Glycosylation of rat liver nucleoproteins involved in the regulation of haptoglobin gene expression is changed during acute phase response

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The effect of chronic food restriction on liver acute phase protein response in female and male Wistar rats

The acute inflammatory response of the liver associated with chronic food restriction was examined in adult female and male Wistar rats. The changes in the levels of serum markers of liver injury, AST and ALT and the appearance of a serum marker of inflammation, the acute phase protein (APP) haptoglobin (Hp) were assessed following turpentine treatment of well-nourished (WN) controls and undernourished (UN) rats. Undernutrition was induced by food restriction during a six week period by offering chow equivalent to 50% of the normal food intake. In the female rats undernutrition significantly potentiated liver injury and increased their sensitivity to the toxic effects of turpentine, which was opposite to the results obtained for males. Differences in the basal levels of AST, ALT and Hp between females and males imply that the effects of chronic food restriction on protein synthesis in the liver are gender related.Ispitivan je akutni inflamatorni odgovor jetre odraslih ženki i mužjaka Wistar pacova u uslovima hroničnog izgladnjivanja. Praćene su promene koncentracije AST-a i ALT-a kao serumskih markera povrede jetre, i prisustvo akutno faznog proteina haptoglobina-serumskog markera inflamacije, nakon tretiranja normalno hranjenih (WN) i neuhranjenih pacova (UN) terpentinom. Neuhranjenost je bila indukovana restrikcijom hrane u periodu od šest nedelja količinom koja je za 50% bila manja od one koja se normalno uzima. Kod ženki pacova neuhranjenost je značajno potencirala inflamaciju jetre i povećava senzitivnost na toksične efekte terpentina što je suprotno od onog dobijenog za mužjake. Razlike u bazalnim nivoima AST-a, ALT-a i haptoglobina između ženki i mužjaka ukazuju da su efekti hroničnog izgladnjivanja na sistem za proteinsku sintezu u jetri specifični za pol.nul

Distinct effects of virgin coconut oil supplementation on the glucose and lipid homeostasis in non-diabetic and alloxan-induced diabetic rats

Non-diabetic and alloxan-induced diabetic rats were fed with standard laboratory food enriched with 20% virgincoconut oil for 16 weeks. In non-diabetic animals coconut oil improved insulin sensitivity and ability to controlglycaemia and decreased the serum triglycerides for almost 50% in comparison with controls. Supplementationwith coconut oil caused liver steatosis in both non-diabetic and diabetic animals. However, the severity ofsteatosis was lower in diabetic animals compared to non-diabetic animals. Coconut oil had no effects on hearthistology, ascending and abdominal aorta wall thickening and atherosclerotic plaques development neither innon-diabetic nor in diabetic animals. While alloxan treatment caused Type I diabetes in rats, supplementationwith coconut oil in combination with the alloxan unexpectedly resulted in Type II diabetes. The development ofsevere insulin resistance and deterioration in serum lipid profile implied that the use of coconut oil is contra-indicated in diabetic condition

The effect of nutritional status on the magnitude of acute phase protein response in female rats

In response to inflammation, infection and tissue injury, the sequence of events known as acute-phase (AP) response is induced and a number of plasma proteins, called acute-phase proteins (APPs) are synthesized in the liver as a part of the host defense mechanism. Any condition which impairs acute phase protein response (APPR) and hence organism's ability to restore homeostasis may limit its survival in various stress circumstances. Malnutrition is well-known causes of several metabolic, immune and neuroendocrine dysfunctions and this study was aimed at investigating its effect on the magnitude of normal APPR and in relation to turpentine injury. Malnutrition in female Wistar rats was induced by food restriction during six weeks period until they riched and kept body weight to 50% of that of ad libitum fed mates. Results of rocket immunoelectrophoresis revealed that malnutrition alone or in conjunction with turpentine was capable of elevating an expression of one of APPs, haptoglobin (Hp). Based on the studies of liver-specific regulatory proteins that bind to hormone regulatory elements of Hp gene and thus determine its expression we found that malnutrition probably modulated Hp response by changing the expression of active members of C/EBP, STAT and NF-kB transcription factor families.U odgovoru na inflamaciju, infekciju i povredu tkiva, kao sastavni deo odbrambenih mehanizama u organizmu se pokreće sled događaja poznat kao akutno-fazni odgovor (AFO) u okviru koga se u jetri sintetiše grupa proteina plazme označena kao akutno fazni proteini (AFP). Svako stanje koje narušava akutno fazni proteinski odgovor organizma (AFPO), a time i njegovu sposobnost da ponovo uspostavi homeostazu može da deluje ograničavajuće na njegovo preživljavanje u uslovima različitih vrsta stresa. Izgladnjivanje je dobro poznat uzrok nekoliko metaboličkih, imunih i neuroendokrinih poremećaja te je ovaj rad imao za cilj da ispita uticaj neuhranjenosti na nivo normalnog AFPO-a i onog izazvanog akutnom povredom. Ženke pacova soja Wistar su izgladnjivane tokom perioda od šest nedelja sve do dostizanja telesne mase koja predstavlja 50% telesne mase ženki pacova koje su se u istom periodu hranile normalno. Rezultati "roket" imunoelektroforeze su pokazali da je izgladnjivanje, samo ili u sprezi sa terpentinskom povredom dovelo do uvecanja ekspresije jednog od AFP-a, haptoglobina (Hp). Izučavanjem za jetru specifičnih regulatornih proteina koji se vezuju za hormon regulatorne elemente gena Hp i time određuju nivo njegove ekspresije zaključili smo da izgladnjivanje najverovatnije moduliše odgovor Hp-a tako što menja ekspresiju aktivnih članova C/EBP, STAT and NF-kB familija transkripcionih faktora.nul

The effect of nutritional status on the magnitude of acute phase protein response in female rats

In response to inflammation, infection and tissue injury, the sequence of events known as acute-phase (AP) response is induced and a number of plasma proteins, called acute-phase proteins (APPs) are synthesized in the liver as a part of the host defense mechanism. Any condition which impairs acute phase protein response (APPR) and hence organism's ability to restore homeostasis may limit its survival in various stress circumstances. Malnutrition is well-known causes of several metabolic, immune and neuroendocrine dysfunctions and this study was aimed at investigating its effect on the magnitude of normal APPR and in relation to turpentine injury. Malnutrition in female Wistar rats was induced by food restriction during six weeks period until they riched and kept body weight to 50% of that of ad libitum fed mates. Results of rocket immunoelectrophoresis revealed that malnutrition alone or in conjunction with turpentine was capable of elevating an expression of one of APPs, haptoglobin (Hp). Based on the studies of liver-specific regulatory proteins that bind to hormone regulatory elements of Hp gene and thus determine its expression we found that malnutrition probably modulated Hp response by changing the expression of active members of C/EBP, STAT and NF-kB transcription factor families.U odgovoru na inflamaciju, infekciju i povredu tkiva, kao sastavni deo odbrambenih mehanizama u organizmu se pokreće sled događaja poznat kao akutno-fazni odgovor (AFO) u okviru koga se u jetri sintetiše grupa proteina plazme označena kao akutno fazni proteini (AFP). Svako stanje koje narušava akutno fazni proteinski odgovor organizma (AFPO), a time i njegovu sposobnost da ponovo uspostavi homeostazu može da deluje ograničavajuće na njegovo preživljavanje u uslovima različitih vrsta stresa. Izgladnjivanje je dobro poznat uzrok nekoliko metaboličkih, imunih i neuroendokrinih poremećaja te je ovaj rad imao za cilj da ispita uticaj neuhranjenosti na nivo normalnog AFPO-a i onog izazvanog akutnom povredom. Ženke pacova soja Wistar su izgladnjivane tokom perioda od šest nedelja sve do dostizanja telesne mase koja predstavlja 50% telesne mase ženki pacova koje su se u istom periodu hranile normalno. Rezultati "roket" imunoelektroforeze su pokazali da je izgladnjivanje, samo ili u sprezi sa terpentinskom povredom dovelo do uvecanja ekspresije jednog od AFP-a, haptoglobina (Hp). Izučavanjem za jetru specifičnih regulatornih proteina koji se vezuju za hormon regulatorne elemente gena Hp i time određuju nivo njegove ekspresije zaključili smo da izgladnjivanje najverovatnije moduliše odgovor Hp-a tako što menja ekspresiju aktivnih članova C/EBP, STAT and NF-kB familija transkripcionih faktora.nul

Induction of acute phase reaction and suppression of the immune system in the paraoxon-intoxicated rats

Widespread use of organophosphates, especially in agriculture, led us to study their toxic effects. Our investigations were focused on paraoxon an oxygenated analog of a phosphothionate pesticide. This points to an analogy between the response to organophosphate intoxication and the acute phase reaction to injury. The capacity of paraoxon to elicit the acute phase response was assessed by studying two major processes characteristic of acute inflammation, the expression of acute phase proteins (APP) and the immunosuppressive activity of serum. After an LDso paraoxon administration to rats, the serum APP levels increased with time reaching a maximal level at the 24 h time point. The several-fold increases of AGP, MG, Hp and TST concentrations in the circulation of intoxicated rats, as well as a significant immunosuppressive activity of examined animal serum, pointed to the role of APP, especially AGP and MG, as immune modulators. These processes are analog to those observed during the acute phase response to injury and aimed at reestablishing homeostasis.Široka upotreba organofosfatnih jedinjenja u industriji, a posebno u poljoprivredi, navela nas je da ispitujemo njihove toksične efekte. Istraživanja su bila fokusirana ka paraoksonu, kiseoničnom analogu fosfotionatnog pesticida, sa ciljem da se utvrdi analogija između odgovora organizma na intoksikaciju paraoksonom i akutno fazne reakcije na povredu. Sposobnost paraoksona da indukuje akutno fazni odgovor analizirana je ispitivanjem dva glavna procesa karakteristična za inflamaciju: ekspresije akutno faznih proteina i imunosupresivne aktivnosti seruma. Nakon ubrizgavanja LDso paraoksona u pacove, nivo akutno faznih proteina u serumu životinja se povećava dostižući maksimalni nivo u 24 satu. Višestruko povećanje koncentracije al- kiselog glikoproteina (AGP), a2-makroglobulina (MG), haptoglobina i tiostatina u cirkulaciji tretiranih pacova kao i značajna imunosupresivna aktivnost seruma tretiranih životinja ukazali su na ulogu ovih proteina, naročito AGP i MG, kao finih modulatora imunološkog odgovora. Ovi procesi su analogni procesima tokom akutno faznog odgovora organizma na povredu i odvijaju se u cilju uspostavljanja fiziološke ravnoteže.nul

Serum Level of HMGB1 Protein and Inflammatory Markers in Patients with Secondary Peritonitis: Time Course and the Association with Clinical Status

Background: Intra-abdominal infection in secondary peritonitis drives as excessive production of inflammatory mediators and the development of systemic inflammatory response syndrome (SIRS) or sepsis. Finding a specific marker to distinguish SIRS from sepsis would be of immense clinical importance for the therapeutic approach. It is assumed that high-mobility group box 1 protein (HMGB1) could be such a marker. In this study, we examined the time course changes in the blood levels of HMGB1, C-reactive protein (CRP), procalcitonin (PCT) and serum amyloid A (SAA) in patients with secondary peritonitis who developed SIRS or sepsis. Methods: In our study, we evaluated 100 patients with diffuse secondary peritonitis who developed SIRS or sepsis (SIRS and SEPSIS group) and 30 patients with inguinal hernia as a control group. Serum levels of HMGB1, CRP, PCT, and SAA were determined on admission in all the patients, and monitored daily in patients with peritonitis until discharge from hospital. Results: Preoperative HMGB1, CRP, PCT and SAA levels were statistically highly significantly increased in patients with peritonitis compared to patients with inguinal hernia, and significantly higher in patients with sepsis compared to those with SIRS. All four inflammatory markers changed significantly during the follow-up. It is interesting that the patterns of change of HMGB1 and SAA over time were distinctive for SIRS and SEPSIS groups. Conclusions: HMGB1 and SAA temporal patterns might be useful in distinguishing sepsis from noninfectious SIRS in secondary peritonitis.Journal of Medical Biochemistry (2017), 36(1): 44-5

The effect of chronic food restriction on liver acute phase protein response in female and male Wistar rats

The acute inflammatory response of the liver associated with chronic food restriction was examined in adult female and male Wistar rats. The changes in the levels of serum markers of liver injury, AST and ALT and the appearance of a serum marker of inflammation, the acute phase protein (APP) haptoglobin (Hp) were assessed following turpentine treatment of well-nourished (WN) controls and undernourished (UN) rats. Undernutrition was induced by food restriction during a six week period by offering chow equivalent to 50% of the normal food intake. In the female rats undernutrition significantly potentiated liver injury and increased their sensitivity to the toxic effects of turpentine, which was opposite to the results obtained for males. Differences in the basal levels of AST, ALT and Hp between females and males imply that the effects of chronic food restriction on protein synthesis in the liver are gender related.Ispitivan je akutni inflamatorni odgovor jetre odraslih ženki i mužjaka Wistar pacova u uslovima hroničnog izgladnjivanja. Praćene su promene koncentracije AST-a i ALT-a kao serumskih markera povrede jetre, i prisustvo akutno faznog proteina haptoglobina-serumskog markera inflamacije, nakon tretiranja normalno hranjenih (WN) i neuhranjenih pacova (UN) terpentinom. Neuhranjenost je bila indukovana restrikcijom hrane u periodu od šest nedelja količinom koja je za 50% bila manja od one koja se normalno uzima. Kod ženki pacova neuhranjenost je značajno potencirala inflamaciju jetre i povećava senzitivnost na toksične efekte terpentina što je suprotno od onog dobijenog za mužjake. Razlike u bazalnim nivoima AST-a, ALT-a i haptoglobina između ženki i mužjaka ukazuju da su efekti hroničnog izgladnjivanja na sistem za proteinsku sintezu u jetri specifični za pol.nul

Effets motivationnels et sommatifs de l'évaluation par réussite-échec à paliers

Résumé à la fin des ptiesÉgalement disponible en version papierTitre de l'écran-titre (visionné le 3 mars. 2010)Comprend des bibliogr

Altered cytokine expression in Helicobacter pylori infected patients with bleeding duodenal ulcer

Objective Peptic ulcer disease is a condition in which an important role has infection with H. pylori. The most common complication of peptic ulcer is bleeding. The presence of H. pylori triggers local and systemic cytokine signaling which may affect processes such as healing, gastric or duodenal rupture, and carcinogenesis. In this study, we examined the concentrations of IL-1β, IL-6, IL-10, TNF, TGF-β and IL-17A in serum by enzyme immunoassay and their mRNA expressions in periulcer biopsies obtained from patients with bleeding peptic ulcer by means of real-time-PCR. Results We have shown that pro-inflammatory IL-6 and TNF concentrations in serum were significantly higher in patients who were infected with H. pylori, while the concentrations of TGF-β and IL-17A were significantly lower compared to non-infected subjects. IL-17A expression in periulcer mucosa was significantly higher in patients who were infected with H. pylori, while the expression of other cytokines, there was no significant difference compared to non-infected controls. Considering higher serum concentrations in non-infected subjects and higher IL-17A expression in mucosal tissue of infected patients, our data support the studies that found IL-17A has protective role in eradication of H. pylori infection in infected patients