Additional file 2: Table S2. of Transcription factors in microalgae: genome-wide prediction and comparative analysis

Sources for the self-build TF database. Table listing sources for the building of the self-build transcription factor database. (XLSX 11 kb

Additional file 1: Table S1. of Transcription factors in microalgae: genome-wide prediction and comparative analysis

Source datasets. Table listing the reference of the genomic data used in this study. (XLSX 11 kb

Relative fatty acid composition of the two new adapted strains in comparison with the initial W2X strain (n = 3; independent culture triplicates) as a % of total fatty acid.

<p>Relative fatty acid composition of the two new adapted strains in comparison with the initial W2X strain (n = 3; independent culture triplicates) as a % of total fatty acid.</p

Temperature conditions applied during the selection experiment.

<p>A cycle was a set of temperature conditions (T_min and T_max) daily applied to culture during selection procedure.</p

Comparison of the total lipids (A) and lipid classes (B) between the adapted Sturb and SFb strains (n = 3; measurement triplicates) and the initial W2X strain (n = 3; independent culture triplicates) after nitrogen starvation.

<p>Comparison of the total lipids (A) and lipid classes (B) between the adapted Sturb and SFb strains (n = 3; measurement triplicates) and the initial W2X strain (n = 3; independent culture triplicates) after nitrogen starvation.</p

Temperature conditions applied during the 3 first cycles of the selection experiment in STurb and SFb.

<p>The same protocol with increasing temperature amplitudes was used for the following cycles (4 to 10).</p

Continuous selection pressure to improve temperature acclimation of <i>Tisochrysis lutea</i> - Fig 3

<p>A) Mean growth rates measured for SFb strain during each selection cycle (black circles), and theoretical growth rate calculated with the calibrated temperature model of the initial strain W2X (triangles) submitted to the same temperature conditions. Growth rate was measured at a minimum of 6 different dates on one culture (SFb, n>6), during the exponential phase to achieve a linear regression with error < 5% and thus an accurate growth rate determination. The vertical dashed lines indicate the amplitude of temperature variations during each cycle, and the horizontal continuous line corresponds to the mean temperature maintained constant at 28°C throughout the selection experiment. B) Time change of the selection coefficient measured in SFb. When measured during consecutive weeks of the same cycle, the selection coefficient increased with time (vertical arrows).</p

Distribution of differential allelic frequency between Tiso-Wt and Tiso-S2M2.

<p>This histogram represents the number of polymorph loci (SNP and indel) following their conservation rate through the selection program. Conservation rate defined as percent represents the differential allelic frequency between Tiso-Wt and Tiso-S2M2.</p

Temperature conditions applied during the 3 first cycles of the selection experiment in STurb and SFb.

<p>The same protocol with increasing temperature amplitudes was used for the following cycles (4 to 10).</p

Comparison between the new STurb and SFb strains and the initial W2X strain.

<p>A) Growth rate. B) Cardinal temperatures and thermal niche. Values are expressed as the mean ± standard deviation determined by a jackknife analysis. Groups marked with the same letter (a, b or c) are not statistically different (1% error, Student test). Different letters indicate groups statistically different.</p