Prevalence of extreme detergent resistance among the \u3ci\u3eEnterobacteriaceae\u3c/i\u3e

The detergent-resistance properties of 208 independent isolates of the Enterobacteriaceae have been examined. Of these bacterial strains, 200 were able to grow in the presence of ≥5% sodium dodecyl sulfate, including all members of the Klebsielleae tribe. This resistance does not appear to be plasmid encoded. It is proposed that detergent-resistant organisms he termed saponotolerant or saponophilic, by analogy with other microorganisms occupying harsh ecological niches. In contrast to their prevalent resistance to anionic detergents, not one of the 208 strains tested was found to grow in the presence of three different cationic detergents. This sensitivity to cationic detergents may be of significance in combating nosocomial infections. La resistance it I\u27 action de detergents a ete evaluee chez 208 souches non-apparentees d\u27 Enterobacteriaceae. Deux cents de ces souches bacteriennes incluant tous les membres de la tribu des Klebsielleae ont ete capables de pousser en presence d\u27une concentration ≥5% de dodecyl sulfate de sodium. Cette resistance ne semble pas de nature plasmidique. Pour designer ces organismes resistants aux detergents, il est propose d\u27utiliser les termes saponotolerants ou saponophiles par analogie avec d\u27autres microorganismes qui occupent aussi des niches ecologiques hostiles. Contrastant avec la forte prevalence des souches resistantes aux detergents anioniques, on constate qu\u27aucune des 208 souches n\u27etait capable de pousser en presence de trois detergents cationiques differents. Cette sensibilite aux detergents cationiques pourrait s\u27averer interessante it exploiter dans la lutte aux infections nosocomiales

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Prevalence of extreme detergent resistance among the \u3ci\u3eEnterobacteriaceae\u3c/i\u3e

The detergent-resistance properties of 208 independent isolates of the Enterobacteriaceae have been examined. Of these bacterial strains, 200 were able to grow in the presence of ≥5% sodium dodecyl sulfate, including all members of the Klebsielleae tribe. This resistance does not appear to be plasmid encoded. It is proposed that detergent-resistant organisms he termed saponotolerant or saponophilic, by analogy with other microorganisms occupying harsh ecological niches. In contrast to their prevalent resistance to anionic detergents, not one of the 208 strains tested was found to grow in the presence of three different cationic detergents. This sensitivity to cationic detergents may be of significance in combating nosocomial infections. La resistance it I\u27 action de detergents a ete evaluee chez 208 souches non-apparentees d\u27 Enterobacteriaceae. Deux cents de ces souches bacteriennes incluant tous les membres de la tribu des Klebsielleae ont ete capables de pousser en presence d\u27une concentration ≥5% de dodecyl sulfate de sodium. Cette resistance ne semble pas de nature plasmidique. Pour designer ces organismes resistants aux detergents, il est propose d\u27utiliser les termes saponotolerants ou saponophiles par analogie avec d\u27autres microorganismes qui occupent aussi des niches ecologiques hostiles. Contrastant avec la forte prevalence des souches resistantes aux detergents anioniques, on constate qu\u27aucune des 208 souches n\u27etait capable de pousser en presence de trois detergents cationiques differents. Cette sensibilite aux detergents cationiques pourrait s\u27averer interessante it exploiter dans la lutte aux infections nosocomiales

Flower-Visiting Insect Assemblages on Fall-Blooming Native California Sage Scrub Shrubs

Pollinator studies in the endangered California sage scrub ecosystem have focused on spring insect assemblages, when most plant species bloom. Consequently, the insect assemblages using common fall-blooming sage scrub shrubs Lepidospartum squamatum, Ericameria pinifolia, and Baccharis pilularis remain undescribed. Our study aimed to: (1) document flower-visiting insect assemblages on fall-blooming shrubs, (2) assess the efficacy of three sampling techniques in inventorying insect assemblages, and (3) explore, using DNA metabarcoding, which plants are utilized and the extent to which surrounding suburban habitats’ plants are also used. While elevated sampling is required to inventory flower-visiting insects, we describe a diverse assemblage consisting of 123 species. Insect assemblages differed between L. squamatum and B. pilularis, as well as, E. pinifolia and B. pilularis, but not between L. squamatum and E. pinifolia. Direct sampling approaches (netting and photo documentation) collected 115 species not collected by passive malaise traps, highlighting that active observations are required to describe flower-visiting insect assemblages. Sequencing the ITS2 region of pollen from abundant visitors revealed that a majority of pollen is from the sage scrub ecosystem, highlighting its value. Our results indicate that the presence of fall-blooming shrubs may be critical for maintaining diverse sage scrub insect and pollinator assemblages