Rosa rugosa as an Invader of Coastal Sand Dunes of Cape Breton Island and Mainland of Nova Scotia

Rosa rugosa is described for the first time as an invasive species associated with coastal sand dunes in Atlantic Canada. Our surveys of 24 beaches on western Cape Breton Island and the mainland of northern Nova Scotia from Cheticamp to Fox Harbour showed that 11 of the dune systems (ca. 45%) were colonized. This was more prevalent in Cape Breton where R. rugosa occurred on 9 of 13 systems, whereas only 2 of 9 mainland systems were colonized. Four dunes (three in Cape Breton) were considered heavily colonized with 0.4 - 8.8% of the dune area with cover of R. rugosa. These beaches had 12 - 42 independent clumps with almost monospecific stands over 90% cover. In general, heavily colonized beaches were found adjacent to communities where extensive domestic planting and hedges of R. rugosa occurred and where escapes onto roadsides had occurred. In most colonized beach systems, rhizomes from clones extended 1 - 5 m to produce younger shoots. The absence of Ammophila breviligulata, Lathyrus maritimus and Myrica pensylvanica, from the interior of many clumps of R. rugosa suggests that native dune communities are being negatively impacted. This exacerbates dune integrity already compromised by impacts of sea level rise

Detection of kinetic change points in piece-wise linear single molecule motion

Single-molecule approaches present a powerful way to obtain detailed kinetic information at the molecular level. However, the identification of small rate changes is often hindered by the considerable noise present in such single-molecule kinetic data. We present a general method to detect such kinetic change points in trajectories of motion of processive single molecules having Gaussian noise, with a minimum number of parameters and without the need of an assumed kinetic model beyond piece-wise linearity of motion. Kinetic change points are detected using a likelihood ratio test in which the probability of no change is compared to the probability of a change occurring, given the experimental noise. A predetermined confidence interval minimizes the occurrence of false detections. Applying the method recursively to all sub-regions of a single molecule trajectory ensures that all kinetic change points are located. The algorithm presented allows rigorous and quantitative determination of kinetic change points in noisy single molecule observations without the need for filtering or binning, which reduce temporal resolution and obscure dynamics. The statistical framework for the approach and implementation details are discussed. The detection power of the algorithm is assessed using simulations with both single kinetic changes and multiple kinetic changes that typically arise in observations of single-molecule DNA-replication reactions. Implementations of the algorithm are provided in ImageJ plugin format written in Java and in the Julia language for numeric computing, with accompanying Jupyter Notebooks to allow reproduction of the analysis presented here

Update on the development of a novel dry cow therapy using a bismuth-based intramammary teat seal in combination with the bacteriocin lacticin 3147

peer-reviewedPublic concerns over the widespread prophylactic use of antibiotics have led to a search for alternatives to dry cow therapy for the prevention of intramammary infections. A popular alternative is to infuse a teat seal at drying-off. The teat seal is a viscous non-antibiotic formulation and when it is infused into the teat canal and the teat sinus it forms an internal seal that provides a physical barrier to invasion by mastitis-causing pathogens. Enhancement of teat seal formulations may be achieved using non-antibiotic additives such as bacteriocins, potent proteins produced by some bacteria that have the ability to kill other microorganisms. This paper traces the history of investigations at Moorepark Research Centre into the efficacy of teat seal plus lacticin 3147, a bacteriocin produced by Lactococcus lactis DPC3147, in the prevention of intramammary infections in dry cows. Indications from on-going investigations are that a dry cow formulation combining the two products has considerable potential as a non-antibiotic prophylactic product

Efficacy of a teat dip containing the bacteriocin lacticin 3147 to eliminate Gram-positive pathogens associated with bovine mastitis

On most dairy farms teat dips are applied to the teats of cows either before or after milking in order to prevent pathogens from gaining access to the mammary gland via the teat canal. In the present experiments, a natural teat dip was developed using a fermentate containing the live bacterium Lactococcus lactis DPC 3251. This bacterium produces lacticin 3147, a two-component lantibiotic which was previously shown to effectively kill Gram-positive mastitis pathogens. Lacticin 3147 activity in the fermentate was retained at 53% of its original level following storage for 3 weeks at 4°C. In the initial experiments in vitro, 105 colony-forming units/ml (cfu/ml) of either Staphylococcus aureus, Streptococcus dysgalactiae or Streptococcus uberis were introduced into the lacticin-containing fermentate. Neither Staph. aureus nor Str. dysgalactiae could be detected after 30 min or 15 min, respectively, while Str. uberis was reduced approximately 100-fold after 15 min. Following these trials, preliminary experiments were performed in vivo on teats of lactating dairy cows. In these experiments, teats were coated with each of the challenge organisms and then dipped with the lacticin-containing fermented teat dip. Following a dip contact time of 10 min, staphylococci were reduced by 80% when compared with the undipped control teat. Streptococcal challenges were reduced by 97% for Str. dysgalactiae and by 90% for Str. uberis. These trials showed that the teat dip is able to reduce mastitis pathogens on the teats of lactating cows

Intramammary infusion of a live culture of Lactococcus lactis for treatment of bovine mastitis: comparison with antibiotic treatment in field trials

A treatment containing a live food-grade organism, Lactococcus lactis DPC3147, was compared with conventional antibiotic therapy for its potential to treat bovine chronic subclinical or clinical mastitis in two separate field trials. Effects on disease symptoms and bacteriology were monitored in response to infusion with the culture in each trial. In the first trial, the live culture treatment was compared with an intramammary antibiotic (n=11 quarters for each treatment). Results from this small trial demonstrated that the live culture had potential to be as effective at eliminating chronic subclinical infections as an antibiotic treatment. By day 12, 7 of the 11 quarters treated with the live culture were pathogen-free compared with 5 of the 11 antibiotic-treated infected quarters. Somatic cell counts (SCC) remained relatively unchanged regardless of treatment: average log SCC pre- and post-treatment in the lactococci-treated group were 6·33±0·41 (day 0) and 6·27±0·43 cells/ml (day 12) and average log SCC pre- and post-treatment in the antibiotic-treated group were 6·34±0·37 and 6·22±0·46 cells/ml on day 0 and on day 12, respectively. In the second trial, the live culture was compared with an intramammary antibiotic for the treatment of naturally occurring clinical mastitis cases (n=25 quarters for each treatment). Following a 14-d experimental period, similar bacteriological responses were observed in 7 out of 25 live culture treated quarters and 9 out of 25 antibiotic-treated quarters. Additionally, 15 of 25 cases treated with the culture and 18 of 25 cases treated with the antibiotic did not exhibit clinical signs of the disease following treatment. The results of these trials suggest that live culture treatment with Lc. lactis DPC3147 may be as efficacious as common antibiotic treatments in some instances

Properties of Low-Lying Heavy-Light Mesons

We present preliminary results for the B meson decay constant and masses of low-lying heavy-light mesons in the static limit. Calculations were performed on the lattice in the quenched approximation using multistate smearing functions generated from a Hamiltonian for a spinless relativistic quark. The 2S--1S and 1P--1S mass splittings are measured. Using the 1P--1S charmonium splitting to set the overall scale, the ground state decay constant, f_B, is 319 +- 11 (stat) MeV.Comment: 8 pages, 9 figures, UCLA/92/TEP/4

Masses and Decay Constants of Heavy-Light Mesons Using the Multistate Smearing Technique

We present results for f_B and masses of low-lying heavy-light mesons. Calculations were performed in the quenched approximation using multistate smearing functions generated from a spinless relativistic quark model Hamiltonian. Beta values range from 5.7 to 6.3, and light quark masses corresponding to pion masses as low as 300 MeV are computed at each value. We use the 1P--1S charmonium splitting to set the overall scale.Comment: 9 pages, 13 figures, and 5 tables as a single 193K compressed and uuencoded Postscript file, FERMILAB--CONF--93/376-

Exclusive semileptonic Bs→KℓνB_s\to K \ell \nu decays on the lattice

Semileptonic $B_s \to K \ell \nu$ decays provide an alternative $b$-decay channel to determine the CKM matrix element $|V_{ub}|$, and to obtain a $R$-ratio to investigate lepton-flavor-universality violations. Results for the CKM matrix element may also shed light on the discrepancies seen between analyses of inclusive or exclusive decays. We calculate the decay form factors using lattice QCD with domain-wall light quarks and a relativistic $b$-quark. We analyze data at three lattice spacings with unitary pion masses down to $268\,\mathrm{MeV}$. Our numerical results are interpolated/extrapolated to physical quark masses and to the continuum to obtain the vector and scalar form factors $f_+(q^2)$ and $f_0(q^2)$ with full error budgets at $q^2$ values spanning the range accessible in our simulations. We provide a possible explanation of tensions found between results for the form factor from different lattice collaborations. Model- and truncation-independent $z$-parameterization fits following a recently proposed Bayesian-inference approach extend our results to the entire allowed kinematic range. Our results can be combined with experimental measurements of $B_s \to D_s$ and $B_s\to K$ semileptonic decays to determine $|V_{ub}|=3.8(6)\times 10^{-3}$. The error is currently dominated by experiment. We compute differential branching fractions and two types of $R$ ratios, the one commonly used as well as a variant better suited to test lepton-flavor universality.Comment: Version accepted and published (Phys. Rev. D 107, 114512) 30 pages, 13 Figures, supplementary data fil

Nonperturbative calculations of form factors for exclusive semileptonic B(s)B_{(s)} decays

Precise theoretical predictions derived from the Standard Model are a key ingredient in searches for new physics in the flavor sector. The large mass and long lifetime of the $b$ quark make processes involving $b$ quarks of particular interest. We use lattice simulations to perform nonperturbative QCD calculations for semileptonic $B_{(s)}$ decays. We present results from our determinations of $B_s\to D_s \ell \nu$ and $B_s\to K \ell \nu$ semileptonic form factors and provide an outlook for our $B\to \pi\ell\nu$ calculation. In addition we discuss the determination of $R$-ratios testing lepton-flavor universality and suggest use of an improved ratio. Our calculations are based on the set of 2+1 flavor domain wall Iwasaki gauge field configurations generated by the RBC-UKQCD collaboration featuring three lattice spacings of $1/a = 1.78$, $2.38$, and $2.79\,\text{GeV}$. Heavy $b$-quarks are simulated using the relativistic heavy quark action.Comment: 6 pages, 3 figures, Proceedings of The 40th International Conference on High Energy Physics, ICHEP-2020; Jul 28-Aug 6, 2020, Prague, Czech Republi