Additional file 6: Figure S5. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Expression pattern of pWOX2-CENH3-GFP in early megasporogenesis. Representative images of pWOX2-CENH3-GFP fluorescence in early stage megaspores of Arabidopsis thaliana, displaying either the haploid number of five centromeric GFP foci in uninuclear megaspores (A, B and C) or ten GFP signals in binuclear megaspores (D; nuclei are spatially overlapping). Scale bar, 5 μm. (DOC 291 kb

Additional file 3: Table S1. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

CENH3-GFP does not complement cenh3 −/− seed lethality. Genotypic analysis of F2 progeny resulting from several F1 cenh3-1/CENH3 plants that harbor the pWOX2-CENH3-GFP transgene reveals an absence of homozygous cenh3 −/− plants, indicating that cenh3 −/− seed fertility is not restored by the incorporation of the pWOX2 transcribed CENH3-GFP fusion protein. (DOC 25 kb

Additional file 4: Figure S3. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Cenh3-1/CENH3 plants harboring pWOX2-CENH3-GFP show seed abortion. Representative pictures of siliques of Arabidopsis thaliana wild type (A), cenh3-1/CENH3 (B), and cenh3-1/CENH3 plants hemizygous for the pWOX2-CENH3-GFP transgene (C). Heterozygous cenh3-1/CENH3 plants harboring pWOX2-CENH3-GFP show a similar frequency of seed abortion as seen in the control cenh3-1/CENH3 plants, supporting the notion that pWOX2-CENH3-GFP does not complement cenh3 −/− seed abortion. (DOC 425 kb

Additional file 5: Figure S4. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Occasional co-localization of CENH3-GFP foci during microsporogenesis. Representative images of pWOX2-CENH3-GFP fluorescence in uninuclear stage Arabidopsis thaliana microspores. Most microspores clearly exhibit five distinct centromeric GFP foci (A and B), reflecting the haploid chromosome number in diploid Arabidopsis thaliana (2x = 10). However, in some microspores, a lower number of centromeric GFP foci is observed due to the spatial co-localization or Z-axis based projection overlap of two or more GFP foci (C and D; see arrows). Scale bar, 5 μm. (DOC 151 kb

Additional file 7: Figure S6. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

DAPI-stained chromosome spreads of 3x Arabidopsis male meiocytes. DAPI-stained chromosome spreads of somatic (A) and male meiotic (B, C and D) nuclei of triploid Arabidopsis thaliana plants (3x = 15). The somatic nucleus exhibits 15 distinct chromosomes, reflecting the triploid somatic chromosome number. Male meiotic chromosome spreads reveal the formation of five trivalents at diakinesis (B) and metaphase I (C), that inherently lead to an unbalanced segregation of homologous chromosomes at anaphase I (D; 8–7 chromosome segregation). Scale bar, 10 μm. (DOC 206 kb

Additional file 10: Figure S9. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Ploidy distribution of young and mature petals in Arabidopsis thaliana. Histograms representing the DNA ploidy distribution of nuclei isolated from a young, developing petal at flower stage 5–6 (A) and a mature petal at anthesis (B) of a diploid Arabidopsis thaliana plant. (DOC 67 kb

Additional file 8: Figure S7. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Frequency distribution of pWOX2-CENH3-GFP signals in nuclei of late uninuclear and binuclear megaspores isolated from triploid Arabidopsis thaliana plants. (DOC 54 kb

Additional file 11: Table S2. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

Sequences of primers used in this study. (DOC 25 kb