8 research outputs found
A severe symptom phenotype in pepper cultivars carrying the Tsw resistance gene is caused by a mixed infection between resistance-breaking and non-resistance-breaking isolates of Tomato spotted wilt virus
[EN] Pepper (Capsicum annuum) plants with the
Tsw resistance gene showing unusually severe symptoms
consisting of local lesions, chlorosis, stunting and
systemic necrosis on the apical leaves were found in a
commercial field in north eastern Spain in 2009. The
presence of Tomato spotted wilt virus (TSWV) was
confirmed in all diseased plants. After mechanical inoculation
of Nicotiana glutinosa with infected field samples,
biological clones of the virus were isolated from
individual local lesions. These biological clones produced
two different types of symptoms after inoculation
on Tsw resistant pepper plants: (i) typical symptoms
caused by resistance-breaking (RB) isolates characterized
by chlorosis and stunting, and (ii) severe symptoms
as observed in the field plants. Similar symptoms in
pepper plants carrying the Tsw resistance gene were
reproduced under controlled conditions, after simultaneous
inoculation of RB and non-resistance-breaking
(NRB) isolates. The NRB isolate was detected in a
low proportion in the apical uninoculated leaves, whereas
NRB isolates could not infect resistant pepper when
inoculated alone. Co-infection by NRB and RB isolates
induced disease synergism with systemic necrosis on
the apical leaves. To our knowledge, this is the first case
in which a synergic interaction between isolates of the
same virus has been described, which has the ability to
overcome a natural genetic resistance. This finding
could have epidemiological implications for the management
of TSWVWe thank M. Matas for locating commercial crops of pepper carrying the Tsw gene infected with TSWV and F. Aparicio for his excellent review of the manuscript. This research was supported by grants RTA2008-00010-C03 and RTA2013-00047-C02 from the Instituto Nacional de Investigaciones Agrarias (INIA).Aramburu, J.; Galipienso Torregrosa, L.; Soler Aleixandre, S.; Rubio Miguelez, L.; L贸pez Del Rinc贸n, C. (2015). A severe symptom phenotype in pepper cultivars carrying the Tsw resistance gene is caused by a mixed infection between resistance-breaking and non-resistance-breaking isolates of Tomato spotted wilt virus. Phytoparasitica. 43:597-605. doi:10.1007/s12600-015-0482-1S5976054
A new Capsicum baccatum accession shows tolerance to wild-type and resistance-breaking isolates of Tomato spotted wilt virus
[EN] Tomato spotted wilt virus (TSWV) causes economically important losses in many
crops, worldwide. In pepper (Capsicum annuum), the best method for disease
control has been breeding resistant cultivars by introgression of gene Tsw from
Capsicum chinense. However, this resistance has two drawbacks: (a) it is not
efficient if plants are infected at early growth stages and under prolonged
high temperatures, and (b) it is rapidly overcome by TSWV evolution. In this
work, we selected and evaluated a new accession from Capsicum baccatum,
named PIM26-1, using a novel approach consisting in measuring how three
parameters related to virus infection changed over time, in comparison to
a susceptible pepper variety (Negral) and a resistant (with Tsw) accession
(PI-159236): (a) The level of resistance to virus accumulation was estimated
as an opposite to absolute fitness, W=er
, being r the viral multiplication
rate calculated by quantitative RT-PCR; (b); the level of resistance to virus
infection was estimated as the Kaplan鈥揗eier survival time for no infection
using DAS-ELISA to identify TSWV-infected plants; (c) the level of tolerance
was estimated as the Kaplan鈥揗eier survival time for no appearance of severe
symptoms. Our results showed that the levels of both resistance parameters
against TSWV wild type (WT) and Tsw-resistance breaking (TBR) isolates
were higher in PIM26-1 than in the susceptible pepper variety Negral and
similar to the resistant variety PI-159236 against the TBR isolate. However,
PIM26-1 showed a very high tolerance (none of the plants developed severe
symptoms) to the WT and TBR isolates in contrast to Negral for WT and
TBR or PI-159236 for TBR (most TSWV-inoculated plants developed severe
symptoms). All this indicate that the new accession PIM26-1 is a good candidate
for breeding programmes to avoid damages caused by TSWV TBR isolates
in pepperD.E.D. was the recipient of a fellowship FPU from the Spanish Ministry of Education, Culture and Sports. This work was funded in part by INIA projects RTA2008-00010-C03 and RTA2013-00047-C02. We thank E. Lazaro and Dr. C. Armero (Dept. Statistics and Operational Research, University of Valencia) for helpful suggestions on statistics and Drs. N. Duran and J. Guerri (IVIA) for critical reading of the manuscript.Soler Aleixandre, S.; Debreczeni, DE.; Vidal, E.; Aramburu, J.; L贸pez Del Rinc贸n, C.; Galipienso Torregrosa, L.; Rubio Miguelez, L. (2015). A new Capsicum baccatum accession shows tolerance to wild-type and resistance-breaking isolates of Tomato spotted wilt virus. Annals of Applied Biology. 167:343-353. doi:10.1111/aab.12229S34335316
First report of a natural infection of Eggplant mottled dwarf virus in Podranea ricasoliana showing leaf intervenial yellowing
Alfaro Fern谩ndez, AO.; Font San Ambrosio, MI.; Hern谩ndez Llopis, D.; Galipienso Torregrosa, L.; Rubio, L. (2016). First report of a natural infection of Eggplant mottled dwarf virus in Podranea ricasoliana showing leaf intervenial yellowing. Plant Disease. 100(9):1955-1955. doi:10.1094/PDIS-02-16-0192-PDNS19551955100
Situaci贸n actual de la enfermedad del bronceado despu茅s de 25 a帽os desde su introducci贸n en Espa帽a
[ES] La enfermedad del bronceado del tomate, causada por el Tomato spotted wilt virus (TSWV), fue detectada por primera vez en Espa帽a en el a帽o 1988. A partir de esa fecha, el virus se propag贸 por todo el litoral mediterr谩neo y se convirti贸 en el principal factor limitante para el cultivo de algunas especies hort铆colas. La introducci贸n de resistencias naturales al virus en algunos cultivos comerciales resolvi贸 el problema, pero solo temporalmente ya que en el a帽o 2002 aparecieron variantes virales capaces de superarlas. No obstante, la dispersi贸n de alguna de estas variantes est谩 siendo limitada. La variabilidad gen茅tica del virus, la identificaci贸n de los determinantes gen茅ticos responsables de la rotura de las resistencias, la eficiencia y adaptabilidad de las nuevas variantes virales y las interacciones entre ellas, son aspectos que se discuten en este trabajo, para tratar de explicar la situaci贸n actual de la enfermedad y su posible evoluci贸n en el futuro.Esta investigaci贸n se ha realizado con la aportaci贸n econ贸mica de los proyectos RTA04-004-C2 y RTA2008-00010-C03
del INIA y ACOMP/2011/078 de la Generalitat ValencianaAramburu, J.; Galipienso Torregrosa, L.; L贸pez Del Rinc贸n, C.; Soler Aleixandre, S.; Debreczeni, DE.; Belliure Ferrer, BR. (2013). Situaci贸n actual de la enfermedad del bronceado despu茅s de 25 a帽os desde su introducci贸n en Espa帽a. Phytoma Espa帽a. (251):23-30. http://hdl.handle.net/10251/63983S233025
RNA2鈥恊ncoded VP37 protein of Broad bean wilt virus 1 is a determinant of pathogenicity, host susceptibility, and a suppressor of post鈥恡ranscriptional gene silencing
First report of Tomato leaf curl New Delhi virus infecting cucurbits in the Canary Islands
This work was supported by the Institute Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) grant RTA2017-00061-C03-02 cofunded with FEDER.Espino, A.; Botella, M.; Otazo-Gonz谩lez, H.; Alfaro Fern谩ndez, AO.; Font San Ambrosio, MI.; Galipienso-Torregrosa, L.; Rubio Mig茅lez, L. (2019). First report of Tomato leaf curl New Delhi virus infecting cucurbits in the Canary Islands. Plant Disease. 103(7):1798-1798. https://doi.org/10.1094/PDIS-01-19-0054-PDNS17981798103
Complete sequence of three different biotypes of Tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain
[EN] Tomato spotted wilt virus (TSWV) is worldwide spread and causes production losses in many
important horticultural crops such as tomato and pepper. Breeding resistant cultivars has been the
most successful method for disease control. So far, only two resistance genes have been found to
confer resistance against a wide spectrum of TSWV isolates: Sw-5 in tomato and Tsw in pepper.
However, TSWV resistance-breaking isolates have emerged in different countries after a few
years of implementation of resistant cultivars. In this paper we report the first complete
nucleotide sequence of three TSWV isolates from Spain with different biotypes according to the
ability of overcoming resistance: LL-N.05 (wild type, WT), Pujol1TL3 (Sw-5 resistance
breaking, SBR) and PVR (Tsw resistance-breaking, TBR). The genome of these TSWV isolates
consisted of three genomic segments: L (8913-8914 nt), M (4752-4825 nt) and S (2924-2961 nt).
Variations in nucleotide sequences and genomic lengths among the different virus biotypes are
reported here. Phylogenetic analysis of the five TSWV open reading frames showed evidences of
reassortment between genomic segments of LL-N.05 and Pujol1TL3, which was supported by
analysis with different recombination-detecting algorithms. Genetic distances were uncorrelated
to biotype, host or geographic location.D.E.D. was the recipient of a predoctoral FPU fellowship from the Spanish Ministry of Education, Culture and Sports. This work was funded in part by grants RTA2008-00010-C03 and RTA2013-00047-C02 from Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) and grants ACOMP/2009/103, ACOMP/2010/085 and ACOMP/2011/078 from Generalitat Valenciana. Work in the J.A.D. laboratory was supported by grant BIO2011-26741 from the Spanish Ministerio de Economia y Competitividad. We thank Veronica Aragones (IBMCP, CSIC-UPV) for technical assistance.Debreczeni, DE.; L贸pez Del Rinc贸n, C.; Aramburu, J.; Daros Arnau, JA.; Soler Aleixandre, S.; Galipienso Torregrosa, L.; Falk, BW.... (2015). Complete sequence of three different biotypes of Tomato spotted wilt virus (wild type, tomato Sw-5 resistance-breaking and pepper Tsw resistance-breaking) from Spain. Archives of Virology. 160:2117-2123. https://doi.org/10.1007/s00705-015-2453-8S2117212316
Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan庐MGB probes
A quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) procedure using a general primer set and three TaqMan (R) MGB probes was developed for general and genotype-specific detection and quantitation of the genomic M segment of Tomato spotted wilt virus (TSWV). Standard curves using RNA transcripts homologous to the three probes allowed reproducible quantitative assays with a wide dynamic range (10(3)-10(10) TSWV M segment RNA copies/ng of total RNA) and high sensitivity. This protocol was assayed with a battery of TSWV isolates, covering the range of the present known genetic variation, in single and/or mix infections in three plant hosts, as well as in the thrips vector Frankliniella occidenialis. This quantitative detection assay will be a valuable tool for molecular biology and epidemiology studies, diagnosis and disease control.This work was supported by grants INIA RTA2008-00010-C03 and Generalitat Valenciana ACOMP/2009/103 and ACOMP/2010/085. D.E. Debreczeni is the recipient of a FPU pre-doctoral fellowship from MEC and B. Belliure was supported by a INIA-CCAA contract. We thank I. Ferriol, Dr. S. Ambros and Dr. A. Olmos for technical advice; Dolores Comin for technical assistance; Dr. D. Peters and Dr. J. Contreras for kindly providing the TSWV isolate BR01 and a thrips colony, respectively; and Daniel R. Pearce for English revision.Debreczeni, DE.; Ruiz Ruiz, S.; Aramburu, J.; L贸pez Del Rinc贸n, C.; Belliure Ferrer, BR.; Galipienso Torregrosa, L.; Soler Aleixandre, S.... (2011). Detection, discrimination and absolute quantitation of Tomato spotted wilt virus isolates using real time RT-PCR with TaqMan庐MGB probes. Journal of Virological Methods. 176(1-2):32-37. https://doi.org/10.1016/j.jviromet.2011.05.027S32371761-