Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage

A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3]3+, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]3- as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found, thus confirming a strong effect of MWNT on the enhancement of the electroconductivity of the electrode surface and that of CHIT on the MWNT distribution at the electrode surface. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment. 2008 © The Japan Society for Analytical Chemistry

Impedimetric nanostructured disposable DNA-based biosensors for the detection of deep DNA damage and effect of antioxidants

Novel impedimetric nanostructured disposable DNA-biosensors have been created using a layer of multiwalled carbon nanotubes (MWNT) and double stranded calf thymus or herring sperm DNA deposited on the surface of a screen-printed carbon electrode (SPCE) by layer-by-layer and mixed coverage. The presence of DNA significantly decreases the electroconductivity of the MWNT/SPCE interface and represents a charge barrier for the transport of the [Fe(CN6)]3- redox probe ions. Hence, electrochemical impedimetric procedure performed with DNA/MWNT/SPCE sensor in 0.1 M phosphate buffer solution (PBS) pH 7.0 using 1 mM [Fe(CN6)]3- was developed for the evaluation of deep DNA damage caused by reactive oxygen species formed in situ as well as antioxidative effects of rutin and tea extracts. Good correlation has been found between the charge transfer resistance change obtained as a parameter of the impedimetric equivalent circuit and the voltammetric current response change of the [Fe(CN6)]3- / [Fe(CN6)]4- redox couple measured at the DNA modified and bare SPCEs. © 2008 by ESG

Impedimetric nanostructured disposable DNA-based biosensors for the detection of deep DNA damage and effect of antioxidants

Novel impedimetric nanostructured disposable DNA-biosensors have been created using a layer of multiwalled carbon nanotubes (MWNT) and double stranded calf thymus or herring sperm DNA deposited on the surface of a screen-printed carbon electrode (SPCE) by layer-by-layer and mixed coverage. The presence of DNA significantly decreases the electroconductivity of the MWNT/SPCE interface and represents a charge barrier for the transport of the [Fe(CN6)]3- redox probe ions. Hence, electrochemical impedimetric procedure performed with DNA/MWNT/SPCE sensor in 0.1 M phosphate buffer solution (PBS) pH 7.0 using 1 mM [Fe(CN6)]3- was developed for the evaluation of deep DNA damage caused by reactive oxygen species formed in situ as well as antioxidative effects of rutin and tea extracts. Good correlation has been found between the charge transfer resistance change obtained as a parameter of the impedimetric equivalent circuit and the voltammetric current response change of the [Fe(CN6)]3- / [Fe(CN6)]4- redox couple measured at the DNA modified and bare SPCEs. © 2008 by ESG

Impedimetric nanostructured disposable DNA-based biosensors for the detection of deep DNA damage and effect of antioxidants

Novel impedimetric nanostructured disposable DNA-biosensors have been created using a layer of multiwalled carbon nanotubes (MWNT) and double stranded calf thymus or herring sperm DNA deposited on the surface of a screen-printed carbon electrode (SPCE) by layer-by-layer and mixed coverage. The presence of DNA significantly decreases the electroconductivity of the MWNT/SPCE interface and represents a charge barrier for the transport of the [Fe(CN6)]3- redox probe ions. Hence, electrochemical impedimetric procedure performed with DNA/MWNT/SPCE sensor in 0.1 M phosphate buffer solution (PBS) pH 7.0 using 1 mM [Fe(CN6)]3- was developed for the evaluation of deep DNA damage caused by reactive oxygen species formed in situ as well as antioxidative effects of rutin and tea extracts. Good correlation has been found between the charge transfer resistance change obtained as a parameter of the impedimetric equivalent circuit and the voltammetric current response change of the [Fe(CN6)]3- / [Fe(CN6)]4- redox couple measured at the DNA modified and bare SPCEs. © 2008 by ESG

Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage

A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3]3+, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]3- as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found, thus confirming a strong effect of MWNT on the enhancement of the electroconductivity of the electrode surface and that of CHIT on the MWNT distribution at the electrode surface. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment. 2008 © The Japan Society for Analytical Chemistry

Impedimetric nanostructured disposable DNA-based biosensors for the detection of deep DNA damage and effect of antioxidants

Novel impedimetric nanostructured disposable DNA-biosensors have been created using a layer of multiwalled carbon nanotubes (MWNT) and double stranded calf thymus or herring sperm DNA deposited on the surface of a screen-printed carbon electrode (SPCE) by layer-by-layer and mixed coverage. The presence of DNA significantly decreases the electroconductivity of the MWNT/SPCE interface and represents a charge barrier for the transport of the [Fe(CN6)]3- redox probe ions. Hence, electrochemical impedimetric procedure performed with DNA/MWNT/SPCE sensor in 0.1 M phosphate buffer solution (PBS) pH 7.0 using 1 mM [Fe(CN6)]3- was developed for the evaluation of deep DNA damage caused by reactive oxygen species formed in situ as well as antioxidative effects of rutin and tea extracts. Good correlation has been found between the charge transfer resistance change obtained as a parameter of the impedimetric equivalent circuit and the voltammetric current response change of the [Fe(CN6)]3- / [Fe(CN6)]4- redox couple measured at the DNA modified and bare SPCEs. © 2008 by ESG

Impedimetric nanostructured disposable DNA-based biosensors for the detection of deep DNA damage and effect of antioxidants

Novel impedimetric nanostructured disposable DNA-biosensors have been created using a layer of multiwalled carbon nanotubes (MWNT) and double stranded calf thymus or herring sperm DNA deposited on the surface of a screen-printed carbon electrode (SPCE) by layer-by-layer and mixed coverage. The presence of DNA significantly decreases the electroconductivity of the MWNT/SPCE interface and represents a charge barrier for the transport of the [Fe(CN6)]3- redox probe ions. Hence, electrochemical impedimetric procedure performed with DNA/MWNT/SPCE sensor in 0.1 M phosphate buffer solution (PBS) pH 7.0 using 1 mM [Fe(CN6)]3- was developed for the evaluation of deep DNA damage caused by reactive oxygen species formed in situ as well as antioxidative effects of rutin and tea extracts. Good correlation has been found between the charge transfer resistance change obtained as a parameter of the impedimetric equivalent circuit and the voltammetric current response change of the [Fe(CN6)]3- / [Fe(CN6)]4- redox couple measured at the DNA modified and bare SPCEs. © 2008 by ESG

Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage

A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3]3+, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]3- as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found, thus confirming a strong effect of MWNT on the enhancement of the electroconductivity of the electrode surface and that of CHIT on the MWNT distribution at the electrode surface. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment. 2008 © The Japan Society for Analytical Chemistry

Disposable electrochemical biosensor with multiwalled carbon nanotubes - Chitosan composite layer for the detection of deep DNA damage

A novel electrochemical DNA-based biosensor for the detection of deep DNA damage was designed employing the bionanocomposite layer of multiwalled carbon nanotubes (MWNT) in chitosan (CHIT) deposited on a screen printed carbon electrode (SPCE). The biocomponent represented by double-stranded (ds) herring sperm DNA was immobilized on this composite using layer-by-layer coverage to form a robust film. Individual and complex electrode modifiers are characterized by a differential pulse voltammetry (DPV) with the DNA redox marker [Co(phen)3]3+, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with [Fe(CN)6]3- as a redox probe in a phosphate buffer solution (PBS). A good correlation between the CV and EIS parameters has been found, thus confirming a strong effect of MWNT on the enhancement of the electroconductivity of the electrode surface and that of CHIT on the MWNT distribution at the electrode surface. Differences between the CV and EIS signals of the electrodes without and with DNA are used to detect deep damage to DNA, advantageously using simple working procedures in the same experiment. 2008 © The Japan Society for Analytical Chemistry