Programmed cell death and genetic stability in conifer embryogenesis

Somatic embryogenesis, the generation of embryos from somatic cells, is a valuable tool for studying embryology. In addition, somatic embryos can be used for large-scale vegetative propagation, an application of great interest for forestry. A critical event during early embryo differentiation in conifers is the apical basal polarization, which proceeds through the establishment of two embryonic parts: the proliferating embryonal mass and the terminally differentiated suspensor. The development of both parts is strictly coordinated and imbalance causes embryonic defects. The suspensor cells are eliminated by programmed cell death (PCD). In animals, caspase family proteases are the main executioners of PCD. In this work we have used synthetic peptide substrates containing caspase recognition sites and corresponding specific inhibitors to analyse the role of caspase-like activity during early embryo differentiation in Norway spruce (Picea abies L. Karst.). We found that VEIDase is the principal caspase-like activity. This activity is localized specifically in suspensor cells, and its inhibition prevents normal embryo development by blocking the suspensor differentiation. The in vitro VEIDase activity was shown to be highly sensitive to pH, ionic strength, temperature and zinc concentration. In vivo studies with Zinquin, a zinc-specific fluorescent probe, revealed a high accumulation of intracellular free zinc in the embryonal masses and an abrupt decrease in the suspensor. Increased zinc concentration in the culture medium suppresses terminal differentiation and PCD of the suspensor. In accordance, exposure of early embryos to TPEN, a zinc-specific chelator, induces ectopic cell death affecting embryonal masses. This establishes zinc as an important factor affecting cell fate specification during plant embryogenesis. Before somatic embryos can be accepted for clonal propagation it is important to show that the regenerated plants have similar growth to that of seedlings and are genetically uniform. The genetic integrity during zygotic and somatic embryogenesis in Norway spruce and Scots pine (Pinus sylvestris L.) was investigated by comparing the stability of variable nuclear microsatellite loci. The stability varied significantly among families in both species during somatic embryogenesis. Scots pine families showing low genetic stability during establishment of embryogenic cultures had a higher embryogenic potential than those that were genetically more stable. In contrast, embryo development was suppressed in genetically unstable families. The stability of microsatellites was in general higher in zygotic embryos than in somatic embryos. No deviation in growth was observed in somatic embryo plants of Norway spruce carrying mutated microsatellites

The Shareholders’ Derivative-Claim Exception to the Attorney-Client Privilege

The use of an over-parametrized state-space model for system identification has some clear advantages: A single model structure covers the entire class of multivariable systems up to a given order. The over-parametrization also leads to the possibility to choose a numerically stable parametrization. During the parametric optimization the gradient calculations constitute the main computational part of the algorithm. Consequently using more than theminimal number of parameters requiredslows down thealgorithm. However, we show that for any chosen (over)-parametrization it is possible to reduce the gradientcalculations to the minimal amount by constructing the parameter subspace which is orthonormal to the tangent space of the manifold representing equivalent models

EC Maritime Transport Policy and Regulation

When designing robust controllers, H-infinity synthesis is a common tool touse. The controllers that result from these algorithms are typically of very high order, which complicates implementation. However, if a constraint on the maximum order of the controller is set, that is lower than the order of the (augmented) system, the problem becomes nonconvex and it is relatively hard to solve. These problems become very complex, even when the order of the system is low. The approach used in this work is based on formulating the constraint onthe maximum order of the controller as a polynomial (or rational) equation.This equality constraint is added to the optimization problem of minimizingan upper bound on the H-innity norm of the closed loop system subjectto linear matrix inequality (LMI) constraints. The problem is then solvedby reformulating it as a partially augmented Lagrangian problem where theequality constraint is put into the objective function, but where the LMIsare kept as constraints. The proposed method is evaluated together with two well-known methodsfrom the literature. The results indicate that the proposed method hascomparable performance in most cases, especially if the synthesized con-troller has many parameters, which is the case if the system to be controlledhas many input and output signals

Sometimes the Silence Can Be like the Thunder: Access to Pharmaceutical Data at the FDA

Those committed to the free exchange of scientific information have long complained about various restrictions on access to the FDA\u27s pharmaceutical data and the resultant restrictions on open discourse. A review of open-government procedures and litigation at the FDA demonstrates that the need for transparency at the agency extend well beyond the reach of any clinical trial registry

Microsoft and the European Union Face Off Over Internet Privacy Concerns

Amidst what appears to be a multi-faceted attack by the European Union on Microsoft, the newest angle is the European Commission\u27s announcement last month that it was considering a formal investigation of Microsoft\u27s .Net Passport data processing system for possible violations of the European Union Data Privacy Directive. This iBrief explores the European Data Privacy Directive and seeks to explain why the European Commission believes .Net Passport may be in violation of its privacy policies and a case for further investigation

Dual Control of an Integrator with Unknown Gain

The dual control law for an integrator with constant but unknown gain is computed. Numerical problems associated with the solution of the Bellman equation are reviewed. Properties of the dual control law are discussed. A representation which makes it easy to compare dual control with certainty equivalence and cautious control is also introduced

Synthesis of titanium-oxide nanoparticles with size and stoichiometry control

Ti-O nanoparticles have been synthesized via hollow cathode sputtering in an Ar-O2 atmosphere using high power pulsing. It is shown that the stoichiometry and the size of the nanoparticles can be varied independently, the former through controlling the O2 gas flow and the latter by the independent biasing of two separate anodes in the growth zone. Nanoparticles with diameters in the range of 25 to 75 nm, and with different Ti-O compositions and crystalline phases, have been synthesized.Comment: 15 pages, 6 figure

The role of relationships in start-up development

Relationships are important in the business start-up phase for a variety of reasons. Internal relationships can support knowledge exchange that determines the business model development and external relationships can facilitate a wide range of opportunities, support and insights. The paper explains the key relationships experienced by the founding team of a recently formed Swedish digital trading platform. Data were gathered through a self-reporting diary approach based on the Critical Incident Technique format and texts were analysed by the Pertex text-analytic software. The findings explain how important relationships were formed during the initial start-up period targeting international expansion. Interaction with early adopters enabled a rapid evolution of the business platform with the aim to build a community of users to support development