[Fotografie della Somalia: scuola coranica]

Fotografie appartenenti alla collezione privata di Maurizio Gigli._-_Sawirradii Maurizio Gigli._-_picture belonging to Maurizio Gigli private collection

Quantitative Analyses of the Prognostic Significance of the Hypoxia Response Signature

<div><p>Response signature was analyzed in the 295 breast cancer samples in the NKI study</p> <p>(A) Scatter plots showing the relationship between the value of the average expression level of the genes in the hypoxia signature and that of genes in the wound response signature [<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030047#pmed-0030047-b022" target="_blank">22</a>] or 70-gene signature [<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030047#pmed-0030047-b048" target="_blank">48</a>]. Each point in the scatter plots represents a single one of the 295 tumors analyzed in the NKI dataset. The overall correlation between each pair of expression signatures across this set of 295 samples is indicated in each panel.</p> <p>(B) With the threshold value of the hypoxia response signature for classification of patients into high and low hypoxia response groups set at zero, the Kaplan-Meier curve shows significant differences in survival and time to recurrence between the samples in the high and low hypoxia response groups of these breast cancer samples.</p></div

The Analysis of Hypoxia Response in Human Cancers

<p>The expression values of genes in the “epithelial cell hypoxia signature” were extracted from an expression study of 41 renal tumors (A) [<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030047#pmed-0030047-b028" target="_blank">28</a>]. Genes and samples are organized by hierarchical clustering. The tumors are segregated into two groups defined by high (blue) or low (red) hypoxia response. The histopathological features of renal tumors within the high and low hypoxia response groups are shown. Expression patterns of available “epithelial hypoxia response” genes in a group of breast cancer samples from Norway and Stanford (B) [<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030047#pmed-0030047-b029" target="_blank">29</a>] and NKI (C) [<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030047#pmed-0030047-b047" target="_blank">47</a>] as well as ovarian cancer samples (D) are shown. The tumors are separated into two groups based on their hypoxia responses, high or low. Kaplan-Meier curves for the clinical outcomes of indicated tumors exhibiting high and low hypoxia responses are shown in (B), (C), and (D). In the Kaplan-Meier curve diagrams, high hypoxia response is indicated by blue, low hypoxia response is indicated by red, and censored patients are indicated with “x” marks. The correlation between the average hypoxia response and HIF-1α RNA levels in 70 ovarian cancer samples is shown in (E).</p

Elevated HIF-1α in RPTECs and Its Role in RPTEC Hypoxia Response

<div><p>(A and B) Hierarchical clustering of a total of 3,630 arrays elements that display a greater than 3-fold change in mRNA expression when placed under hypoxic/anoxic environments in more than four different samples among the 56 samples (A). All samples of the same cell lineages segregate themselves into distinct branches (EC, red; HMEC, pink; RPTEC#1 and #2, orange; SM, blue). The names of some representative genes (mitochondrial genes, <i>PHD3, CD31,</i> and genes encoding keratins) are shown to the right, and two elements representing HIF-1α are expanded in (B).</p> <p>(C) The relative expression level of HIF-1α assessed with real-time PCR is shown for different cell types under ambient O₂ concentration.</p> <p>(D) The levels of HIF-1α protein measured by Western blots of different cells under either ambient air (N) or 2% O₂ (H) at the indicated times (6 or 12 h).</p> <p>(E) The level of HIF-1α in RPTECs assessed with real-time PCR after the transfection of either control d-siRNAs (transfection fluid alone [Cont] or Dicer-generated siRNAs generated from GL3 or RL3) or d-siRNAs generated from different regions of HIF-1α mRNAs (RNAi-1, 2, and 3).</p> <p>(F) The comparison of hypoxia responses of RPTECs after treatment with either three control or three HIF-1α RNAi transfections. A cluster of genes sensitive to HIF-1α RNAi treatments is marked by a vertical green bar and is expanded with the names of selected genes shown. All the genes with names shown are induced only in RPTECs during hypoxia (<a href="http://www.plosmedicine.org/article/info:doi/10.1371/journal.pmed.0030047#pmed-0030047-g002" target="_blank">Figure 2</a>B). (Complete data can be found at: <a href="http://microarray-pubs.stanford.edu/hypoxia" target="_blank">http://microarray-pubs.stanford.edu/hypoxia</a>)</p></div

Overview of the Genomic Responses to Hypoxia

<div><p>(A–C) Hierarchical clustering of a total of 4,333 elements that display a greater than 3-fold change in mRNA expression in more than four different samples when exposed to hypoxia (A). Data from individual elements or genes are represented as single rows, and different time points in the time courses (triangles) are shown as columns. Red and green denote expression levels of samples cultured under hypoxia (2% O₂) or anoxia (0% O₂) greater or lower, respectively, than baseline values of samples cultured under ambient air (∼21% O₂). The intensity of the color reflects the magnitude of the change from baseline. The color of the triangles represents the time course of the different cell types (red, ECs; blue, SMCs; pink, HMECs; orange, RPTECs). The vertical red bar marks a cluster of genes induced in all cells, termed the “common hypoxia genes” (B); the vertical brown bar marks a cluster of genes induced in all epithelial cells, termed the “epithelial hypoxia genes”; and the vertical green bar marks a cluster of genes repressed in all cells, termed the “commonly repressed hypoxia genes” (C). The gene clusters representing “common hypoxia genes” (B) and the “commonly repressed hypoxia genes” (C) are expanded to show the names of representative genes on the right side.</p> <p>(D) Average folds of gene induction (y-axis) in the common hypoxia genes cluster from each indicated cell type at different time points (x-axis) are calculated and shown. (Complete data can be found at: <a href="http://microarray-pubs.stanford.edu/hypoxia" target="_blank">http://microarray-pubs.stanford.edu/hypoxia</a>)</p></div