We developed a branch cutting inoculation method to provide a controlled system for studying variation in response to inoculation of coast live oak (Quercus agrifolia) with Phytophthora ramorum. This method has advantages over inoculations of trees in the field, in containing the inoculum and in allowing high levels of replication and the possibility of time series of responses. We previously reported significant tree-to-tree variation, with little population variation in lesion size using this method (Dodd and others 2005). Here we report on a time series in which branch cuttings were collected from the same trees at eight dates through a full year cycle. Branch cuttings were sampled from 33 trees from two sites at China Camp in Marin County, California, including 18 trees from one site that had suffered heavy mortality from this disease (Miwok Meadows) and 15 trees from a second site that has had little infection (Chicken Coop Island)
