A Method to Measure Saw-Chain Lubrication

A saw-bar with a saw-chain is a common tool in both mechanized and motor-manual harvesting operations. The friction between the saw-bar and the saw-chain must be reduced by lubrication. A precise oil flow control can reduce the amount of oil needed. Traditionally, mineral oils have been used, but the use of biodegradable vegetable-based oils has increased. The goal of this study was to evaluate the lubrication characteristics at different oil flows of two vegetable-based and one mineral-based saw-chain oil. The study was done on an experimental rig with a saw-chain speed of 23 m/s and with pressure between the saw-chain and a rotating rubber roller. The temperature of the saw-bar was used as an indicator of the lubrication efficiency. The saw-chain tension was constant and independent of temperature. In general, increased oil flow resulted in a lower temperature. For rapeseed oil and pine oils, the results were consistently significant between oil flows of 2 and 6 ml/min. At an oil flow of 2 ml/min and 5 minute test time, pine oil resulted in the highest temperature (121°C, standard deviation [SD] 6.4) and at 6 ml/min the lowest temperature (99°C, SD 1.1) compared with the other oils. No difference in temperature was found between mineral oil and rapeseed oils at oil flows of 2 or 6 ml/min. An oil flow of 2 ml/min was found to be enough to prevent high temperatures for all oil types. The study method, with an adjustable experimental rig as the tool, was found to be suitable for studies on lubrication of the saw-bar and saw-chain

Nanoparticles that communicate in vivo to amplify tumour targeting

Author Manuscript: 2012 May 29Nanomedicines have enormous potential to improve the precision of cancer therapy, yet our ability to efficiently home these materials to regions of disease in vivo remains very limited. Inspired by the ability of communication to improve targeting in biological systems, such as inflammatory-cell recruitment to sites of disease, we construct systems where synthetic biological and nanotechnological components communicate to amplify disease targeting in vivo. These systems are composed of ‘signalling’ modules (nanoparticles or engineered proteins) that target tumours and then locally activate the coagulation cascade to broadcast tumour location to clot-targeted ‘receiving’ nanoparticles in circulation that carry a diagnostic or therapeutic cargo, thereby amplifying their delivery. We show that communicating nanoparticle systems can be composed of multiple types of signalling and receiving modules, can transmit information through multiple molecular pathways in coagulation, can operate autonomously and can target over 40 times higher doses of chemotherapeutics to tumours than non-communicating controls.National Cancer Institute (U.S.) (SBMRI Cancer Center Support Grant 5 P30 CA30199-28)National Cancer Institute (U.S.) (MIT CCNE Grant U54 CA119349)National Cancer Institute (U.S.) (Bioengineering Research Partnership Grant 5-R01-CA124427)National Cancer Institute (U.S.) (UCSD CCNE Grant U54 CA 119335)National Science Foundation (U.S.) (Whitaker Graduate Fellowship

Interactions of extracts from selected chewing stick sources with Aggregatibacter actinomycetemcomitans

BACKGROUND: Aggregatibacter actinomycetemcomitans produces a leukotoxin that activates a pro-inflammatory death of human monocytes/macrophages. A specific clone of this bacterium (JP2) has a 530-base pair deletion in the leukotoxin promoter gene that causes a significantly enhanced expression of leukotoxin. This specific clone of A. actinomycetemcomitans is common in some African populations and has a strong association with periodontal attachment loss in adolescents in these populations. Chewing sticks of plant origin are commonly used as oral hygiene tool in Africa, but their role as a therapeutic agent in periodontal disease is poorly investigated. RESULTS: Ethanol extracts were made from 7 common plants used as chewing sticks in West-Africa. None of the tested extracts inhibited growth of A. actinomycetemcomitans. However, extracts from Psidium guajava (Guava) completely neutralized the cell death and pro-inflammatory response of human leukocytes induced by the leukotoxin. None of the six other tested chewing stick extracts showed this effect. CONCLUSIONS: The discovery that extracts from Guava efficiently neutralizes A. actinomycetemcomitans leukotoxicity might lead to novel therapeutic agents and strategies for prevention and treatment of aggressive forms of periodontitis induced by infections with the highly leukotoxic JP2 clone of this bacterium