Sorting nexin-27 regulates AMPA receptor trafficking through the synaptic adhesion protein LRFN2

The endosome-associated cargo adaptor sorting nexin-27 (SNX27) is linked to various neuropathologies through sorting of integral proteins to the synaptic surface, most notably AMPA receptors. To provide a broader view of SNX27-associated pathologies, we performed proteomics in rat primary neurons to identify SNX27-dependent cargoes, and identified proteins linked to excitotoxicity, epilepsy, intellectual disabilities, and working memory deficits. Focusing on the synaptic adhesion molecule LRFN2, we established that SNX27 binds to LRFN2 and regulates its endosomal sorting. Furthermore, LRFN2 associates with AMPA receptors and knockdown of LRFN2 results in decreased surface AMPA receptor expression, reduced synaptic activity, and attenuated hippocampal long-term potentiation. Overall, our study provides an additional mechanism by which SNX27 can control AMPA receptor-mediated synaptic transmission and plasticity indirectly through the sorting of LRFN2 and offers molecular insight into the perturbed function of SNX27 and LRFN2 in a range of neurological conditions

Efficiency of ambient vibration HVSR investigations in soil engineering studies : backfill study in the Algiers (Algeria) harbor container terminal

This paper deals with the contribution of the ambient vibration horizontal-to-vertical spectral ratio (HVSR) method in soil engineering studies, particularly in backfill compactness assessment. The study is based on 60 ambient vibration recordings performed in 2015 at the container terminal of Algiers harbor, subjected a year before to a geotechnical study based on 23 boreholes and 13 cone penetration tests (CPT) for backfill improvement. To highlight the contribution of the HVSR method, the results of the geotechnical and HVSR studies are first analyzed separately and then in combination. The HVSR method provides a compactness zonation map based on peak amplitude variation. Both methods define the same pattern: a southern section where the backfill is more compact, and a northern section where the backfill is less compact. This shows that the HVSR peak amplitudes are sensitive to compactness variations, which may be sufficient for qualitative zonation. In addition, with the combination of the two methods, rough estimations of shear-wave velocity and thickness of the backfill can be retrieved. This study shows that the HVSR method can be a very useful investigative tool in soil engineering studies. When the HVSR method is deployed before any conventional technique, a geotechnical investigation campaign can be significantly optimized. Moreover the combined interpretation brings complementary quantitative soil information

Mediterranean Sea and anthropogenic influences on ambient vibration amplitudes in the low-frequency and high-frequency domains in the Algiers region

Ambient vibrations have been continuously recorded at Dar El Beida, about 20 km from Algiers (Algeria). This data set allows determining that, in the low-frequency domain (<1 Hz), ambient vibration sources are mainly linked to Mediterranean Sea effects, while in the high-frequency domain, they are closely related to anthropogenic activity. Climatic conditions have an influence on the ambient vibration spectral amplitudes in the low-frequency domain, which is not the case in the high-frequency domain. The limit between the low-frequency and high-frequency domain, based on natural versus anthropogenic activity, is not clear cut and lies between 1.25 and 1.50 Hz. Variations of H/V peak amplitudes in the low-frequency domain are clearly linked to the climatic conditions. In the high-frequency domain, H/V peaks are not related to climatic conditions and cannot be clearly related to anthropogenic source changes

Heavier Damages without Site Effects and Site Effects with Lighter Damages: Boumerdes City (Algeria) after the May 2003 Earthquake

International audienc

Smooth bumps in H/V curves over a broad area from single-station ambient noise recordings are meaningful and reveal the importance of Q in array processing: The Boumerdes (Algeria) case

International audienceSingle-station H/V curves from ambient noise recordings in Boumerdes (Algeria) show smooth bumps around 1 and 3 Hz. A complementary microtremor study, based on two 34 and 134-meter aperture arrays, evidences that these bumps are indeed real peaks produced by two strong VS contrasts at 37 and 118 meters depth, strongly smoothed by very high S-wave attenuation in the two sedimentary layers. These two H/V bumps, observed over a broad area, are meaningful and reveal the importance of Q in S-wave velocity modeling from microtremor array data processing. It also appears that Tertiary rocks should be, at least in some cases, taken into account, together with the Quaternary sediments, to explain single-station H/V frequency peaks, and therefore that considering only the first 30 m of soil for VS amplification evaluation, as usually recommended, sometimes leads to flaky results by artificially eliminating non-explained low-frequency peaks from the analysis