27 research outputs found

    Introduction

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    Additional file 1. R script used for classification with different thresholds of mislabeled observations

    MOESM1 of The use of genetic markers to estimate relationships between dogs in the course of criminal investigations

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    Additional file 1. Average pairwise molecular relatedness. Table of pairwise molecular relatedness between each dog and the dogs in either groups (“Culprits”, “Suspects”), estimated from STR markers

    MOESM1 of “Noisy beets”: impact of phenotyping errors on genomic predictions for binary traits in Beta vulgaris

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    Additional file 1: Figure S1. TPR/TNR variability with all SNP and with subsets of 30 or 50 SNP Distribution of TPR (red) and TNR (blue) in the validation set using KNN and RF with all 175 SNP and with subsets of 50 and 30 SNP. TPR and TNR as a function of mislabeled observations, from a 5-fold cross validation repeated 100 times. Results are presented per method

    Effect of hulled <i>Cannabis sativa</i> L. seeds in a home-made diet for adult dogs

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    Homemade diets integrated with functional ingredients may help to ensure that companion animals have a good life quality given the rise in their average lifespan. This study investigates the effects of a complete and well-balanced homemade diet supplemented with hulled hemp seeds. Twelve adult dogs divided into two groups: CTRL, fed the basal diet and HEMP, fed the diet integrated hulled Cannabis sativa L. seeds (4 g/100g of ration) were enrolled in the trial. The following samples were collected: (a) individual faecal samples to assess the diet digestibility through an indirect method of acid-insoluble ash; b) blood samples to evaluate the oxidative state through an OXI adsorbent test; and c) rectal swabs for intestinal microbiota analysis (alpha- and beta-diversity and taxonomy). The results reveal that with respect to the commercial diet used in this study, our homemade diet increased the total and protein digestibility (total: 53.97 ± 2.54% and 58.20 ± 2.58%; p p p p  The homemade diet increased digestibility and microbiota diversity.Hulled hemp seeds in dog diets increase the body’s defences The homemade diet increased digestibility and microbiota diversity. Hulled hemp seeds in dog diets increase the body’s defences</p

    Additional file 6: of Gut microbiota in experimental murine model of Graves’ orbitopathy established in different environments may modulate clinical presentation of disease

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    Figure S1. NMDS plot based on the weighted Unifrac distances of Center2 immune and control mice including TSHR-immunized mice from Center 1. TSHR-immunized mice from Center 1 were more similar to TSHR-immunized mice from Center 2 (P = 0.2) than to the βgal (P = 0.024) than the untreated (P = 0.04). (PDF 28 kb

    Genome-Wide Association Study for Traits Related to Plant and Grain Morphology, and Root Architecture in Temperate Rice Accessions

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    <div><p>Background</p><p>In this study we carried out a genome-wide association analysis for plant and grain morphology and root architecture in a unique panel of temperate rice accessions adapted to European pedo-climatic conditions. This is the first study to assess the association of selected phenotypic traits to specific genomic regions in the narrow genetic pool of temperate japonica. A set of 391 rice accessions were GBS-genotyped yielding—after data editing—57000 polymorphic and informative SNPS, among which 54% were in genic regions.</p><p>Results</p><p>In total, 42 significant genotype-phenotype associations were detected: 21 for plant morphology traits, 11 for grain quality traits, 10 for root architecture traits. The FDR of detected associations ranged from 3 · 10<sup>−7</sup> to 0.92 (median: 0.25). In most cases, the significant detected associations co-localised with QTLs and candidate genes controlling the phenotypic variation of single or multiple traits. The most significant associations were those for flag leaf width on chromosome 4 (<i>FDR</i> = 3 · 10<sup>−7</sup>) and for plant height on chromosome 6 (<i>FDR</i> = 0.011).</p><p>Conclusions</p><p>We demonstrate the effectiveness and resolution of the developed platform for high-throughput phenotyping, genotyping and GWAS in detecting major QTLs for relevant traits in rice. We identified strong associations that may be used for selection in temperate irrigated rice breeding: e.g. associations for flag leaf width, plant height, root volume and length, grain length, grain width and their ratio. Our findings pave the way to successfully exploit the narrow genetic pool of European temperate rice and to pinpoint the most relevant genetic components contributing to the adaptability and high yield of this germplasm. The generated data could be of direct use in genomic-assisted breeding strategies.</p></div
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