2,826 research outputs found
Dynamics of the kink effect in InAlAs/InGaAs/InP HEMTs
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1997.Includes bibliographical references (leaves 68-72).by Alexander N. Ernst.M.Eng
Temporal and spatial control of transgene expression using laser induction of the hsp70 promoter
BACKGROUND: Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that are difficult to characterize in non-model organisms. Here, we sought to eliminate the need for this type of sequence-based gene regulation and to open the field of functional genetics to a broader range of organisms. RESULTS: We have developed a new laser mediated method to heat shock groups of cells that provides precise spatio-temporal control of gene expression without requiring knowledge of specific enhancer sequences. We tested our laser-system in a transgenic line of Bicyclus anynana butterflies containing the EGFP reporter gene attached to the heat sensitive hsp70 promoter of Drosophila melanogaster. Whole organismal heat shocks demonstrated that this Drosophila promoter can drive gene expression in butterflies, and the subsequent laser heat shocks showed that it was possible to activate cell-specific gene expression in very precise patterns on developing pupal wings. CONCLUSION: This laser-mediated gene expression system will enable functional genetic investigations, i.e., the ectopic expression of genes and their knock-down in targeted groups of cells in model and non-model organisms with little or no available regulatory data, as long as a compatible heat-shock promoter is used and the target tissue is accessible to a laser beam. This technique will also be useful in evolutionary developmental biology as it will enable the study of the evolution of gene function across a variety of organisms
Physics of InAIAs/InGaAs Heterostructure Field-Effect Transistors
Contains an introduction, reports on three research projects, research conclusions and a list of publications.Joint Services Electronics Program Contract DAAHO4-95-1-003
Physics of InAIAs/InGaAs Heterostructure Field-Effect Transistors
Contains an introduction, a report on one research project and a list of publications and conference papers.Joint Services Electronics Program Contract DAAH04-95-1-0038Texas Instrument
Physics of Hemterostructure Field-Effect Transistors
Contains an introduction, report on one research project and a list of publications.Joint Services Electronics Program Contract DAAH04-95-1-0038Texas Instruments Agreement dated 11/23/9
Aluminum-, Calcium- And Titanium-Rich Oxide Stardust In Ordinary Chondrite Meteorites
We report isotopic data for a total of 96 presolar oxide grains found in
residues of several unequilibrated ordinary chondrite meteorites. Identified
grain types include Al2O3, MgAl2O4, hibonite (CaAl12O19) and Ti oxide. This
work greatly increases the presolar hibonite database, and is the first report
of presolar Ti oxide. O-isotopic compositions of the grains span previously
observed ranges and indicate an origin in red giant and asymptotic giant branch
(AGB) stars of low mass (<2.5 MSun) for most grains. Cool bottom processing in
the parent AGB stars is required to explain isotopic compositions of many
grains. Potassium-41 enrichments in hibonite grains are attributable to in situ
decay of now-extinct 41Ca. Inferred initial 41Ca/40Ca ratios are in good
agreement with model predictions for low-mass AGB star envelopes, provided that
ionization suppresses 41Ca decay. Stable Mg and Ca isotopic ratios of most of
the hibonite grains reflect primarily the initial compositions of the parent
stars and are generally consistent with expectations for Galactic chemical
evolution, but require some local interstellar chemical inhomogeneity. Very
high 17O/16O or 25Mg/24Mg ratios suggest an origin for some grains in binary
star systems where mass transfer from an evolved companion has altered the
parent star compositions. A supernova origin for the hitherto enigmatic
18O-rich Group 4 grains is strongly supported by multi-element isotopic data
for two grains. The Group 4 data are consistent with an origin in a single
supernova in which variable amounts of material from the deep 16O-rich interior
mixed with a unique end-member mixture of the outer layers. The Ti oxide grains
primarily formed in low-mass AGB stars. They are smaller and rarer than
presolar Al2O3, reflecting the lower abundance of Ti than Al in AGB envelopes.Comment: Accepted for publication in ApJ; 47 pages, 13 figure
Markov chain analysis of random walks on disordered medium
We study the dynamical exponents and for a particle diffusing
in a disordered medium (modeled by a percolation cluster), from the regime of
extreme disorder (i.e., when the percolation cluster is a fractal at )
to the Lorentz gas regime when the cluster has weak disorder at and
the leading behavior is standard diffusion. A new technique of relating the
velocity autocorrelation function and the return to the starting point
probability to the asymptotic spectral properties of the hopping transition
probability matrix of the diffusing particle is used, and the latter is
numerically analyzed using the Arnoldi-Saad algorithm. We also present evidence
for a new scaling relation for the second largest eigenvalue in terms of the
size of the cluster, , which provides a
very efficient and accurate method of extracting the spectral dimension
where .Comment: 34 pages, REVTEX 3.
Thermochronology of the Angara-Vitim granitoid batholith as a proxy indicator of plume events?
Dilepton production in proton-proton collisions at BEVALAC energies
The dilepton production in elementary reactions at
BEVALAC energies GeV is investigated. The calculations
include direct decays of the vector mesons , , and , Dalitz decays of the -, -, -, -, and -mesons, and of the baryon resonances
. The subthreshold vector meson production cross sections in
collisions are treated in a way sufficient to avoid double counting with the
inclusive vector meson production. The vector meson dominance model for the
transition form factors of the resonance Dalitz decays is
used in an extended form to ensure correct asymptotics which are in agreement
with the quark counting rules. Such a modification gives an unified and
consistent description of both radiative decays and meson decays.
The effect of multiple pion production on the experimental efficiency for the
detection of the dilepton pairs is studied. We find the dilepton yield in
reasonable agreement with the experimental data for the set of intermediate
energies whereas at the highest energy GeV the number of
dilepton pairs is likely to be overestimated experimentally in the mass range
MeV.Comment: 25 pages (IOP style), 5 figures, revised manuscript accepted for
publication in JP
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