18 research outputs found
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Molecular Cloning of a Bovine Immunoglobulin Lambda Chain cDNA
A cDNA library of the bovine mammary gland constructed in pBR322 was screened by mRNA hybrid-selected translation and by differential hybridization. Several immunoglobulin (Ig) Ξ» light-chain clones were identified and sequenced. Nucleotide sequence comparison of bovine and human Ig Ξ» chains showed a high degree of homology for constant regions and for J regions. The amino acid (aa) sequence encoded by the constant region of the bovine Ig Ξ» chain cDNA contains 107 aa with differences at 24 aa positions from the human Ig A chain. Three complementarity-determining regions (CDR1,2,3) characteristic of the variable region of bovine Ig Ξ» chain cDNA can be distinguished. The bovine and human sequences display good homology in the framework region 3 (FR3) but only patches of homology throughout the FR2 region. The 5β² end of the bovine Ig Ξ» chain cDNA fragment of clone 1-14E contains five stop codons: two in CDR1, one in FR1 and two in the hydrophobic prepeptide region. These data suggest that the IgΞ» mRNA of clone 1-14E is transcribed from the VΞ» pseudogene
Exploiting Domain Knowledge in Making Delegation Decisions
@inproceedings{conf/admi/EmeleNSP11, added-at = {2011-12-19T00:00:00.000+0100}, author = {Emele, Chukwuemeka David and Norman, Timothy J. and Sensoy, Murat and Parsons, Simon}, biburl = {http://www.bibsonomy.org/bibtex/20a08b683088443f1fd36d6ef28bf6615/dblp}, booktitle = {ADMI}, crossref = {conf/admi/2011}, editor = {Cao, Longbing and Bazzan, Ana L. C. and Symeonidis, Andreas L. and Gorodetsky, Vladimir and Weiss, Gerhard and Yu, Philip S.}, ee = {http://dx.doi.org/10.1007/978-3-642-27609-5_9}, interhash = {1d7e7f8554e8bdb3d43c32e02aeabcec}, intrahash = {0a08b683088443f1fd36d6ef28bf6615}, isbn = {978-3-642-27608-8}, keywords = {dblp}, pages = {117-131}, publisher = {Springer}, series = {Lecture Notes in Computer Science}, timestamp = {2011-12-19T00:00:00.000+0100}, title = {Exploiting Domain Knowledge in Making Delegation Decisions.}, url = {http://dblp.uni-trier.de/db/conf/admi/admi2011.html#EmeleNSP11}, volume = 7103, year = 2011 }Postprin
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Identification of Bacterial Clones Encoding Bovine Caseins by Direct Immunological Screening of the cDNA Library
A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were detected by reaction with antiserum to caseins, followed by ^125I-labelled Staphylococcus aureus protein A and autoradiography. Six clones were found positive among 5400 of the cDNA library: 3-A1, 3-B2, 3-B5, 3-H7, 2-A5 and 2-C9. The molecular weights of chimeric pre-Ξ²-lactamase: casein proteins synthesized in
Escherichia coli were estimated by immunoblotting. Colony hybridization and nucleotide sequence analysis showed that clone 3-B5 contained a cDNA fragment of bovine Ο-casein, clone 3-H7 contained a cDNA fragment of Ξ²-casein, while clones 2-A5 and 2-C9 carried cDNA fragments of Ξ±_si-casein
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Identification of Bacterial Clones Encoding Bovine Caseins by Direct Immunological Screening of the cDNA Library
A sensitive immunoassay was used to identify recombinant plasmids carrying cDNA fragments of bovine caseins in the cDNA library from bovine mammary gland mRNA. Colonies grown on nitrocellulose filters were lysed in situ and proteins from the lysates were blotted onto CNBr-activated cellulose filter paper. Antigens covalently bound to CNBr-activated paper or bound to nitrocellulose filters were detected by reaction with antiserum to caseins, followed by ^125I-labelled Staphylococcus aureus protein A and autoradiography. Six clones were found positive among 5400 of the cDNA library: 3-A1, 3-B2, 3-B5, 3-H7, 2-A5 and 2-C9. The molecular weights of chimeric pre-Ξ²-lactamase: casein proteins synthesized in
Escherichia coli were estimated by immunoblotting. Colony hybridization and nucleotide sequence analysis showed that clone 3-B5 contained a cDNA fragment of bovine Ο-casein, clone 3-H7 contained a cDNA fragment of Ξ²-casein, while clones 2-A5 and 2-C9 carried cDNA fragments of Ξ±_si-casein
Dual-Resonator Speed Meter for a Free Test Mass
A description and analysis are given of a ``speed meter'' for monitoring a
classical force that acts on a test mass. This speed meter is based on two
microwave resonators (``dual resonators''), one of which couples evanescently
to the position of the test mass. The sloshing of the resulting signal between
the resonators, and a wise choice of where to place the resonators' output
waveguide, produce a signal in the waveguide that (for sufficiently low
frequencies) is proportional to the test-mass velocity (speed) rather than its
position. This permits the speed meter to achieve force-measurement
sensitivities better than the standard quantum limit (SQL), both when operating
in a narrow-band mode and a wide-band mode. A scrutiny of experimental issues
shows that it is feasible, with current technology, to construct a
demonstration speed meter that beats the wide-band SQL by a factor 2. A concept
is sketched for an adaptation of this speed meter to optical frequencies; this
adaptation forms the basis for a possible LIGO-III interferometer that could
beat the gravitational-wave standard quantum limit h_SQL, but perhaps only by a
factor 1/xi = h_SQL/h ~ 3 (constrained by losses in the optics) and at the
price of a very high circulating optical power --- larger by 1/xi^2 than that
required to reach the SQL.Comment: RevTex: 13 pages with 4 embedded figures (two .eps format and two
drawn in TeX); Submitted to Physical Review
MULTI-AGENT MODELING AND SIMULATION OF DISTRIBUTED DENIAL-OF-SERVICE ATTACKS ON COMPUTER NETWORKS
ΠΠ΅ΡΠ΅Ρ
ΠΎΠ΄ ΠΊ ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ Π²ΠΎΠ΅Π½Π½ΠΎ-ΠΌΠΎΡΡΠΊΠΈΡ
ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΉ, Π±Π°Π·ΠΈΡΡΡΡΠΈΡ
ΡΡ Π½Π° ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΠΏΠ°ΡΠ°Π΄ΠΈΠ³ΠΌΠ΅ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΠΎ-ΡΠ΅Π»Π΅ΠΊΠΎΠΌΠΌΡΠ½ΠΈΠΊΠ°ΡΠΈΠΎΠ½Π½ΠΎΠ³ΠΎ ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π°, ΠΈΠΌΠ΅Π΅Ρ Π½Π΅ΡΠΎΠΌΠ½Π΅Π½Π½ΠΎΠ΅ ΠΏΡΠ΅ΠΈΠΌΡΡΠ΅ΡΡΠ²ΠΎ, ΡΠ²ΡΠ·Π°Π½Π½ΠΎΠ΅ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΠΎΠΉ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΠΎΠΉ ΠΎΠ±ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΈ Π΄Π»Ρ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΏΡΠ΅Π²ΠΎΡΡ
ΠΎΠ΄ΡΡΠ²Π° Π½Π°Π΄ ΠΏΡΠΎΡΠΈΠ²Π½ΠΈΠΊΠΎΠΌ. ΠΠ΄Π½Π°ΠΊΠΎ, ΠΏΡΠΎΡΠΈΠ²Π½ΠΈΠΊ Π±ΡΠ΄Π΅Ρ ΠΏΡΡΠ°ΡΡΡΡ Π°ΡΠ°ΠΊΠΎΠ²Π°ΡΡ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΡΠ΅ ΠΈΠ½ΡΡΠ°ΡΡΡΡΠΊΡΡΡΡ, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠ΅ ΡΠΈΠ»Π°ΠΌΠΈ ΡΠ»ΠΎΡΠΎΠ² Π΄Π»Ρ Π²ΡΠΏΠΎΠ»Π½Π΅Π½ΠΈΡ Π²ΠΎΠ΅Π½Π½ΠΎ-ΠΌΠΎΡΡΠΊΠΈΡ
ΠΎΠΏΠ΅ΡΠ°ΡΠΈΠΉ. ΠΠ΄Π½ΠΎ ΠΈΠ· Π΄Π΅ΠΉΡΡΠ²Π΅Π½Π½ΡΡ
ΡΡΠ΅Π΄ΡΡΠ², ΠΏΡΠΈΠ²ΠΎΠ΄ΡΡΠΈΡ
ΠΊ Π½Π°ΡΡΡΠ΅Π½ΠΈΡ ΡΠ°Π±ΠΎΡΡ ΡΡΠΈΡ
ΠΈΠ½ΡΡΠ°ΡΡΡΡΠΊΡΡΡ, βΡΡΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠ΅ ΠΊΠΎΠΌΠΏΡΡΡΠ΅ΡΠ½ΡΠ΅ Π°ΡΠ°ΠΊΠΈ ΡΠΈΠΏΠ° βΠΡΠΊΠ°Π· Π² ΠΎΠ±ΡΠ»ΡΠΆΠΈΠ²Π°Π½ΠΈΠΈ β (DDoS-Π°ΡΠ°ΠΊΠΈ). ΠΡΠ½ΠΎΠ²Π½Π°Ρ ΡΠ΅Π»Ρ ΡΠ°ΠΊΠΈΡ
Π°ΡΠ°ΠΊ ΡΠΎΡΡΠΎΠΈΡ Π² Π½Π°ΡΡΡΠ΅Π½ΠΈΠΈ ΠΈΠ»ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠΈ Π²ΠΎΠ·ΠΌΠΎΠΆΠ½ΠΎΡΡΠ΅ΠΉ Π΄ΠΎΡΡΡΠΏΠ° Π°Π²ΡΠΎΡΠΈΠ·ΠΎΠ²Π°Π½Π½ΡΡ
ΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°ΡΠ΅Π»Π΅ΠΉ ΠΊ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½Π½ΡΠΌ Π²ΡΡΠΈΡΠ»ΠΈΡΠ΅Π»ΡΠ½ΡΠΌ ΡΠ΅ΡΡΡΡΠ°ΠΌ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΈΡ
ΠΊΠΎΠΌΠΏΡΠΎΠΌΠ΅ΡΠ°ΡΠΈΠΈ. ΠΠΎΠ²ΡΡΠ΅Π½ΠΈΠ΅ ΠΆΠΈΠ²ΡΡΠ΅ΡΡΠΈ ΠΈΠ½ΡΠΎΡΠΌΠ°ΡΠΈΠΎΠ½Π½ΡΡ
ΡΠΈΡΡΠ΅ΠΌ ΠΈ ΠΈΠ½ΡΡΠ°ΡΡΡΡΠΊΡΡΡ Π² ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΡΠ΅Π°Π»ΠΈΠ·Π°ΡΠΈΠΈ ΠΏΡΠΎΡΠΈΠ²Π½ΠΈΠΊΠΎΠΌ ΡΠ°ΠΊΠΈΡ
Π°ΡΠ°ΠΊ ΡΡΠ΅Π±ΡΠ΅Ρ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ Π°Π΄Π΅ΠΊΠ²Π°ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅ΠΎΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ°. ΠΠ΄Π½ΠΎΠΉ ΠΈΠ· Π²Π°ΠΆΠ½ΡΡ
ΡΠΎΡΡΠ°Π²Π»ΡΡΡΠΈΡ
ΡΠ°ΠΊΠΎΠ³ΠΎ ΡΡΠ½Π΄Π°ΠΌΠ΅Π½ΡΠ° ΡΠ²Π»ΡΠ΅ΡΡΡ Π½Π°Π»ΠΈΡΠΈΠ΅ ΡΡΠ΅Π΄ΡΡΠ² ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ DDoS-Π°ΡΠ°ΠΊ. Π€ΠΎΡΠΌΠ°Π»ΡΠ½ΡΠΉ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄ ΠΊ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΠ³ΠΎ ΡΠΏΠ΅ΠΊΡΡΠ° Π°ΡΠ°ΠΊ Π΄Π°Π½Π½ΠΎΠ³ΠΎ ΠΊΠ»Π°ΡΡΠ°, ΠΊΠ»ΡΡΠ΅Π²ΡΠΌΠΈ ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΠΌΠΈ ΠΊΠΎΡΠΎΡΠΎΠ³ΠΎ ΡΠ²Π»ΡΠ΅ΡΡΡ ΠΎΠ½ΡΠΎΠ»ΠΎΠ³ΠΈΡ DDoS-Π°ΡΠ°ΠΊ, ΠΌΠ΅Ρ
Π°Π½ΠΈΠ·ΠΌΡ ΠΊΠΎΠΌΠ°Π½Π΄Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΡΡ
Π°Π³Π΅Π½ΡΠΎΠ², ΡΠ΅Π°Π»ΠΈΠ·ΡΡΡΠΈΡ
DDoS-Π°ΡΠ°ΠΊΠΈ, Π° ΡΠ°ΠΊΠΆΠ΅ ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ½ΡΠΉ ΠΈΠ½ΡΡΡΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠΉ Π΄Π»Ρ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠΊΠΈ ΠΌΠ½ΠΎΠ³ΠΎΠ°Π³Π΅Π½ΡΠ½ΡΡ
ΡΠΈΡΡΠ΅ΠΌ MASDK, ΠΈΡΠΏΠΎΠ»ΡΠ·ΡΠ΅ΠΌΡΠΉ Π΄Π»
Agents and Data Mining Interaction8th International Workshop, ADMI 2012, Valencia, Spain, June 4-5, 2012, Revised Selected Papers /
XII, 217 p. 81 illus.online resource
Agents and Data Mining Interaction7th International Workshop on Agents and Data Mining Interation, ADMI 2011, Taipei, Taiwan, May 2-6, 2011, Revised Selected Papers /
XIII, 361 p.online resource