Comparative chromatography of chloroplast pigment

Methods for isolation of low concentration pigments of the cocklebur species are described. The methods entail two step chromatography so that the different sorption properties of the various pigments in varying column parameters can be utilized. Columnar and thin layer methods are compared. Many conditions influence separability of the chloroplasts

Evaluation of immunization practices in Naples, Italy.

This paper reports the results of a survey on vaccination coverage among children born in January 1995 and residing at the beginning of the study (March 1998) in the city of Naples, Italy. The percentages vaccinated, at various times from birth, with oral polio vaccine (OPV), have been compared with those found in a similar survey conducted at the end of 1985 regarding the cohort of children born in June 1983. By the fourth month of life 67% of the 1995 cohort were vaccinated with the first doses of OPV, an increase of about 26% on that found in the 1983 cohort. Similar results were found with the second doses. Among the 1995 cohort 49% were vaccinated with the third dose of OPV within the thirteenth month of life; the corresponding value for the 1983 cohort was 33%. Within the twenty-fourth month of life, in the 1995 cohort, 86% completed the primary cycle of vaccination with OPV; the corresponding figure for the 1983 cohort was 65%. At the end of the third year of life 80% of the 1995 cohort received the fourth dose of OPV. A significant association has been found between socioeconomic status and coverage level

Deepfield connect, an innovative decision support system for crops irrigation management under Mediterranean conditions

The irrigation management, in the Mediterranean region, represents an important technique useful to reach sustainable yield and improve the quality of the crop. The use of decision support systems and water saving techniques has gained importance during the last decades mainly in arid and semiarid countries where water is considered a precious resource. DeepField Connect by BOSCH is an innovative tool able to support farmers in irrigation management and consists of three main parts: hardware (sensors, device-to-web-data logger and thermo-hygrometer), algorithm and graphic use interface (app). This system is based on GIS analysis, which represents the most innovative and functional tool for such studies, which provides a mapping of soil hydrological characteristics at the regional level. We used, as a reference, soil data analysis obtained at Regional level from the ACLA II Project. In this way, the system creates an interactive mapping system, matching each point of the Apulian surface, in particular, the texture composition of the soil and the values of the hydrological constants (wilting point, WP and field capacity FC), for irrigation planning. These data are integrated with the recharging point (RP) a value calculated for the main regional irrigated crop which represents the level of soil moisture that, together with FC, represent the range of plant-available water. Besides, this tool provides different irrigation strategies such as deficit irrigation or complete restitution of evapotranspiration losses, according to farmer needs. DeepField Connect by BOSCH transmits the data via the Bosch Cloud to the smartphone. This allows to keep track of fields at any given time and to provide assistance in: when to irrigate and which irrigation volumes to use. This intelligent system can be considered as the application of one of the best practices that the agricultural sector can implement to improve its environmental performance and contribute to sustainable food production

Innate Immune Responses to Bacterial Ligands in the Peripheral Human Lung – Role of Alveolar Epithelial TLR Expression and Signalling

It is widely believed that the alveolar epithelium is unresponsive to LPS, in the absence of serum, due to low expression of TLR4 and CD14. Furthermore, the responsiveness of the epithelium to TLR-2 ligands is also poorly understood. We hypothesised that human alveolar type I (ATI) and type II (ATII) epithelial cells were responsive to TLR2 and TLR4 ligands (MALP-2 and LPS respectively), expressed the necessary TLRs and co-receptors (CD14 and MD2) and released distinct profiles of cytokines via differential activation of MAP kinases. Primary ATII cells and alveolar macrophages and an immortalised ATI cell line (TT1) elicited CD14 and MD2-dependent responses to LPS which did not require the addition of exogenous soluble CD14. TT1 and primary ATII cells expressed CD14 whereas A549 cells did not, as confirmed by flow cytometry. Following LPS and MALP-2 exposure, macrophages and ATII cells released significant amounts of TNFα, IL-8 and MCP-1 whereas TT1 cells only released IL-8 and MCP-1. P38, ERK and JNK were involved in MALP-2 and LPS-induced cytokine release from all three cell types. However, ERK and JNK were significantly more important than p38 in cytokine release from macrophages whereas all three were similarly involved in LPS-induced mediator release from TT1 cells. In ATII cells, JNK was significantly more important than p38 and ERK in LPS-induced MCP-1 release. MALP-2 and LPS exposure stimulated TLR4 protein expression in all three cell types; significantly more so in ATII cells than macrophages and TT1 cells. In conclusion, this is the first study describing the expression of CD14 on, and TLR2 and 4 signalling in, primary human ATII cells and ATI cells; suggesting that differential activation of MAP kinases, cytokine secretion and TLR4 expression by the alveolar epithelium and macrophages is important in orchestrating a co-ordinated response to inhaled pathogens

An electron microscopy study of changes in dense core vesicles of PC12 cells following pulse depolarization

Depolarization of PC12 cells by brief pulses of high K+ or ATP produces electrophysiological responses consistent with the quantal release of ATP. Quantitative electron microscopy was used to validate whether the same protocol changed dense core vesicles containing endogenous ATP. The total vesicle number fell by 54% after high K+ and by 21% after ATP. Perimembrane vesicles were significantly depleted by high K+, yet unchanged by ATP, suggesting differential contribution by vesicle pools to distinct stimuli during the release process. Vesicle changes were consistent with vesicular release mechanisms for the liberation of discrete packets of endogenous ATP. These data thus support the use of clustered PC12 cells as a model to study the process of ATP release. (c) 2005 Lippincott Williams D Wilkins