8 research outputs found
Estudio psicométrico piloto de un instrumento para evaluar el funcionamiento familiar: corresponsabilidad y consenso
The goal of this study was the validation of an instrument to assess co-responsibility strategies and family agreement. Considering positive parenting as framework, communication, conflict negotiation and emotions constituted central axes. 220 parents completed an 18 Likert-item instrument. The reliability and validity of the instrument were analyzed: internal consistency, differential items functioning and the analysis of measurement invariance. A 3-factor structure was revealed: beliefs about motherhood and fatherhood, emotional communication and family agreement. Configurational invariance was maintained but not metric and scalar ones. Gender, educational level and labor occupation seemed to determine the parenting competence to be mother and father.El objetivo del estudio es validar un inventario que evalúa las dinámicas de corresponsabilidad y consenso familiar, donde la comunicación, la negociación de conflictos y las emociones son ejes centrales de la parentalidad positiva. 220 progenitores respondieron a un instrumento de 18 ítems. Se analizaron fiabilidad y validez de la prueba: consistencia interna, análisis diferencial de los ítems e invarianza de la medida. Se confirmaron 3 factores: imagen de maternidad/paternidad, comunicación emocional y consenso familiar; se mantuvo la invarianza configuracional pero no la métrica ni escalar. Género, nivel de estudios y ocupación laboral determinarían la competencia parental de ser padre y madre
Estudio psicométrico piloto de un instrumento para evaluar el funcionamiento familiar: corresponsabilidad y consenso
The goal of this study was the validation of an instrument to assess co-responsibility strategies and family agreement. Considering positive parenting as framework, communication, conflict negotiation and emotions constituted central axes. 220 parents completed an 18 Likert-item instrument. The reliability and validity of the instrument were analyzed: internal consistency, differential items functioning and the analysis of measurement invariance. A 3-factor structure was revealed: beliefs about motherhood and fatherhood, emotional communication and family agreement. Configurational invariance was maintained but not metric and scalar ones. Gender, educational level and labor occupation seemed to determine the parenting competence to be mother and father.El objetivo del estudio es validar un inventario que evalúa las dinámicas de corresponsabilidad y consenso familiar, donde la comunicación, la negociación de conflictos y las emociones son ejes centrales de la parentalidad positiva. 220 progenitores respondieron a un instrumento de 18 ítems. Se analizaron fiabilidad y validez de la prueba: consistencia interna, análisis diferencial de los ítems e invarianza de la medida. Se confirmaron 3 factores: imagen de maternidad/paternidad, comunicación emocional y consenso familiar; se mantuvo la invarianza configuracional pero no la métrica ni escalar. Género, nivel de estudios y ocupación laboral determinarían la competencia parental de ser padre y madre
Clinical management of plant food allergy in patients sensitized to lipid transfer proteins is heterogeneous: identifying the gaps
Background and objective: Patients sensitized to lipid transfer protein (LTP)
present a wide clinical variability. The lack of practical diagnostic and therapeutic
guidelines complicate their management. The aim of the study was to describe
the clinical approach of Spanish allergists to this pathology using a survey
designed by PICO method and subsequent Delphi approach validation.
Methods: Designed survey was answered by 224 allergists (75% women; 57.1%
with >20 years of professional experience). Homogeneity regarding clinical
practice on the main points of LTP allergy diagnosis was observed, except for
patients with suspected NSAID hypersensitivity (44.6% frequently include LTP
skin testing). Oral food challenges were not frequently performed (63.6%
occasionally to never), and they were generally (75.5%) used to confirm
tolerance. It was common to recommend fruit skins avoidance (77.2%) and
maintaining consumption of foods to which patients are sensitised but tolerant
(99.1%).
Results: There was heterogeneity on other dietary indications, modifications due
to co-factors, or traces avoidance. Peach sublingual immunotherapy (SLIT) was
considered very/quite effective by 55.9% of allergists. The majority (79.5%)
consider SLIT indicated in <25% of LTP allergic patients, based on severity
(95.2%), frequency of reactions (99.4%), allergy to multiple food families (97.4%),
and the quality of life/nutrition impairment (91.5%). There was different practice
on SLIT prescription based on co-factor involvement.
Conclusion: These data suggest that there is a need to increase evidence to
reduce the clinical practice heterogeneity in the management of LTP allergy
Splicing events in the control of genome integrity: role of SLU7 and truncated SRSF3 proteins
Genome instability is related to disease development and carcinogenesis. DNA lesions are caused
by genotoxic compounds but also by the dysregulation of fundamental processes like transcription,
DNA replication and mitosis. Recent evidence indicates that impaired expression of RNA-binding proteins results in mitotic aberrations and the formation of transcription-associated RNA–DNA hybrids
(R-loops), events strongly associated with DNA injury. We identify the splicing regulator SLU7 as a key
mediator of genome stability. SLU7 knockdown results in R-loops formation, DNA damage, cell-cycle
arrest and severe mitotic derangements with loss of
sister chromatid cohesion (SCC). We define a molecular pathway through which SLU7 keeps in check the
generation of truncated forms of the splicing factor
SRSF3 (SRp20) (SRSF3-TR). Behaving as dominant
negative, or by gain-of-function, SRSF3-TR impair
the correct splicing and expression of the splicing
regulator SRSF1 (ASF/SF2) and the crucial SCC protein sororin. This unique function of SLU7 was found
in cancer cells of different tissue origin and also in
the normal mouse liver, demonstrating a conserved
and fundamental role of SLU7 in the preservation
of genome integrity. Therefore, the dowregulation of
SLU7 and the alterations of this pathway that we observe in the cirrhotic liver could be involved in the
process of hepatocarcinogenesis
Splicing events in the control of genome integrity: role of SLU7 and truncated SRSF3 proteins
Genome instability is related to disease development and carcinogenesis. DNA lesions are caused
by genotoxic compounds but also by the dysregulation of fundamental processes like transcription,
DNA replication and mitosis. Recent evidence indicates that impaired expression of RNA-binding proteins results in mitotic aberrations and the formation of transcription-associated RNA–DNA hybrids
(R-loops), events strongly associated with DNA injury. We identify the splicing regulator SLU7 as a key
mediator of genome stability. SLU7 knockdown results in R-loops formation, DNA damage, cell-cycle
arrest and severe mitotic derangements with loss of
sister chromatid cohesion (SCC). We define a molecular pathway through which SLU7 keeps in check the
generation of truncated forms of the splicing factor
SRSF3 (SRp20) (SRSF3-TR). Behaving as dominant
negative, or by gain-of-function, SRSF3-TR impair
the correct splicing and expression of the splicing
regulator SRSF1 (ASF/SF2) and the crucial SCC protein sororin. This unique function of SLU7 was found
in cancer cells of different tissue origin and also in
the normal mouse liver, demonstrating a conserved
and fundamental role of SLU7 in the preservation
of genome integrity. Therefore, the dowregulation of
SLU7 and the alterations of this pathway that we observe in the cirrhotic liver could be involved in the
process of hepatocarcinogenesis
Splicing events in the control of genome integrity: role of SLU7 and truncated SRSF3 proteins
Genome instability is related to disease development and carcinogenesis. DNA lesions are caused
by genotoxic compounds but also by the dysregulation of fundamental processes like transcription,
DNA replication and mitosis. Recent evidence indicates that impaired expression of RNA-binding proteins results in mitotic aberrations and the formation of transcription-associated RNA–DNA hybrids
(R-loops), events strongly associated with DNA injury. We identify the splicing regulator SLU7 as a key
mediator of genome stability. SLU7 knockdown results in R-loops formation, DNA damage, cell-cycle
arrest and severe mitotic derangements with loss of
sister chromatid cohesion (SCC). We define a molecular pathway through which SLU7 keeps in check the
generation of truncated forms of the splicing factor
SRSF3 (SRp20) (SRSF3-TR). Behaving as dominant
negative, or by gain-of-function, SRSF3-TR impair
the correct splicing and expression of the splicing
regulator SRSF1 (ASF/SF2) and the crucial SCC protein sororin. This unique function of SLU7 was found
in cancer cells of different tissue origin and also in
the normal mouse liver, demonstrating a conserved
and fundamental role of SLU7 in the preservation
of genome integrity. Therefore, the dowregulation of
SLU7 and the alterations of this pathway that we observe in the cirrhotic liver could be involved in the
process of hepatocarcinogenesis
Prediction of long-term outcomes of HIV-infected patients developing non-AIDS events using a multistate approach
Outcomes of people living with HIV (PLWH) developing non-AIDS events (NAEs) remain poorly defined. We aimed to classify NAEs according to severity, and to describe clinical outcomes and prognostic factors after NAE occurrence using data from CoRIS, a large Spanish HIV cohort from 2004 to 2013. Prospective multicenter cohort study. Using a multistate approach we estimated 3 transition probabilities: from alive and NAE-free to alive and NAE-experienced ("NAE development"); from alive and NAE-experienced to death ("Death after NAE"); and from alive and NAE-free to death ("Death without NAE"). We analyzed the effect of different covariates, including demographic, immunologic and virologic data, on death or NAE development, based on estimates of hazard ratios (HR). We focused on the transition "Death after NAE". 8,789 PLWH were followed-up until death, cohort censoring or loss to follow-up. 792 first incident NAEs occurred in 9.01% PLWH (incidence rate 28.76; 95% confidence interval [CI], 26.80-30.84, per 1000 patient-years). 112 (14.14%) NAE-experienced PLWH and 240 (2.73%) NAE-free PLWH died. Adjusted HR for the transition "Death after NAE" was 12.1 (95%CI, 4.90-29.89). There was a graded increase in the adjusted HRs for mortality according to NAE severity category: HR (95%CI), 4.02 (2.45-6.57) for intermediate-severity; and 9.85 (5.45-17.81) for serious NAEs compared to low-severity NAEs. Male sex (HR 2.04; 95% CI, 1.11-3.84), ag
Effectiveness of the combination elvitegravir/cobicistat/tenofovir/emtricitabine (EVG/COB/TFV/FTC) plus darunavir among treatment-experienced patients in clinical practice : A multicentre cohort study
Background: The aim of this study was to investigate the effectiveness and tolerability of the combination elvitegravir/cobicistat/tenofovir/emtricitabine plus darunavir (EVG/COB/TFV/FTC + DRV) in treatment-experienced patients from the cohort of the Spanish HIV/AIDS Research Network (CoRIS). Methods: Treatment-experienced patients starting treatment with EVG/COB/TFV/FTC + DRV during the years 2014-2018 and with more than 24 weeks of follow-up were included. TFV could be administered either as tenofovir disoproxil fumarate or tenofovir alafenamide. We evaluated virological response, defined as viral load (VL) < 50 copies/ml and < 200 copies/ml at 24 and 48 weeks after starting this regimen, stratified by baseline VL (< 50 or ≥ 50 copies/ml at the start of the regimen). Results: We included 39 patients (12.8% women). At baseline, 10 (25.6%) patients had VL < 50 copies/ml and 29 (74.4%) had ≥ 50 copies/ml. Among patients with baseline VL < 50 copies/ml, 85.7% and 80.0% had VL < 50 copies/ml at 24 and 48 weeks, respectively, and 100% had VL < 200 copies/ml at 24 and 48 weeks. Among patients with baseline VL ≥ 50 copies/ml, 42.3% and 40.9% had VL < 50 copies/ml and 69.2% and 68.2% had VL < 200 copies/ml at 24 and 48 weeks. During the first 48 weeks, no patients changed their treatment due to toxicity, and 4 patients (all with baseline VL ≥ 50 copies/ml) changed due to virological failure. Conclusions: EVG/COB/TFV/FTC + DRV was well tolerated and effective in treatment-experienced patients with undetectable viral load as a simplification strategy, allowing once-daily, two-pill regimen with three antiretroviral drug classes. Effectiveness was low in patients with detectable viral loads