Biochemical characteristics and calcium and PTH levels of patients with high normal and elevated serum 25(OH)D levels in Turkey: DeVIT-TOX survey

Summary Vitamin D intake over the recommended dose is usually associated with high serum 25(OH)D levels and gener ally not associated with symptoms of hypercalcemia. High doses of cholecalciferol need to be avoided to protect against vitamin D toxicity and related complications. Strict adherence to the clinical guidelines for treating vitamin D defciency can ensure safe and efective treatment. Purpose We observed a tendency to use high doses of cholecalciferol for vitamin D defciency treatment or vitamin D supplementation. We aimed to determine the biochemical characteristics of patients with high normal and elevated serum 25(OH)D levels. Methods An online invitation was sent to all tertiary endocrinology clinics in Turkey to complete an online retrospective survey (DeVIT-TOX Survey) for patients diagnosed with high serum 25(OH)D levels (>88 ng/mL) between January 2019 and December 2019. The patients were evaluated according to the presence of signs and symptoms of hypercalcemia and doses of vitamin D intake, evaluated into the following three groups according to their 25(OH)D levels: group 1,>150 ng/ mL; group 2, 149–100 ng/mL; and group 3, 99–88 ng/mL. Results A total of 253 patients were included in the fnal analysis (female/male: 215/38; mean age, 51.5±15.6 years). The average serum 25(OH)D level was 119.9±33 (range, 88–455) ng/mL, and the average serum calcium level was 9.8±0.7 (range, 8.1–13.1) mg/dL. Most (n=201; 75.4%) patients were asymptomatic despite having high serum 25(OH)D and cal cium levels. The serum 25(OH)D level was signifcantly higher in the symptomatic groups than in the asymptomatic groups (138.6±64 ng/mL vs. 117.7±31 ng/mL, p<0.05). The most common cause (73.5%) associated with high serum 25(OH) D levels was the inappropriate prescription of a high dose of oral vitamin D (600.000–1.500.000 IU) for treating vitamin D defciency/insufciency in a short time (1–3 months). The cut-of value of 25 (OH) D level in patients with hypercalcemia was found to be 89 ng/mL [median 116.5 (89–216)]. Conclusions High dose of vitamin D intake is associated with a high serum 25 OH D level, without symptoms of hypercal cemia. Inappropriate prescription of vitamin D is the primary cause for elevated 25(OH) D levels and related hypercalcemia. Hypercalcemia may not be observed in every patient at very high 25(OH) D levels. Adherence to the recommendation of guidelines is essential to ensure safe and efective treatment of vitamin D defciency

Batı Anadolu bölgesi domates üretim alanlarında görülen stolbur hastalığının tohumla taşınıp taşınmadığı ile ilgili bir araştırma

Tomato (Lycopersicum esculantum Mill), is one of the most important vegetable crops in our country. A survey was carried out in tomato production areas, especially in Western Anatolia region, Bursa and çanakkale. Tomato seeds were collected from tomato fields with stolbur disease observed. They were used as a material. Total genomic DNA were isolated from seeds and tomato plants and then amplified by universal primer sets (P1/ P6 and P1/P7) spesific for phytoplasma . the results showed that the role of the seed in spreading of stolbur disease in tomato was not confirmed.Domates (Lycopersicum esculantum Mill), ülkemizde üretimi yapılan sebzeler arasında ilk sıralarda yer almaktadır. Ülkemizde domates üretiminin en çok yapıldığı Batı Anadolu Bölgesi’nde domates stolbur hastalığı’nın yoğun olarak görüldüğü Bursa ve Çanakkale illerinin domates üretimi yapılan ilçe ve köylerinde survey çalışmaları yapılmıştır. Stolbur hastalığının görüldüğü dönemde araziden toplanan stolbur hastalığı belirtisi veren domates bitkilerinden ve sağlıklı bitkilerden alınan domates tohumları materyal olarak kullanılmıştır. PCR yöntemi ile P1/P6, P1/P7 üniversal primer setleri kullanılarak stolbur hastalığının varlığı saptanmaya çalışılmıştır. Çalışmalarımız sonucunda, domates stolbur hastalığının, yoğun olarak görüldüğü bölgeler saptanmış ve domates stolbur hastalığının tohumla taşınmadığı sonucuna varılmıştır

A study on the transmission with the seed of stolbur disease In Western Anatolia tomato areas

Bu çalışma, Ege Üniversitesi Fen Bilimleri Enstitüsünde Hikmet Saygılı'nın danışmanlığında Nilay Özdemır tarafından hazırlanan "Batı Anadolu bölgesi domates üretim alanlarında görülen stolbur hastalığının yaygınlığının belirlenmesi, tanılanması ve taşınma yolları üzerinde çalışmalar" adlı doktora tezine dayanılarak hazırlanmıştır.Domates (Lycopersicum esculantum Mill), ülkemizde üretimi yapılan sebzeler arasında ilk sıralarda yer almaktadır. Ülkemizde domates üretiminin en çok yapıldığı Batı Anadolu Bölgesi’nde domates stolbur hastalığı’nın yoğun olarak görüldüğü Bursa ve Çanakkale illerinin domates üretimi yapılan ilçe ve köylerinde survey çalışmaları yapılmıştır. Stolbur hastalığının görüldüğü dönemde araziden toplanan stolbur hastalığı belirtisi veren domates bitkilerinden ve sağlıklı bitkilerden alınan domates tohumları materyal olarak kullanılmıştır. PCR yöntemi ile P1/P6, P1/P7 üniversal primer setleri kullanılarak stolbur hastalığının varlığı saptanmaya çalışılmıştır. Çalışmalarımız sonucunda, domates stolbur hastalığının, yoğun olarak görüldüğü bölgeler saptanmış ve domates stolbur hastalığının tohumla taşınmadığı sonucuna varılmıştır.Tomato (Lycopersicum esculantum Mill), is one of the most important vegetable crops in our country. A survey was carried out in tomato production areas, especially in Western Anatolia region, Bursa and Çanakkale. Tomato seeds were collected from tomato fields with stolbur disease observed. They were used as a material. Total genomic DNA were isolated from seeds and tomato plants and then amplified by universal primer sets (P1/ P6 and P1/P7) spesific for phytoplasma . The results showed that the role of the seed in spreading of stolbur disease in tomato was not confirmed

POTENTIAL OF BACTERIOPHAGES TO CONTROL BACTERIAL SPECK OF TOMATO (PSEUDOMONAS SYRINGAE pv. TOMATO)

Tomato (Solanum lycopersicum L.), is one of the widely grown vegetable crop throught the world. The bacterium, Pseudomonas syringae pv. tomato (Pst), causes bacterial speck disease on tomatoes. Several control strategies are performed for disease management. As a strategy, bacteriophages, are natural enemies of bacteria and extremely specific to their targeted hosts without affecting any other bacteria. In this study, isolation of Pseudomonas syringae pv. tomato-lysing bacteriophages, potential of bacteriophages as biological control agents and efficacy of phages on other tomato infecting and saprophytic bacteria were investigated. Samples of tomato plants with typical symptoms of bacterial speck disease were collected from 17 tomato fields, 12 greenhouses and four nurseries in Adana and Mersin provinces in the Eastern Mediterranean Region of Turkey. The plaques (inhibition zones) were observed after 36 hours of incubation, and 47 putative bacteriophages were purified. Among the obtained bacteriophages, phage PH 33 isolate was completely (100 %) suppressed the growth of pathogen and increased the germination rate by %14.6, whereas the phage PH 34 was suppressed the pathogen by 65.8 % and increased the germination rate by 21.6 %. These phages (PH 33 and PH 34) had no inhibition effect on the other tomato bacterial diseases and the saprophytic bacterial flora on tomatoes. Both bacteriophages were specific to Pseudomonas syringae pv. tomato. To our knowledge, this is the first detailed study about isolation of bacteriophages against Pst and efficacy of bacteriophages for biocontrol of Pst in Turkey

The Importance of Lower Gastrointestinal Tract Endoscopy Regarding the Preoperative Evaluation of Malignant Adnexal Masses

AbstractObjective: Our aim in this study was to investigate the value of lower gastrointestinal system (GIS) endoscopy regarding the detection of colon invasion and its importance in recognizing primary and secondary ovarian cancers in cases clinically prediagnosed as advanced stage ovarian cancers.Materials and Methods: Records of patients, who were operated due to adnexal mass suspicious for malignancy at our clinic between September 2012 and May 2017, were examined. One hundred thirteen cases of advanced stage (Stage III – IV) malignant adnexal masses were detected.Results: Cases that underwent laparotomy because of a prediagnosis of malignant adnexal mass (mostly ovarian), and had stage III and IV disease, were compared regarding clinical characteristics and foreseeing bowel resection (51 patients had undergone lower GIS endoscopy, 62 had not). Six of the 51 patients, who underwent endoscopy, were diagnosed with colon involvement during endoscopy while 4 other patients were diagnosed intraoperatively. Among the 62 patients, without preoperative endoscopy, 10 patients underwent intraoperative bowel resection. The mean age of the patients with bowel resection was 57.35±13.53y; the mean age of the remaining patients was 55.8±12.54y. Rectosigmoid region was the most common area of resection (17/20). The positive predictive value of colonoscopy for predicting bowel resection was 100%, while the negative predictive value was 91%.Conclusion: Bowel resection is a pivotal component of the surgical approach to advanced stage malignant adnexal masses. The detection of tumor spread in lower GIS endoscopy is very important while planning the surgery, dealing with postoperative stoma problems and emotional issues and during the differential diagnosis of metastatic tumors.</div

F1 Melez Armut Populasyonunun Ateş Yanıklığı Hastalığı Etmeni Erwinia amylovora Karşı Reaksiyonunun Belirlenmesi

In this study, susceptibility levels of different hybrid pear individuals were determined against fire blight disease agent Erwinia amylovora by artificial inoculations. According to the pathogenity tests of 75 different E. amylovora strains, made on apple and pear shoots, highly virulent 7 strains were used in the testing of the hybrids. E. amylovora suspension was prepared with equal mixes of these 7 selected strains. This suspension was inoculated to hybrid plants by shoot injections that carried out twice, in August 2010 and May 2011. According to the evaluations made through necrotic shoot rate, totaly 7495 hybrid seedlings inoculated, and 11,91% of them were “the least susceptible” 3,99% were “less susceptible”, 6,62% were “moderately susceptible”, 18,07% were “highly susceptible”, 59,41% were “the most susceptible”. More “the least susceptible” pear hybrids were obtained from the combinations of resistant Magness cultivar than the other combinations.Ateş Yanıklığı hastalığına dayanıklı armut tiplerinin belirlenmesini amaçlayan bu çalışmada, melez armut bireylerin Ateş Yanıklığı hastalığı etmeni Erwinia amylovora’ya karşı duyarlılık durumu suni inokulasyonla ortaya konmuştur. Bireylerin testlemesinde, 75 farklı E. amylovora izolatı içinden, elma ve armut sürgünlerinde yapılan patojenite testlerine göre virulensi yüksek olan 7 izolat seçilerek kullanılmıştır. Melez bireyler bu izolatların eşit oranda karışımlarından oluşan E. amylovora süspansiyonu ile Ağustos 2010 ve Mayıs 2011 tarihlerinde sürgün injeksiyonuyla iki kez inokule edilmiştir. Sürgün yanıklığı oranına göre yapılan değerlendirmede, testlenen 7495 melez bitkiden, %11,91’u “çok az duyarlı”, %3,99’u “az duyarlı”, %6,62’si “orta derecede duyarlı”, %18,07’si “duyarlı” ve %59,41’i “çok duyarlı” grupta yer almıştır. Dayanıklı Magness çeşidinin ana ebeveyn olarak kullanıldığı kombinasyonlarda “çok az duyarlı” grupta yer alan birey sayısının yüksek olduğu görülmüştür