Molecular Technique as A Vigorous Instrument for Identification and Classification of Dickeya on The Ornamental Plants

Information on the genetic diversity of pathogenic bacteria causing plant disease is rare to find. This study aims to identify and classify disease-causing pathogens in several ornamental plants molecularly. Sixteen bacterial strains were isolated from carnation, chrysanthemum, and kalanchoe. All strains were grouped in D. dianthicola although the carnation strains and kalanchoe strains gave different results from the identified carnation strains. The differences were found in rep-PCR and 16S rDNA. Band patterns produced by rep-ERIC PCR revealed that the carnation strains, chrysanthemum strains, and kalanchoe strains formed slightly different from the identified carnation strains. Meanwhile, sequence analysis of 16S rDNA revealed that the carnation strains and kalanchoe strains were grouped separately from the identified carnation strains. Even though they were positioned independently from the identified carnation strains and other D. dianthicola strains, but they have closely related each other thus they are identified as a specific group of D. dianthicola. However, based on multilocus sequence analysis (MLSA) of dnaX, recA, gyrB and rpoD, all strains were grouped into D. dianthicola.  Furthermore, the result of pathogenicity test showed that all strains were pathogenic to carnation, potato, and chrysanthemum, but they were not pathogenic to kalanchoe

Study of Hemoglobin Levels on Hemolysis Sample

Hemolysis can significantly affect the reliability of test results and occur in the pre-analytical phase. The aim of this study is to reveals the correlation of hemoglobin levels on hemolysis sample. This experimental study was carried out using samples from thirty students of Medical Laboratory Technology study program of the Nahdlatul Ulama University of Surabaya. Blood samples were treated by hard shaken in 30 times in order to damage the middle part of the blood sample. Data on hemoglobin levels were collected and analyzed using the Pearson Correlation Test before and after treatment with significant value p < 0.05 indicating that there was a significant correlation. Hemoglobin concentrations were strongly positive for the hemolysis of the sample (p = 0.000). The conclusion is that sample hemolysis has a potency to be the confounding factor on the hemoglobin test

Identifikasi Colletotrichum asianum Penyebab Antraknosa Mangga Kultivar Golek di Indramayu

Penyakit antraknosa merupakan salah satu kendala utama dalam budidaya mangga. Penyakit ini sangat merugikan produksi mangga terutama pada saat lepas panen. Gejala antraknosa teramati pada mangga kultivar Golek di gudang simpan petani di Kabupaten Indramayu. Penelitian ini ditujukan untuk mengidentifikasi jenis cendawan fitopatogen penyebab penyakit antraknosa mangga sebagai langkah awal menyusun pengendalian yang efektif. Morfologi cendawan fitopatogen dikarakterisasi secara makroskopis dan mikroskopis. Identifikasi patogen dilakukan dengan teknik molekuler berbasis multilokus dengan penanda wilayah ITS dan gen actin. Cendawan fitopatogen teridentifikasi sebagai Colletotrichum asianum berdasarkan karakterisasi morfologi dan identifikasi molekuler. Anthracnose disease is one of the major obstacles in mango cultivation. This disease is highly detrimental to mango production, especially during the post-harvest period. Symptoms of anthracnose disease were observed on the Golek mango cultivar in the farmer’s warehouse in Indramayu District. The aim of this study was to identify the phytopathogenic fungi that cause mango anthracnose disease as the first step in developing effective control measures. The morphology of the phytopathogenic fungi was characterized both macroscopically and microscopically. Pathogen identification was performed using multilocus molecular techniques with ITS and actin gene region markers. The phytopathogenic fungi were identified as Colletotrichum asianum based on morphological characterization and molecular identification. Koch's postulates of the species were confirmed through a pathogenicity test.Penyakit antraknosa merupakan salah satu kendala utama dalam budidaya mangga. Penyakit ini sangat merugikan produksi mangga terutama pada saat lepas panen. Gejala antraknosa teramati pada mangga kultivar Golek di gudang simpan petani di Kabupaten Indramayu. Penelitian ini ditujukan untuk mengidentifikasi jenis cendawan fitopatogen penyebab penyakit antraknosa mangga sebagai langkah awal menyusun pengendalian yang efektif. Morfologi cendawan fitopatogen dikarakterisasi secara makroskopis dan mikroskopis. Identifikasi patogen dilakukan dengan teknik molekuler berbasis multilokus dengan penanda wilayah ITS dan gen actin. Cendawan fitopatogen teridentifikasi sebagai Colletotrichum asianum berdasarkan karakterisasi morfologi dan identifikasi molekuler. Anthracnose disease is one of the major obstacles in mango cultivation. This disease is highly detrimental to mango production, especially during the post-harvest period. Symptoms of anthracnose disease were observed on the Golek mango cultivar in the farmer’s warehouse in Indramayu District. The aim of this study was to identify the phytopathogenic fungi that cause mango anthracnose disease as the first step in developing effective control measures. The morphology of the phytopathogenic fungi was characterized both macroscopically and microscopically. Pathogen identification was performed using multilocus molecular techniques with ITS and actin gene region markers. The phytopathogenic fungi were identified as Colletotrichum asianum based on morphological characterization and molecular identification. Koch's postulates of the species were confirmed through a pathogenicity test