Seasonal trends in response to inoculation of coast live oak with Phytophthora ramorum

We developed a branch cutting inoculation method to provide a controlled system for studying variation in response to inoculation of coast live oak (Quercus agrifolia) with Phytophthora ramorum. This method has advantages over inoculations of trees in the field, in containing the inoculum and in allowing high levels of replication and the possibility of time series of responses. We previously reported significant tree-to-tree variation, with little population variation in lesion size using this method (Dodd and others 2005). Here we report on a time series in which branch cuttings were collected from the same trees at eight dates through a full year cycle. Branch cuttings were sampled from 33 trees from two sites at China Camp in Marin County, California, including 18 trees from one site that had suffered heavy mortality from this disease (Miwok Meadows) and 15 trees from a second site that has had little infection (Chicken Coop Island)

Yield loss of barley and wheat varieties to Fusarium Crown Rot

Aim To evaluate the relative yield loss (tolerance) of commonly grown and newly released wheat and barley varieties to Fusarium crown rot

Temperature and inoculation method influence disease phenotypes and mortality of Eucalyptus marginata clonal lines inoculated with Phytophthora cinnamomi

Survival of 1-year-old plants of three clonal lines of Eucalyptus marginata (jarrah), two ranked as resistant (RR1 and RR2) and one as susceptible (SS1) to Phytophthora cinnamomi, was assessed after pathogen inoculation with either mycelial mats underbark or zoospores on the stem. Plants were grown at 15, 20, 25 and 30°C. Method of inoculation did not produce comparable mortalities of the clonal lines, particularly at 25 and 30°C. At these temperatures, all three clonal lines had 100% mortality when inoculated underbark, but when inoculated with zoospores, RR1 had 60% survival and lines SS1 and RR2 had 100% mortality. Generally, the level of resistance of all clonal lines declined with increasing temperature. RR2 had consistently higher mortality than SS1, and is therefore not considered resistant. Lesion development was also measured in detached stems of RR1 and a susceptible clonal line (SS2) each inoculated underbark with four different P. cinnamomi isolates. Stems were assessed for lesion development at 20, 25 and 30°C for 4 days. For all four isolates, detached stems of RR1 generally had smaller lesions than those of SS2, particularly at 30°C. The increase in lesion length with increasing temperature was greatest for SS2. Detached stems may have potential in screening for jarrah resistant toP. cinnamomi and allow identification of susceptible clonal lines at 30°C

Cold plasma inactivation of naturally occurring fungi, artificially inoculated Fusarium graminearium and associated mycotoxins in wheat grain

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Cold plasma vs fungicide: exploring options to manage Fusarium head blight and mycotoxin accumulation in wheat grain in the field

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Choosing the best yielding wheat and barley variety under high crown rot

This paper reports on a three year series (2014-2016) of Western region based trials to provide WA grain growers experimental field evidence of the effect of crown rot on barley and wheat variety yields in local environmental conditions

UAV mapping of rhizoctonia bare patch for targeted treatment

Rhizoctonia patches can be easily identified on both normal (RGB) and NDVI images captured by UAV. Mapping shows the pattern of patch distribution across a paddock, highlighting areas of crop with high and low levels of rhizoctonia patches

Crop sequences in Western Australia: what are they and are they sustainable? Findings of a four-year survey

A survey was conducted of commercial broadacre paddocks in the south-west cropping zone of Western Australia from 2010 to 2013. In total, 687 paddock years of data were sampled from 184 paddocks. The land use of each paddock was recorded together with measurements of weed density, the incidence of soilborne pathogen DNA, and soil inorganic nitrogen (nitrate and ammonium). The dynamics of these biophysical variables were related to the crop and pasture sequences employed. Wheat was the most frequent land use (60% of paddock years), followed by canola and pasture (12% each), and lupins and barley (6% each). Four crop species, wheat, canola, barley and lupins, accounted for 84% of land use. By region, wheat, canola, barley and lupin accounted for 90% of land use in the Northern Agricultural Region (NAR), 83% in the Central Agricultural Region (CAR) and 78% in the Southern Agricultural Region (SAR). Conversely, pasture usage in the SAR was 21%, compared with 12% in the CAR and 7% in the NAR. Over the surveyed paddocks, weed density, soilborne pathogens and soil N were maintained at levels suitable for wheat production. The inclusion of land uses other than wheat at the frequency reported maintained the condition of these biophysical variables