Allen, TM

Allgaier, RL

Buranapraditkun, S

Chatkulkawin, P

Goulder, P

Hempel, U

Hildebrand, WH

Leitner, T

Lorenzen, S

Matthews, P

Phillips, LC

Pitakpolrat, P

Ruxrungtham, K

Walker, BD

Retrovirology

English

PubMed

Oxford University Research Archive (ORA)

P16-07. HLA-B1302 is associated with viral control in clade CRF01_AE HIV-1 infection in Thailand

Goulder, Philip

Oxford University Research Archive

U Hempel

S Buranapraditkun

P Chatkulkawin

P Pitakpolrat

LC Phillips

RL Allgaier

S Lorenzen

WH Hildebrand

T Leitner

P Matthews

P Goulder

BD Walker

K Ruxrungtham

TM Allen

Springer - Publisher Connector

Crossref

BioMed Central
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(page number not for citation purposes)
Retrovirology
Open AccessPoster presentation
P16-07. HLA-B1302 is associated with viral control in clade 
CRF01_AE HIV-1 infection in Thailand
U Hempel*1, S Buranapraditkun2, P Chatkulkawin2, P Pitakpolrat2, 
LC Phillips1, RL Allgaier1, S Lorenzen2, WH Hildebrand3, T Leitner4, 
P Matthews5, P Goulder5, BD Walker1, K Ruxrungtham2 and TM Allen1
Address: 1Ragon Institute of MGH, MIT and Harvard, Boston, MA, USA, 2Vaccine and Cellular Immunology Laboratory, Chulalongkorn University, 
Bangkok, Thailand, 3Dept. of Microbiology and Immunology, University of Oklahoma, Oklahoma City, OK, USA, 4Theoretical Biology and 
Biophysics, Los Alamos Laboratory, Los Alamos, NM, USA and 5University of Oxford, Oxford, UK
* Corresponding author    
Background
Little is known about the role of different HLA class I alle-
les in the control of HIV-1 CRF01_AE infections, which
dominate in South and Southeast Asia, or the CD8+ T-cell
responses that might be mediating this effect.
Methods
We studied a cohort of 250 chronically HIV-1 infected,
HAART naïve individuals in Bangkok, Thailand. Viral load
and CD4 data were collected for all individuals as well as
4-digit HLA typing and viral sequences. A comprehensive
screen of CD8+ T-cell responses was conducted using an
IFN-gamma ELISpot assay and overlapping clade AE-spe-
cific peptides (OLP) spanning the entire HIV-1 proteome.
Results
Of HLA alleles previously associated with control, namely
B5701, B27, B51 and B1302, only HLA-B1302 was associ-
ated with significantly lower viral loads (n = 14, p = 0.029,
q = 0.115) and with HIV-1 'controllers' (VL<2000/ml; p =
0.016). In examining which CD8 responses might be
mediating this protective effect, we found that the major-
ity of frequently targeted OLPs in HLA-B1302+ subjects
were located in Gag and Pol (7 of top 9 responses; avg.
753 spot forming cells (SFC/106 PBMC). In addition, we
found frequent (avg. 58% recognition) and strong (avg.
872 SFC) responses against 3 of 6 previously described
clade C HLA-B1302 epitopes in Gag and Pol (Gag-VV9,
Pol-GI9, and Gag-RI9), versus infrequent (avg. 12% recog-
nition) and weak (avg. 316 SFC) responses to the other
described epitopes in Pol, Nef and Gag (Pol-RI10, Nef-
GI11, Gag-GI11). In analyzing for CTL escape mutations
in the 3 targeted epitopes or OLPs in Gag, we found no
evidence of viral escape.
Conclusion
HLA-B1302 is correlated with virus control in CRF01_AE
infection and appears to predominantly target highly con-
served regions of Gag and Pol. These data extend the
observation that maintenance of CD8+ T-cell responses
against highly conserved regions of HIV may also be criti-
cal to long-term control of CRF01_AE.
from AIDS Vaccine 2009
Paris, France. 19–22 October 2009
Published: 22 October 2009
Retrovirology 2009, 6(Suppl 3):P236 doi:10.1186/1742-4690-6-S3-P236
<supplement> <title> <p>AIDS Vaccine 2009</p> </title> <editor>Anna Laura Ross</editor> <note>Meeting abstracts – A single PDF containing all abstracts in this Supplement is available <a href="http://www.biomedcentral.com/content/files/pdf/1742-4690-6-S3-full.pdf">here</a>.</note> <url>http://www.biomedcentral.com/content/pdf/1471-2105-10-S12-info.pdf</url> </supplement>
This abstract is available from: http://www.retrovirology.com/content/6/S3/P236
© 2009 Hempel et al; licensee BioMed Central Ltd. 


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P16-07. HLA-B1302 is associated with viral control in clade CRF01_AE HIV-1 infection in Thailand

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