Occasional co-localization of CENH3-GFP foci during microsporogenesis. Representative images of pWOX2-CENH3-GFP fluorescence in uninuclear stage Arabidopsis thaliana microspores. Most microspores clearly exhibit five distinct centromeric GFP foci (A and B), reflecting the haploid chromosome number in diploid Arabidopsis thaliana (2x = 10). However, in some microspores, a lower number of centromeric GFP foci is observed due to the spatial co-localization or Z-axis based projection overlap of two or more GFP foci (C and D; see arrows). Scale bar, 5 μm. (DOC 151 kb
