Residual stress redistribution during elastic shake down in welded plates

Residual stresses are a consequence of welding in various structures such as ships and offshore structures. Residual stresses can be relaxed or redistributed according to the load levels during operation. The elastic shakedown phenomenon can be considered as one of the reasons for this change. This paper studies the relaxation/redistribution of weld residual stress during different levels of shakedown in a butt-welded plate chosen according to ship design and welding procedures. Welding was performed on DH36, a ship structural steel. Neutron diffraction was used to measure residual stresses in these plates in the as-welded state and after different levels of shakedown. A mixed hardening model in line with the Chaboche model is determined for both weld and base material. A numerical model is developed to estimate the shakedown limit on butt-welded plate. Further, the redistribution of residual stress in a numerical weld model according to the different levels of shakedown limit is studied. Based on the shakedown limit of the butt-welded plate, a shakedown region is determined, where the structure will undergo elastic shakedown in the presence of an existing residual stress field if the maximum stress on the load section after a few initial cycles is in the shakedown region

Genomic integration of transgenes using UV irradiation in Pristionchus pacificus

Transgenes are widely used throughout molecular biology for numerous applications. In Caenorhabditis elegans, stable transgenes are usually generated by microinjection into the germline establishing extrachromosomal arrays. Furthermore, numerous technologies exist to integrate transgenes into the C. elegans genome. In the nematode Pristionchus pacificus, transgenes are possible, however, their establishment is less efficient and dependent on the formation of complex arrays containing the transgene of interest and host carrier DNA. Additionally, genomic integration has only been reported via biolistic methods. Here we describe a simple technique using UV irradiation to facilitate the integration of transgenes into the P. pacificus genome

Manipulation monitoring and robot intervention in complex manipulation sequences

Compared to machines, humans are intelligent and dexterous; they are indispensable for many complex tasks in areas such as flexible manufacturing or scientific experimentation. However, they are also subject to fatigue and inattention, which may cause errors. This motivates automated monitoring systems that verify the correct execution of manipulation sequences. To be practical, such a monitoring system should not require laborious programming.Peer ReviewedPostprint (author's final draft