Infiltrating lymphocytes in LSG of pSS positively stained for TLR9 (200× and 400× magnification); (B, F) glandular epithelial cells from control were negative (200× and 400× magnification); (C, G) infiltrating lymphocytes and adjacent glandular epithelial cells in LSG of pSS were positively stained for CXCR4 (200× and 400× magnification). (D, H) Glandular epithelial cells from control were negative (200× and 400× magnification). (TIF 28419 kb
Additional file 3: Figure S4. of Long non-coding RNA expression profile in minor salivary gland of primary Sjögren’s syndrome
EBER expression in LSG of pSS patients. (A) Significant number of EBER+ cells in representative ectopic lymphoid structure-positive LSG tissue. (B) Double staining for CD3 and CD20 in the same section of LSG tissue. (C) Staining for CD21 in the same section of LSG tissue. (TIF 53011 kb
Polypeptide-Functionalized NaYF<sub>4</sub>:Yb<sup>3+</sup>,Er<sup>3+</sup> Nanoparticles: Red-Emission Biomarkers for High Quality Bioimaging Using a 915 nm Laser
We
prepared poly-l-aspartic acid (PASP) functionalized NaYF<sub>4</sub>:Yb<sup>3+</sup>,Er<sup>3+</sup> upconversion nanoparticles
(UCNP-PASP). These nanoparticles can give red upconversion emission
under excitation at 915 nm, whose wavelength of emission and excitation
is located in the optical window of biological tissue. Dynamic laser
scatting and zeta potentials of UCNP-PASP were used to study their
stabilities in different aqueous solution. To understand the mechanism
of the red emission of UCNP-PASP, photoluminescence spectra of samples
were recorded before and after modification with PASP, poly acrylic
acid (PAA), and poly(ether imide) (PEI) ligands under excitation at
915 and 980 nm, respectively. The cytotoxicity of the UCNP-PASP was
also examined on a A549 cell and KB cell by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium
bromide (MTT) assay. Moreover, the PASP-functionalized UCNP was employed
as a potential biomarker for in vitro and in vivo experiments of upconversion
luminescence imaging