Alignment file of NPC1 protein sequences_Figure2

Sequences of NPC1 proteins aligned using the MAFFT program. This alignment was used to generate a phylogenetic tree using the phyML 3.0 sofware, the maximum likelihood method and the LG+I+G substitution model (I=0.1 and gamma factor=1.266). Accession numbers: HsNPC1L1, NP_001095118.1; BtNPC1L1, XP_588051; DrNPC1L1, XP_002663230.2; CiNPC1-b, XP_002122922; DmNPC1-a, NP_609357; DmNPC1-b, NP_608417; HsNPC1, AAK25791.1; BtNPC1, AAI51277; DrNPC1, XP_001919958.3; CiNPC1-a, XP_002120129; SpNPC1, XP_780036; AvNPC1, HG670297; NvNPC1, XM_001634881.1; AdNPC1, adi_v1.19271 from ADIG_G-PEP_111201 database; HmNPC1, XM_002164383.1; AqNPC1, XP_003384854.1

PrimersEfficiency

PCR efficiency of primers used in this study (DNA quantification and heat stress experiment

TreeFile_NPC1_Figure2

Tree data generated using the maximum likelihood method and the PhyML3.0 software. Compute these data with the SeaView program to display the NPC1 phylogenetic tree

TreeFile_NPC2_Figure1

Tree data generated using the maximum likelihood method and the PhyML3.0 software. Compute these data with the SeaView program to display the NPC2 phylogenetic tree

qPCR_QuantifZxAv

Symbiodinium quantification by real-time quantitative PCR measurement of the relative nuclear gene copy number (symbiont to host nuclei) during hyperthermal stress

qPCR_HeatStress

Relative gene expression data analysis (raw Ct and normalized expression data) for the heat stress experimen

Normalizationfactor_HeatStress

Normalization factor was calculated based on the expression level of RCC2 and COP-ɣ genes using the GeNorm software, for the heat stress experiment

Alignment file of NPC2 protein sequences_Figure1A

Sequences of NPC2 proteins aligned using the MAFFT program. This alignment was used to construct a phylogenetic tree using the maximum likelihood method (phyML3.0 software) and the WAG+I+G substitution model (I=0.057 and gamma factor=5.831). Accession number of sequences: AvNPC2-d,HG670301; AvNPC2-c, HG670300; NvNPC2-2, XP_001627355; NvNPC2-3, XP_001635502; AdNPC2-2, adi_v1.06781; AdNPC2-3, adi_v1.23434; AdNPC2-4, adi_v1.10342; AvNPC2-b, HG670299; AdNPC2-1, adi_v1.14987 + contig adi_v1.08570; AdNPC2-5, aug_v2a.08569; AdNPC2-6, aug_v2a.08570; AvNPC2-a, HG670298; NvNPC2-1, XP_001622874; HsNPC2, NP_006423.1; DrNPC2-a, NP_001122191.1; DrNPC2-b, NP_775331.1; CiNPC2-1, XP_002127695.1; TaNPC2, XP_002109765.1; HvNPC2-3, XP_002166396.2; Hv NPC2-4, XP_002166374.2; Hv NPC2-2, XP_002164043.1; Hv NPC2-1, XP_002156875.1; AqNPC2, CL5422Contig1 from AQUE_CAP3_100104 assembly from compage