HPLC QUANTIFICATION OF LACTOPEROXIDASE IN THERAPEUTIC DAIRY WASTE ENRICHED BY BUBBLESEPARATION

Objective: The objective of this study was to enrich therapeutic proteins and remove pollutants from dairy wastewater for establishing foam fractionation as a lucrative unit operation. Methods: Dairy wastewater collected from dairy industry was processed to fat-free dried protein waste mass diluted to 1-liter feed by distilled water in different concentrations and foam fractionated by sodium dodecyl sulphate (surfactant) to enriched proteins extract (foamate) in a foam fractionator. Foamate were analysed to quantify total proteins and lacto peroxidase respectively. The efficiency was evaluated by varying parameters like pH, initial waste and ionic concentrations, the waste-surfactant mass ratio of feed and flow rate of gas (N2) through feed solution by several experiments. Heat of desorption (λ) and mass transfer coefficient (K) were determined as indicators of adsorptive bubble separation to foam phase governed by Gibb’s equation of adsorption isotherm. Results: The process was optimized at pH 5.5, initial feed concentration 500μg/ml, waste–surfactant mass ratio (1.5:1), gas flow rate (350 ml/min) and ionic concentration 0.1 gram-mole of NaCL per litre of feed with enrichment factor (49.09), percent recovery (98.18%) observed in foamate. One natural preservative specifically lactoperoxidase was quantified by RP-HPLC analysis as 0.49% (w/w) of total proteins at optimal condition. Heat of desorption(λ), mass transfer coefficient(K)were determined 3140cal/mol and 12.68* 10-9 mol/cal/cm2/s respectively at pH 8.5, initial feed concentration 500μg/ml and gas flow rate 350 ml/min. Conclusion: The method may be a useful unit operation for recovery of biomolecules and removal of toxic pollutants from industrial wastewater for coming days

Nature of singularity formed by the gravitational collapse in Husain space-time with electromagnetic field and scalar field

In this work, we have investigated the outcome of gravitational collapse in Husain space-time in the presence of electro-magnetic and a scalar field with potential. In order to study the nature of the singularity, global behavior of radial null geodesics have been taken into account. The nature of singularities formed has been thoroughly studied for all possible variations of the parameters. These choices of parameters has been presented in tabular form in various dimensions. It is seen that irrespective of whatever values of the parameters chosen, the collapse always results in a naked singularity in all dimensions. There is less possibility of formation of a black hole. Hence this work is a significant counterexample of the cosmic censorship hypothesis.Comment: 9 pages, 19 figure

Passive Transdermal Systems Whitepaper Incorporating Current Chemistry, Manufacturing and Controls (CMC) Development Principles

In this whitepaper, the Manufacturing Technical Committee (MTC) of the Product Quality Research Institute has updated the 1997 Transdermal Drug Delivery Systems Scale-Up and Post Approval Change workshop report findings to add important new product development and control principles. Important topics reviewed include ICH harmonization, quality by design, process analytical technologies, product and process validation, improvements to control of critical excipients, and discussion of Food and Drug Administration’s Guidance on Residual Drug in Transdermal and Related Drug Delivery Systems as well as current thinking and trends on in vitro–in vivo correlation considerations for transdermal systems

Gastric adenocarcinoma in a patient re-infected with H. pylori after regression of MALT lymphoma with successful anti-H. pylori therapy and gastric resection: a case report

BACKGROUND: Helicobacter pylori (H. pylori) has been etiologically linked with primary gastric lymphoma (PGL) and gastric carcinoma (GC). There are a few reports of occurrence of both diseases in the same patient with H. pylori infection. CASE PRESENTATION: We report a patient with PGL in whom the tumor regressed after surgical resection combined with eradication of H. pylori infection. However, he developed GC on follow up; this was temporally associated with recrudescence / re-infection of H. pylori. This is perhaps first report of such occurrence. CONCLUSIONS: Possible cause and effect relationship between H. pylori infection and both PGL and GC is discussed. This case also documents a unique problem in management of PGL in tropical countries where re-infection with H. pylori is supposed to be high

An Efficient Algorithm to Compute a Steiner Set and Steiner Tree on Trapezoid Graphs

[[abstract]]This paper presents an ecient algorithm to compute a minimum cardinality Steiner set and Steiner tree on trapezoid graphs. The algo- rithm takes O(m+n p log C) time for a trapezoid graph with n vertices and m edges, where cost of each arc is a non-negative integer number bounded by C

Sun as a cosmic ray TeVatron

Very recently, HAWC observatory discovered the high-energy gamma ray emission from the solar disk during the quiescent stage of the Sun, extending the Fermi-LAT detection of intense, hard emission between 0.1 - 200 GeV to TeV energies. The flux of these observed gamma-rays is significantly higher than that theoretically expected from hadronic interactions of galactic cosmic rays with the solar atmosphere. More importantly, spectral slope of Fermi and HAWC observed gamma ray energy spectra differ significantly from that of galactic cosmic rays casting doubt on the prevailing galactic cosmic ray ancestry model of solar disk gamma rays. In this work, we argue that the quiet Sun can accelerate cosmic rays to TeV energies with an appropriate flux level in the solar chromosphere, as the solar chromosphere in its quiet state probably possesses the required characteristics to accelerate cosmic rays to TeV energies. Consequently, the mystery of the origin of observed gamma rays from the solar disk can be resolved consistently through the hadronic interaction of these cosmic rays with solar matter above the photosphere in a quiet state. The upcoming IceCube-Gen2 detector should be able to validate the proposed model in future through observation of TeV muon neutrino flux from the solar disk. The proposed idea should have major implications on the origin of galactic cosmic rays.Comment: 12 pages, 4 figure

Osteosarcoma of the mandible - second cancer in a case of Hodgkin's lymphoma post-chemotherapy

Osteosarcoma as a second cancer occurring in an adolescent male previously treated with only chemotherapy for Hodgkin\u2032s lymphoma is rare. A 14 year old male presented with gradual swelling of right jaw and numbness of lip and chin for last three months. He was treated with only chemotherapy for Hodgkin\u2032s lymphoma four years back. Incisional biopsy was taken from mandibular swelling, histopathology suggesting osteosarcoma. Right hemimandibulectomy and reconstruction with metallic implant were done along with excision of right parotid lymph node. Chemotherapy was started with cisplatin and doxorubicin regimen. Absence of radiation as a risk factor and very young age of the patient presenting with a second cancer made this case a rarity and hence the case is being reported

Osteosarcoma of the mandible - second cancer in a case of Hodgkin's lymphoma post-chemotherapy

Osteosarcoma as a second cancer occurring in an adolescent male previously treated with only chemotherapy for Hodgkin′s lymphoma is rare. A 14 year old male presented with gradual swelling of right jaw and numbness of lip and chin for last three months. He was treated with only chemotherapy for Hodgkin′s lymphoma four years back. Incisional biopsy was taken from mandibular swelling, histopathology suggesting osteosarcoma. Right hemimandibulectomy and reconstruction with metallic implant were done along with excision of right parotid lymph node. Chemotherapy was started with cisplatin and doxorubicin regimen. Absence of radiation as a risk factor and very young age of the patient presenting with a second cancer made this case a rarity and hence the case is being reported