1,001 research outputs found
OA031-01. HIV-1 infection is characterized by early loss of CD161+ Th17 cells and gradual decline in regulatory T cells
An HIV-1 Nef genotype that diminishes immune control mediated by protective human leucocyte antigen alleles
Objectives: Certain human leucocyte antigen (HLA)-B alleles (protective alleles) associate with durable immune control of HIV-1, but with substantial heterogeneity in the level of control. It remains elusive whether viral factors including Nef-mediated immune evasion function diminish protective allele effect on viral control. /
Design: The naturally occurring non-Ser variant at position 9 of HIV-1 subtype C Nef has recently exhibited an association with enhanced HLA-B downregulation function and decreased susceptibility to recognition by CD8+ T cells. We therefore hypothesized this Nef genotype leads to diminished immune control mediated by protective HLA alleles. /
Methods: Nef sequences were isolated from HIV-1 subtype C-infected patients harboring protective alleles and several Nef functions including downregulation of HLA-A, HLA-B, CD4, and SERINC5 were examined. Association between Nef non-Ser9 and plasma viral load was examined in two independent South African and Botswanan treatment-naïve cohorts. /
Results: Nef clones isolated from protective allele+ individuals encoding Nef non-Ser9 variant exhibited greater ability to downregulate HLA-B when compared with the Ser9 variant, while other Nef functions including HLA-A, CD4, and SERINC5 downregulation activity were unaltered. By analyzing a cohort of South African participants chronically infected with subtype C HIV-1, Nef non-Ser9 associated with higher plasma viral load in patients harboring protective alleles. Corroboratively, the Nef non-Ser9 correlated with higher plasma viral load in an independent cohort in Botswana. /
Conclusion: Taken together, our study identifies the Nef genotype, non-Ser9 that subverts host immune control in HIV-1 subtype C infection
P07-02. Cytotoxic T lymphocyte-mediated immune responses in HIV-1 clade C infected mother-child pairs
P16-07. HLA-B1302 is associated with viral control in clade CRF01_AE HIV-1 infection in Thailand
P09-17. Evolution of HLA-B*5703 HIV-1 escape mutations and their impact on HIV-1 replicative capacity
Non-Immunogenicity of Overlapping Gag Peptides Pulsed on Autologous Cells after Vaccination of HIV Infected Individuals
P07-09. Contemporaneous transmission of genetically distinct HIV variants from a single donor to two recipients
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