Translation of mRNA injected into Xenopus oocytes is specifically inhibited by antisense RNA.

The bacteriophage SP6 promoter and RNA polymerase were used to synthesize sense and antisense RNAs coding for the enzymes thymidine kinase (TK) and chloramphenicol acetyl transferase (CAT). Injection of antisense CAT RNA into frog oocytes inhibited expression of sense CAT mRNA. Similarly, antisense TK RNA inhibited expression of sense TK mRNA. Antisense RNAs were stable in oocytes and had no detectable effect on either the expression of endogenous proteins or on the expression of nonhomologous RNA transcripts. CAT activity expressed from a plasmid transcribed in the oocyte nucleus was also inhibited by antisense RNA injected into the oocyte cytoplasm. The data suggest that antisense RNA will be useful in identifying the function of specific mRNA sequences during early development of the frog

Primitive erythropoiesis in early chick embryogenesis. II. Correlation between hemoglobin synthesis and the mitotic history.

Primitive erythroblasts in the circulating blood of the chick embryo continue to divide while synthesizing hemoglobin (Hb). Hb measurements on successive generations of erythroblasts show that there is a progressive increase in the Hb content of both interphase and metaphase cells. Furthermore, for any given embryo the Hb content of metaphase cells is always significantly greater than that of interphase cells. The distribution of Hb values for metaphase cells suggests that there are six Hb classes corresponding to the number of cell cycles in the proliferative phase. The location of erythroblasts in the cell cycle was determined by combining Feulgen cytophotometry with thymidine radioautography on the same cells. Measurements of the Hb content for erythroblasts in different compartments of the cell cycle (G1, S, G2, and M) show a progressive increase through the cycle. Thus, the amount of Hb per cell is a function of the number of cell divisions since the initiation of Hb synthesis and, to a lesser degree, the stage of the cell cycle. Earlier generations of erythroblasts synthesize Hb at a faster rate than the terminal generation. Several models have been proposed to explain these findings

Pinpointing the Position of the Post-AGB Star at the Core of RAFGL 2688 using Polarimetric Imaging with NICMOS

We have used infrared polarimetric imaging with NICMOS to determine precisely the position of the star that illuminates (and presumably generated) the bipolar, pre-planetary reflection nebula RAFGL 2688 (the Egg Nebula). The polarimetric data pinpoint the illuminating star, which is not detected directly at wavelengths less than or equal to 2 microns, at a position well within the dark lane that bisects the nebula, 0.55" (about 550 AU) southwest of the infrared peak which was previously detected at the southern tip of the northern polar lobe. The inferred position of the central star corresponds to the geometric center of the tips of the four principle lobes of near-infrared H2 emission; identifying the central star at this position also reveals the strong point symmetric structure of the nebula, as seen both in the intensity and polarization structure of the polar lobes. The polarimetric and imaging data indicate that the infrared peak directly detected in the NICMOS images is a self-luminous source and, therefore, is most likely a distant binary companion to the illuminating star. Although present theory predicts that bipolar structure in pre-planetary and planetary nebulae is a consequence of binary star evolution, the separation between the components of the RAFGL 2688 binary system, as deduced from these observations, is much too large for the presence of the infrared companion to have influenced the structure of the RAFGL 2688 nebula.Comment: 15 pages, 6 figures, to appear in The Astrophysical Journa