Keck IR Spectroscopy of WZ Sge: Detection of Molecular Emission from the Accretion Disk

Time-resolved IR spectroscopy of WZ Sge was obtained using NIRSPEC on Keck II. We detect CO and H$_{\rm 2}$ emission from the accretion disk placing WZ Sge into a rarefied class of astronomical objects including YSOs and high luminosity early-type stars. During the eclipse phase, the molecular emission greatly weakens but no firm evidence for the secondary star is seen allowing new limits on its luminosity to be determined. The detection of molecular emission provides physical properties within the outer disk of T=3000K and N$_H$$>10^{10}$ cm$^{-3}$. Such a cool, dense region, not associated with areas of H I and He I emission, provides the first observational confirmation of predictions made by accretion disk models.Comment: 10 pages, 3 figures. Accepted for publication in ApJ Letter

Finite pseudo orbit expansions for spectral quantities of quantum graphs

We investigate spectral quantities of quantum graphs by expanding them as sums over pseudo orbits, sets of periodic orbits. Only a finite collection of pseudo orbits which are irreducible and where the total number of bonds is less than or equal to the number of bonds of the graph appear, analogous to a cut off at half the Heisenberg time. The calculation simplifies previous approaches to pseudo orbit expansions on graphs. We formulate coefficients of the characteristic polynomial and derive a secular equation in terms of the irreducible pseudo orbits. From the secular equation, whose roots provide the graph spectrum, the zeta function is derived using the argument principle. The spectral zeta function enables quantities, such as the spectral determinant and vacuum energy, to be obtained directly as finite expansions over the set of short irreducible pseudo orbits.Comment: 23 pages, 4 figures, typos corrected, references added, vacuum energy calculation expande

Spitzer Observations of Black Hole Low-mass X-ray Binaries: Assessing the Non-stellar Infrared Component

We have combined ground-based optical and near-infrared data with Spitzer Space Telescope mid-infrared data for five black hole (BH) soft X-ray transients (SXTs) in order to determine the levels of near- and mid-infrared emission from sources other than the secondary star. Mid-infrared emission from an accretion disk, circumbinary dust, and/or a jet could act as sources of near-infrared contamination, thereby diluting ellipsoidal variations of the secondary star and affecting determined BH mass estimates. Based on optical to mid-infrared spectral energy distribution modeling of the five SXTs along with the prototype, V616 Mon, we detected mid-infrared excesses in half of the systems, and suggest that the excesses detected from these systems arise from non-thermal synchrotron jets rather than circumbinary dust disks

The NuSTAR View of the Seyfert 2 Galaxy NGC 4388

We present analysis of NuSTAR X-ray observations in the 3-79 keV energy band of the Seyfert 2 galaxy NGC 4388, taken in 2013. The broadband sensitivity of NuSTAR, covering the Fe K$\alpha$ line and Compton reflection hump, enables tight constraints to be placed on reflection features in AGN X-ray spectra, thereby providing insight into the geometry of the circumnuclear material. In this observation, we found the X-ray spectrum of NGC 4388 to be well described by a moderately absorbed power law with non-relativistic reflection. We fit the spectrum with phenomenological reflection models and a physical torus model, and find the source to be absorbed by Compton-thin material (N$_{H} = (6.5\pm0.8)\times10^{23}$ cm$^{-2}$) with a very weak Compton reflection hump (R $<$ 0.09) and an exceptionally large Fe K$\alpha$ line (EW $= 368^{+56}_{-53}$ eV) for a source with weak or no reflection. Calculations using a thin-shell approximation for the expected Fe K$\alpha$ EW indicate that an Fe K$\alpha$ line originating from Compton-thin material presents a possible explanation.Comment: 5 pages, 2 figures. Accepted for publication in Ap

Molecular Analysis of N6-Methyladenine Patterns in \u3cem\u3eTetrahymena thermophila\u3c/em\u3e Nuclear DNA

We have cloned two DNA fragments containing 5\u27-GATC-3\u27 sites at which the adenine is methylated in the macronucleus of the ciliate Tetrahymena thermophila. Using these cloned fragments as molecular probes, we analyzed the maintenance of methylation patterns at two partially and two uniformly methylated sites. Our results suggest that a semiconservative copying model for maintenance of methylation is not sufficient to account for the methylation patterns we found during somatic growth of Tetrahymena. Although we detected hemimethylated molecules in macronuclear DNA, they were present in both replicating and nonreplicating DNA. In addition, we observed that a complex methylation pattern including partially methylated sites was maintained during vegetative growth. This required the activity of a methylase capable of recognizing and modifying sites specified by something other than hemimethylation. We suggest that a eucaryotic maintenance methylase may be capable of discriminating between potential methylation sites to ensure the inheritance of methylation patterns

Conjugative transfer frequencies of mef(A)-containing Tn1207.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types

The aim of this study was to examine the gene transfer potential of mef(A)-containing Tn120.3 to macrolide-susceptible Streptococcus pyogenes belonging to different emm types. Using the filter mating technique, Tn1207.3 was transferred by conjugation to 23 macrolide-susceptible recipients representing 11 emm types. PCR analysis confirmed the presence of the mef(A) gene and the comEC junction regions of the Tn1207.3 insertion in resultant transconjugants. Significant variation was found in the transfer frequency of Tn1207.3 to different Strep. pyogenes strains, and this phenomenon may contribute to the differences in mef(A) frequency observed among clinical isolates. Significance and Impact of the Study: The spread of antimicrobial resistance among pathogenic bacteria is an important problem, but the mechanisms of horizontal transfer between strains and species are often poorly understood. For instance, little is known on how macrolide resistance spreads between strains of the human pathogen Strep. pyogenes and why certain strains more commonly display resistance than others. Here, we show that Strep. pyogenes strains vary greatly in their ability to acquire a transposon encoding macrolide resistance by horizontal gene transfer in vitro. These data provide a novel insight into the transfer of antibiotic resistance between bacterial strains and offer an explanation for the differences in the frequency of resistance determinates and resistance seen among clinical isolates. © 2014 The Authors Letters in Applied Microbiology

Development of a Miniature Electrostatic Accelerometer /MESA/ for low g applications Summary report

Design, fabrication, and testing of miniature digital electrostatic accelerometer for low gravity measurements in spac