2 research outputs found
Core/Shell Nanofibers with Embedded Liposomes as a Drug Delivery System
The broader application of liposomes in regenerative
medicine is
hampered by their short half-life and inefficient retention at the
site of application. These disadvantages could be significantly reduced
by their combination with nanofibers. We produced 2 different nanofiber-liposome
systems in the present study, that is, liposomes blended within nanofibers
and core/shell nanofibers with embedded liposomes. Herein, we demonstrate
that blend electrospinning does not conserve intact liposomes. In
contrast, coaxial electrospinning enables the incorporation of liposomes
into nanofibers. We report polyvinyl alcohol-core/poly-ε-caprolactone-shell
nanofibers with embedded liposomes and show that they preserve the
enzymatic activity of encapsulated horseradish peroxidase. The potential
of this system was also demonstrated by the enhancement of mesenchymal
stem cell proliferation. In conclusion, intact liposomes incorporated
into nanofibers by coaxial electrospinning are very promising as a
drug delivery system
Platelet lysate as a serum replacement for skin cell culture on biomimetic PCL nanofibers
<p>Platelets are a popular source of native growth factors for tissue engineering applications. The aim of the study was to verify the use of platelet lysate as a fetal bovine serum (FBS) replacement for skin cell culture. The cytokine content of the platelet lysate was characterized using the Bio-Plex system. The cells (fibroblasts, melanocytes, and keratinocytes) were cultured on PCL nanofibrous scaffolds to mimic their natural microenvironment. The cytokine content of the platelet lysate was determined, and to the cells, a medium containing platelet lysate or platelet lysate in combination with FBS was added. The results showed that 7% (v/v) platelet lysate was sufficient to supplement 10% (v/v) FBS in the culture of fibroblasts and keratinocytes. The combination of platelet lysate and FBS had a rather inhibitory effect on fibroblasts, in contrary to keratinocytes, where the effect was synergic. Platelet lysate did not sufficiently promote proliferation in melanocytes; however, the combination of FBS and platelet lysate yielded a better outcome and resulted in bipolar morphology of the cultured melanocytes. The data indicated that platelet lysate improved cell proliferation and metabolic activity and may be used as an additive to the cell culture media.</p