Investigation of Damper Valve Dynamics Using Parametric Numerical Methods

The objectives of this study are to identify the dynamics of a Tenneco Automotive hydraulic damper valve and to predict valve performance. Accurate simulations of damper valve performance can be used to improve valve designs without the expense of physical testing. The Tenneco damper valve consists of thin shims and a spring preloaded disc that restricts fluid from exiting the main flow orifices. The deflection of the shims and spring are dependent on the flow-rate through the valve. The pressure distribution acting on the deformable valve components is investigated numerically using a dynamic modelling technique. This technique involves sequential geometry and simulation updating, while varying both the geometry and flow-rate. The valve deflection is calculated by post-processing the pressure distribution. Valve performance can be predicted by coupling the valve deflection with CFD pressure results

Componential coding in the condition monitoring of electrical machines Part 2: application to a conventional machine and a novel machine

This paper (Part 2) presents the practical application of componential coding, the principles of which were described in the accompanying Part 1 paper. Four major issues are addressed, including optimization of the neural network, assessment of the anomaly detection results, development of diagnostic approaches (based on the reconstruction error) and also benchmarking of componential coding with other techniques (including waveform measures, Fourier-based signal reconstruction and principal component analysis). This is achieved by applying componential coding to the data monitored from both a conventional induction motor and from a novel transverse flux motor. The results reveal that machine condition monitoring using componential coding is not only capable of detecting and then diagnosing anomalies but it also outperforms other conventional techniques in that it is able to separate very small and localized anomalies

Fatigue analysis of wind turbine gearbox bearings using SCADA data and miner's rule

This paper presents studies of wind turbine load estimation, based upon SCADA data, to derive bearing load histograms for the high speed shaft (HSS) of a typical, three-stage, wind turbine gearbox. Rain-flow counting has been applied to determine the number of loading occurrences at different load values. Damage ratios for bearings have been predicted using this data by the application of Miner's rule. Results show that the bearings have an overload Cycle Ratio of less than 20% above rated and overloads of Load Ratio of 1.0~1.1 incurring significant damage to the bearings

Quantitative Proteomics Identifies the Myb-Binding Protein p160 as a Novel Target of the von Hippel-Lindau Tumor Suppressor

Background: The von Hippel-Lindau (VHL) tumor suppressor gene encodes a component of a ubiquitin ligase complex, which is best understood as a negative regulator of hypoxia inducible factor (HIF). VHL ubiquitinates and degrades the a subunits of HIF, and this is proposed to suppress tumorigenesis and tumor angiogenesis. However, several lines of evidence suggest that there are unidentified substrates or targets for VHL that play important roles in tumor suppression. Methodology/Principal Findings: Employing quantitative proteomics, we developed an approach to systematically identify the substrates of ubiquitin ligases and using this method, we identified the Myb-binding protein p160 as a novel substrate of VHL. Conclusions/Significance: A major barrier to understanding the functions of ubiquitin ligases has been the difficulty in pinpointing their ubiquitination substrates. The quantitative proteomics approach we devised for the identification of VHL substrates will be widely applicable to other ubiquitin ligases

Tandem E2F Binding Sites in the Promoter of the p107 Cell Cycle Regulator Control p107 Expression and Its Cellular Functions

The retinoblastoma tumor suppressor (Rb) is a potent and ubiquitously expressed cell cycle regulator, but patients with a germline Rb mutation develop a very specific tumor spectrum. This surprising observation raises the possibility that mechanisms that compensate for loss of Rb function are present or activated in many cell types. In particular, p107, a protein related to Rb, has been shown to functionally overlap for loss of Rb in several cellular contexts. To investigate the mechanisms underlying this functional redundancy between Rb and p107 in vivo, we used gene targeting in embryonic stem cells to engineer point mutations in two consensus E2F binding sites in the endogenous p107 promoter. Analysis of normal and mutant cells by gene expression and chromatin immunoprecipitation assays showed that members of the Rb and E2F families directly bound these two sites. Furthermore, we found that these two E2F sites controlled both the repression of p107 in quiescent cells and also its activation in cycling cells, as well as in Rb mutant cells. Cell cycle assays further indicated that activation of p107 transcription during S phase through the two E2F binding sites was critical for controlled cell cycle progression, uncovering a specific role for p107 to slow proliferation in mammalian cells. Direct transcriptional repression of p107 by Rb and E2F family members provides a molecular mechanism for a critical negative feedback loop during cell cycle progression and tumorigenesis. These experiments also suggest novel therapeutic strategies to increase the p107 levels in tumor cells

Reliability Analysis for Wind Turbines

Modern wind turbines are complex aerodynamic, mechanical and electrical machines incorporating sophisticated control systems. Wind turbines have been erected in increasing numbers in Europe, the USA and elsewhere. In Europe, Germany and Denmark have played a particularly prominent part in developing the technology, and both countries have installed large numbers of turbines. This article is concerned with understanding the historic reliability of modern wind turbines. The prime objective of the work is to extract information from existing data so that the reliability of large wind turbines can be predicted, particularly when installed offshore in the future. The article uses data collected from the Windstats survey to analyse the reliability of wind turbine components from historic German and Danish data. Windstats data have characteristics common to practical reliability surveys; for example, the number of failures is collected for each interval but the number of turbines varies in each interval. In this article, the authors use reliability analysis methods which are not only applicable to wind turbines but relate to any repairable system. Particular care is taken to compare results from the two populations to consider the validity of the data. The main purpose of the article is to discuss the practical methods of predicting large-wind-turbine reliability using grouped survey data from Windstats and to show how turbine design, turbine configuration, time, weather and possibly maintenance can affect the extracted results

Reliability of wind turbine subassemblies

We have investigated the reliability of more than 6000 modern onshore wind turbines and their subassemblies in Denmark and Germany over 11 years and particularly changes in reliability of generators, gearboxes and converters in a subset of 650 turbines in Schleswig Holstein, Germany. We first start by considering the average failure rate of turbine populations and then the average failure rates of wind turbine subassemblies. This analysis yields some surprising results about which subassemblies are the most unreliable. Then we proceed to consider the failure intensity function variation with time for wind turbines in one of these populations, using the Power Law Process, of three subassemblies; generator, gearbox and converter. This analysis shows that wind turbine gearboxes seem to be achieving reliabilities similar to gearboxes outside the wind industry. However, wind turbine generators and converters are both achieving reliabilities considerably below that of other industries but the reliability of these subassemblies improves with time. The paper also considers different wind turbine concepts. Then we conclude by proposing that offshore wind turbines should be subject to more rigorous reliability improvement measures, such as more thorough subassembly testing, to eliminate early failures. The early focus should be on converters and generators

A B-myb promoter corepressor site facilitates in vivo occupation of the adjacent E2F site by p107-E2F and p130-E2F complexes

International audienceTranscription from the B-myb (MybL2 gene) promoter is strictly cell cycle-regulated by repression mediated through an E2F site during G(0)/early G(1). We report here the characterization of a corepressor site (downstream repression site (DRS)) required for this activity that is closely linked to the E2F site. Systematic mutagenesis of the DRS enabled a consensus to be derived, and it is notable that this sequence is compatible with cell cycle gene homology region sequences associated with cell cycle-dependent elements in the cyclin A, cdc2, and CDC25C promoters. The B-myb promoter is inappropriately active during Go in mouse embryo fibroblasts lacking the p107 and p130 pocket proteins, and we show that the ability of transfected p107 and p130 to re-impose repression on the promoter is dependent on the DRS. In contrast, transfected Rb was unable to repress the B-myb promoter. Consistent with the notion that Rb-E2F complexes are unable to bind the B-myb promoter E2F site in vivo, footprinting showed that this site is unoccupied in cells lacking p107 and p130. Chromatin immunoprecipitation assays showed a requirement for the DRS in recruiting p107 and p130 complexes to the B-myb promoter, indicating that in vivo the DRS governs the occupancy of the adjacent E2F site by transcriptional repressors