3,559 research outputs found
Macroecological patterns in flower colour are shaped by both biotic and abiotic factors
There is a wealth of research on the way interactions with pollinators shape flower traits. However, we have much more to learn about influences of the abiotic environment on flower colour. We combine quantitative flower colour data for 339 species from a broad spatial range covering tropical, temperate, arid, montane and coastal environments from 9.25ºS to 43.75ºS with 11 environmental variables to test hypotheses about how macroecological patterns in flower colouration relate to biotic and abiotic conditions. Both biotic community and abiotic conditions are important in explaining variation of flower colour traits on a broad scale. The diversity of pollinating insects and the plant community have the highest predictive power for flower colouration, followed by mean annual precipitation and solar radiation. On average, flower colours are more chromatic where there are fewer pollinators, solar radiation is high, precipitation and net primary production are low, and growing seasons are short, providing support for the hypothesis that higher chromatic contrast of flower colours may be related to stressful conditions. To fully understand the ecology and evolution of flower colour, we should incorporate the broad selective context that plants experience into research, rather than focusing primarily on effects of plant–pollinator interactions
A single nucleotide polymorphism in the Bax gene promoter affects transcription and influences retinal ganglion cell death
Pro-apoptotic Bax is essential for RGC (retinal ganglion cell)
death. Gene dosage experiments in mice, yielding a single wild-type
Bax allele, indicated that genetic background was able to
influence the cell death phenotype. DBA/2JBax+/− mice exhibited complete resistance to nerve damage after 2 weeks
(similar to Bax−/− mice), but 129B6Bax+/− mice exhibited significant cell loss (similar to wild-type mice). The
different cell death phenotype was associated with the level of
Bax expression, where 129B6 neurons had twice the level of
endogenous Bax mRNA and protein as DBA/2J neurons. Sequence
analysis of the Bax promoters between these strains revealed a
single nucleotide polymorphism (T129B6 to CDBA/2J) at
position −515. A 1.5- to 2.5-fold increase in transcriptional
activity was observed from the 129B6 promoter in transient transfection assays
in a variety of cell types, including RGC5 cells derived from rat RGCs. Since
this polymorphism occurred in a p53 half-site, we investigated the requirement
of p53 for the differential transcriptional activity. Differential
transcriptional activity from either 129B6 or DBA/2J Bax
promoters were unaffected in p53−/− cells, and
addition of exogenous p53 had no further effect on this difference, thus a role
for p53 was excluded. Competitive electrophoretic mobility-shift assays
identified two DNA–protein complexes that interacted with the
polymorphic region. Those forming Complex 1 bound with higher affinity to the
129B6 polymorphic site, suggesting that these proteins probably comprised a
transcriptional activator complex. These studies implicated quantitative
expression of the Bax gene as playing a possible role in
neuronal susceptibility to damaging stimuli
How large should whales be?
The evolution and distribution of species body sizes for terrestrial mammals
is well-explained by a macroevolutionary tradeoff between short-term selective
advantages and long-term extinction risks from increased species body size,
unfolding above the 2g minimum size induced by thermoregulation in air. Here,
we consider whether this same tradeoff, formalized as a constrained
convection-reaction-diffusion system, can also explain the sizes of fully
aquatic mammals, which have not previously been considered. By replacing the
terrestrial minimum with a pelagic one, at roughly 7000g, the terrestrial
mammal tradeoff model accurately predicts, with no tunable parameters, the
observed body masses of all extant cetacean species, including the 175,000,000g
Blue Whale. This strong agreement between theory and data suggests that a
universal macroevolutionary tradeoff governs body size evolution for all
mammals, regardless of their habitat. The dramatic sizes of cetaceans can thus
be attributed mainly to the increased convective heat loss is water, which
shifts the species size distribution upward and pushes its right tail into
ranges inaccessible to terrestrial mammals. Under this macroevolutionary
tradeoff, the largest expected species occurs where the rate at which
smaller-bodied species move up into large-bodied niches approximately equals
the rate at which extinction removes them.Comment: 7 pages, 3 figures, 2 data table
Human Mas-related G protein-coupled receptors-X1 induce chemokine receptor 2 expression in rat dorsal root ganglia neurons and release of chemokine ligand 2 from the human LAD-2 mast cell line
Primate-specific Mas-related G protein-coupled receptors-X1 (MRGPR-X1) are highly enriched in dorsal root ganglia (DRG) neurons and induce acute pain. Herein, we analyzed effects of MRGPR-X1 on serum response factors (SRF) or nuclear factors of activated T cells (NFAT), which control expression of various markers of chronic pain. Using HEK293, DRG neuron-derived F11 cells and cultured rat DRG neurons recombinantly expressing human MRGPR-X1, we found activation of a SRF reporter gene construct and induction of the early growth response protein-1 via extracellular signal-regulated kinases-1/2 known to play a significant role in the development of inflammatory pain. Furthermore, we observed MRGPR-X1-induced up-regulation of the chemokine receptor 2 (CCR2) via NFAT, which is considered as a key event in the onset of neuropathic pain and, so far, has not yet been described for any endogenous neuropeptide. Up-regulation of CCR2 is often associated with increased release of its endogenous agonist chemokine ligand 2 (CCL2). We also found MRGPR-X1-promoted release of CCL2 in a human connective tissue mast cell line endogenously expressing MRGPR-X1. Thus, we provide first evidence to suggest that MRGPR-X1 induce expression of chronic pain markers in DRG neurons and propose a so far unidentified signaling circuit that enhances chemokine signaling by acting on two distinct yet functionally co-operating cell types. Given the important role of chemokine signaling in pain chronification, we propose that interruption of this signaling circuit might be a promising new strategy to alleviate chemokine-promoted pain
Pulmonary Embolism Incidence and Fatality Trends in Chinese Hospitals from 1997 to 2008: A Multicenter Registration Study
BACKGROUND: There has not been sufficient evidence to support the Asians being less susceptible to pulmonary embolism (PE) than other ethnicities, because the prevalence of PE/deep venous thrombosis (DVT) in different racial and ethnic groups has not been carefully studied until recently except in Caucasians. To test the hypothesis that the Chinese population has a lower risk for PE, this study comprehensively assessed the hospital-based incidence and case fatality rates for PE during the 1997-2008 in China. METHODS: A registration study of patients with suspected PE syndromes admitted to 60 level-3 hospitals involved in the National Cooperative Project for the Prevention and Treatment of Venous Thromboembolism (NCPPT) was conducted from January 1997 to December 2008. The only exclusion criterion was an age of less than 18 years. Helical computed tomography scan, ventilation-perfusion lung scintigraphy or pulmonary angiography was carried out before or after hospitalization. All images were reviewed and evaluated independently by two specialists. RESULTS: A total of 18,206 patients were confirmed with PE from 16,972,182 hospital admissions. The annual incidence was 0.1% (95% CI: 0.1% to 0.2%). The overall incidence of PE in male patients (0.2%, 95% CI: 0.1% to 0.3%) was higher than that in female patients (0.1% and 95% CI: 0.0% to 0.1%). An increasing incidence gradient for PE was noticed from Southern to Northern China. In addition, the case fatality rate was apparently decreasing: 25.1% (95% CI: 16.2% to 36.9%) in 1997 to 8.7% (95% CI: 3.5% to 15.8%) in 2008. CONCLUSIONS: Our findings suggest the relatively stable PE incidence and decreasing fatality trends in Chinese hospitals may be partially attributable to the implementation of the NCCPT and suggest the government should reevaluate the severity of PE so that health resources for the prevention, diagnosis and treatment of PE could be used to their fullest
Seasonal change in the daily timing of behaviour of the common vole, Microtus arvalis
1. Seasonal effects on daily activity patterns in the common vole were established by periodic trapping in the field and continuous year round recording of running wheel and freeding activity in cages exposed to natural meteorological conditions.
2. Trapping revealed decreased nocturnality in winter as compared to summer. This was paralelled by a winter reduction in both nocturnal wheel running and feeding time in cages.
3. Frequent trap checks revealed a 2 h rhythm in daytime catches in winter, not in summer. Cage feeding activity in daytime was always organized in c. 2 h intervals, but day-to-day variations in phase blurred the rhythm in summer in a summation of individual daily records. Thus both seasonal and short-term temporal patterns are consistent between field trappings and cage feeding records.
4. Variables associated with the seasonal change in daily pattern were: reproductive state (sexually active voles more nocturnal), age (juveniles more nocturnal), temperature (cold days: less nocturnal), food (indicated by feeding experiments), habitat structure (more nocturnal in habitat with underground tunnels).
5. Minor discrepancies between field trappings and cage feeding activity can be explained by assuming increased trappability of voles in winter. Cage wheel running is not predictive of field trapping patterns and is thought to reflect behavioral motivations not associated with feeding but with other activities (e.g., exploratory, escape, interactive behaviour) undetected by current methods, including radiotelemetry and passage-counting.
6. Winter decrease in nocturnality appears to involve a reduction in nocturnal non-feeding and feeding behaviour and is interpreted primarily as an adaptation to reduce energy expenditure in adverse but socially stable winter conditions.
Epiparasitic plants specialized on arbuscular mycorrhizal fungi
Over 400 non-photosynthetic species from 10 families of vascular plants obtain their carbon from fungi and are thus defined as myco-heterotrophs. Many of these plants are epiparasitic on green plants from which they obtain carbon by 'cheating' shared mycorrhizal fungi. Epiparasitic plants examined to date depend on ectomycorrhizal fungi for carbon transfer and exhibit exceptional specificity for these fungi, but for most myco-heterotrophs neither the identity of the fungi nor the sources of their carbon are known. Because many myco-heterotrophs grow in forests dominated by plants associated with arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), we proposed that epiparasitism would occur also between plants linked by AMF. On a global scale AMF form the most widespread mycorrhizae, thus the ability of plants to cheat this symbiosis would be highly significant. We analysed mycorrhizae from three populations of Arachnitis uniflora (Corsiaceae, Monocotyledonae), five Voyria species and one Voyriella species (Gentianaceae, Dicotyledonae), and neighbouring green plants. Here we show that non-photosynthetic plants associate with AMF and can display the characteristic specificity of epiparasites. This suggests that AMF mediate significant inter-plant carbon transfer in nature
Warps and waves in the stellar discs of the Auriga cosmological simulations
Recent studies have revealed an oscillating asymmetry in the vertical structure of the Milky Way's disc. Here, we analyse 16 high-resolution, fully cosmological simulations of the evolution of individual MilkyWay-sized galaxies, carried out with the magnetohydrodynamic code AREPO. At redshift zero, about 70 per cent of our galactic discs show strong vertical patterns, with amplitudes that can exceed 2 kpc. Half of these are typical 'integral sign' warps. The rest are oscillations similar to those observed in the Milky Way. Such structures are thus expected to be common. The associated mean vertical motions can be as large as 30 km s-1. Cold disc gas typically follows the vertical patterns seen in the stars. These perturbations have a variety of causes: close encounters with satellites, distant fly-bys of massive objects, accretion of misaligned cold gas from halo infall or from mergers. Tidally induced vertical patterns can be identified in both young and old stellar populations, whereas those originating from cold gas accretion are seen mainly in the younger populations. Galaxies with regular or at most weakly perturbed discs are usually, but not always, free from recent interactions with massive companions, although we have one case where an equilibrium compact disc reforms after a merger
Congenital absence of inferior vena cava and thrombosis: a case report
<p>Abstract</p> <p>Introduction</p> <p>A congenitally absent Inferior Vena Cava (IVC) is a rare anomaly that is recognised to be associated with idiopathic Deep Venous Thrombosis (DVT), particularly in the young. It may not be apparent until later in life. Retrospectively, as discussed in this case, there can be clues indicating the presence of such an anomaly from a young age. However, it is not clear whether early recognition of this condition would affect the prognosis and treatment.</p> <p>Case presentation</p> <p>A 54 year old gentleman was admitted with 3 weeks of abdominal pain and localised swelling over the right flank. Examination revealed palpable 'snake-like' tortuous, tender lumps on the abdominal wall. Past history revealed chronic non-healing venous leg ulcers, and varicose veins necessitating varicose vein ligation at a very young age. The ulcers eventually needed skin grafting.</p> <p>During this, current admission he was investigated and diagnosed with Deep Vein Thrombosis (DVT). CT scan, performed to search for intra-abdominal cancer, revealed absence of the Inferior Vena Cava with extensive thrombosed collaterals of the superficial abdominal and azygous veins and a congenitally atrophic left kidney.</p> <p>Conclusion</p> <p>This is a case of one of the oldest patient described in the literature to be diagnosed with absence of the IVC. It is thought that IVC anomalies are under-diagnosed, and may be commoner than once believed. However there were vital clues in his previous medical history suspicious for an underlying venous anomaly. Idiopathic DVT in a relatively young person with a past history of chronic leg ulceration or varicose veins should be investigated for congenital anomalies of the IVC. This is best achieved by CT scan of the abdomen.</p
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