Adriana Lecouvreur

De cada obra s'ha digitalitzat un programa sencer. De la resta s'han digitalitzat les parts que són diferents.Director, Gianfranco Masini ; director d'escena, Giuseppe GiulianoEmpresa: Juan A. Pamia

Tectono-sedimentary evolution of the Pliocene to Lower Pleistocene succession of the Apricena-Lesina-Poggio Imperiale quarrying district (western Gargano, southern Italy)

The post-Miocene marine succession of the «Apricena horst» is described with the purpose to verify the chronostratigraphic constraints for the type-locality of the Pirro Nord Faunal Unit. The stratigraphic succession has been subdivided in four units bounded by ubiquitous unconformities with evidence of subaerial exposure. The two basal units (dated late Zanclean to at most early Piacenzian) are formally grouped in the Lago di Varano Fm. that on the whole consists of sediments ranging from lagoonal to circalittoral environments. Within the lowermost unit, a megabreccia is interpreted as the product of a tsunami event. The third unit, Gelasian in age, is informally cited as Calcari a Briozoi Fm. The last unit, the Lower Pleistocene Serracapriola Fm., consists of siliciclastic deltaic sediments and represents the closure of the marine cycle. Conspicuous lateral facies and thickness changes, and the frequency of unconformities are the consequence of an intense synsedimentary tectonic activity developed in the frame of the southern Apulia foredeep closure. In the study area, such activity is documented by two E-W trending normal faults which, during Zanclean and at least the earliest Gelasian, controlled the Pliocene horst-graben system of the Apricena-Poggio Imperiale are

Cannabinoid ReceptorActivation Induces Apoptosis through Tumor Necrosis Factor A Mediated Ceramide De novo Synthesis in Colon Cancer Cells,

PURPOSE: Cannabinoids have been recently proposed as a new family of potential antitumor agents. The present study was undertaken to investigate the expression of the two cannabinoid receptors, CB1 and CB2, in colorectal cancer and to provide new insight into the molecular pathways underlying the apoptotic activity induced by their activation. EXPERIMENTAL DESIGN: Cannabinoid receptor expression was investigated in both human cancer specimens and in the DLD-1 and HT29 colon cancer cell lines. The effects of the CB1 agonist arachinodyl-2'-chloroethylamide and the CB2 agonist N-cyclopentyl-7-methyl-1-(2-morpholin-4-ylethyl)-1,8-naphthyridin-4(1H)-on-3-carboxamide (CB13) on tumor cell apoptosis and ceramide and tumor necrosis factor (TNF)-alpha production were evaluated. The knockdown of TNF-alpha mRNA was obtained with the use of selective small interfering RNA. RESULTS: We show that the CB1 receptor was mainly expressed in human normal colonic epithelium whereas tumor tissue was strongly positive for the CB2 receptor. The activation of the CB1 and, more efficiently, of the CB2 receptors induced apoptosis and increased ceramide levels in the DLD-1 and HT29 cells. Apoptosis was prevented by the pharmacologic inhibition of ceramide de novo synthesis. The CB2 agonist CB13 also reduced the growth of DLD-1 cells in a mouse model of colon cancer. The knockdown of TNF-alpha mRNA abrogated the ceramide increase and, therefore, the apoptotic effect induced by cannabinoid receptor activation. CONCLUSIONS: The present study shows that either CB1 or CB2 receptor activation induces apoptosis through ceramide de novo synthesis in colon cancer cells. Our data unveiled, for the first time, that TNF-alpha acts as a link between cannabinoid receptor activation and ceramide production

sj-docx-1-jtt-10.1177_1357633X231203064 - Supplemental material for iColon, a patient-focused mobile application for perioperative care in colorectal surgery: Results from 444 patients

Supplemental material, sj-docx-1-jtt-10.1177_1357633X231203064 for iColon, a patient-focused mobile application for perioperative care in colorectal surgery: Results from 444 patients by Elisa Bertocchi, Giuliano Barugola, Gaia Masini, Massimo Guerriero, Nicola Menestrina, Irene Gentile, Francesca Meoli, Lorenza Sanfilippo, Mario Lauria, Roberta Freoni and Giacomo Ruffo in Journal of Telemedicine and Telecare</p