Application of a mobile measuring device for the planar evaluation of the current in-situ stress condition in glass

The load-bearing capacity of glass as a structural material as well as sustainability and resistance of a built-in glass against appearing loads and forces is assuming an ever-greater importance. Next to analytical and numerical calculations of maximum load-bearing capacity and the ultimate limit state, there is no generally accepted standardized non-destructive inspection method available, with which it is possible to estimate the prevalent load situation and predominant stress conditions, particularly in relation to mechanical or adhered connections. Within the research project “BiGla”, a measuring instrument based on photoelasticity was developed, which enables to measure and monitor occurring load states during the installation process, as well as the utilization of glass components subjected to significant load changes during their life cycle. Based on the combined examination of photoelasticity and its synergism with the finite element analysis it becomes possible to transfer qualitative measurement results into quantitative evaluations of predominant stress conditions. Achieved results, gained during experimental investigations under laboratory conditions, as well as during extensive field tests, are presented in this publication

Comparative analysis of fungal protein kinases and associated domains

<p>Abstract</p> <p>Background</p> <p>Protein phosphorylation is responsible for a large portion of the regulatory functions of eukaryotic cells. Although the list of sequenced genomes of filamentous fungi has grown rapidly, the kinomes of recently sequenced species have not yet been studied in detail. The objective of this study is to apply a comparative analysis of the kinase distribution in different fungal phyla, and to explore its relevance to understanding the evolution of fungi and their taxonomic classification. We have analyzed in detail 12 subgroups of kinases and their distribution over 30 species, as well as their potential use as a classifier for members of the fungal kingdom.</p> <p>Results</p> <p>Our findings show that despite the similarity of the kinase distribution in all fungi, their domain distributions and kinome density can potentially be used to classify them and give insight into their evolutionary origin. In general, we found that the overall representation of kinase groups is similar across fungal genomes, the only exception being a large number of tyrosine kinase-like (TKL) kinases predicted in <it>Laccaria bicolor</it>. This unexpected finding underscores the need to continue to sequence fungal genomes, since many species or lineage-specific properties may remain to be discovered. Furthermore, we found that the domain organization significantly varies between the fungal species. Our results suggest that protein kinases and their functional domains strongly reflect fungal taxonomy.</p> <p>Conclusions</p> <p>Comparison of the predicted kinomes of sequenced fungi suggests essential signaling functions common to all species, but also specific adaptations of the signal transduction networks to particular species.</p

Type 2 Diabetes: Hypoinsulinism, Hyperinsulinism, or Both?

The author discusses a new study reporting the birth weight of patients carrying a mutation in either of two closely related genes associated with maturity-onset diabetes of the young, testing the hypothesis that the primary defect caused by these genes results in decreased insulin secretion

Low Speed Automation, a French Initiative

Nowadays, vehicle safety is constantly increasing thanks to the improvement of vehicle passive and active safety. However, on a daily usage of the car, traffic jams remains a problem. With limited space for road infrastructure, automation of the driving task on specific situation seems to be a possible solution. The French project ABV, which stands for low speed automation, tries to demonstrate the feasibility of the concept and to prove the benefits. In this article, we describe the scientific background of the project and expected outputs. Comment: TRA (2012) Document type: Conference objec

Hyperinsulinism Caused by Paternal-Specific Inheritance of a Recessive Mutation in the Sulfonylurea-Receptor Gene

Neonatal hyperinsulinism (HI) is a genetic disorder of pancreatic b-cells characterized by failure to suppress insulin secretion in the presence of hypoglycemia, resulting in brain damage or death if not adequately treated. Germline mutations in four genes have been associated with HI. Some patients have focal regions of b-cell proliferation (focal HI). Seventy HI probands in whom at least one S U R - 1 mutation was identified were studied. Clinical data from patients with two S U R - 1 mutant alleles were compared with those from patients with single paternally inherited mutations. Thirtyseven probands were homozygous or compound heterozygous for S U R - 1 mutations. In 33 probands, only a single mutation was identified, and in 31, the parental origin of the proband could be determined; in 29, the mutation was on the paternal allele (P \u3c 0.0002). For three of these, pancreatic tissue was available and showed focal b-cell hyperplasia. DNA extracted from the focal lesion and adjacent normal pancreas revealed loss of the maternal chromosome 11p15, resulting in reduction to homozygosity for the S U R - 1 mutation within the focal lesion only. Using the Tdt-mediated dUTP nick end labeling (TUNEL) reaction, apoptotic b-cells were identified exclusively within the focal region. At diagnosis, disease severity was similar in patients with paternally inherited mutations and those with two mutations. For patients who did not undergo surgery, those with only paternal mutations entered clinical remission within 16 ± 6.2 months, compared with 48 ± 23 months for those with two S U R - 1 mutations (P = 0.001). In conclusion, we identified a novel mechanism to explain the pathophysiology of focal HI and provide evidence to suggest that this entity may be self-limiting, since affected b-cells undergo apoptosis

Title

Persistent hypoglycaemia in infancy is most commonly caused by hyperinsulinism. A case is reported of the somatic loss of the maternal 11p in an insulin secreting focal adenoma in association with a germline SUR-1mutation on the paternal allele in a baby boy with hyperinsulinism diagnosed at 49 days old. A reduction to homozygosity of an SUR-1 mutation is proposed as a critical part of the cause of focal hyperinsulinism