8 research outputs found
Π ΠΠΠ¬ EZH2 Π ARID1A Π ΠΠΠΠΠΠΠ‘Π’ΠΠΠ ΠΠΠΠ‘ΠΠΠ₯ Π£Π ΠΠ’ΠΠΠΠΠΠ¬ΠΠ«Π₯ ΠΠΠ£Π₯ΠΠΠΠ Π‘ ΠΠ’ΠΠΠΠΠ
Background. Diagnosis of urothelial carcinoma in situ is of great importance because it has prognostic and therapeutic value.We aim to determine the utility of EZH2 and ARID1A as a new tool in the diagnosis of carcinoma in situ.Material and Methods. This retrospective cross-sectional study included Twenty-four specimens of flat urothelial lesions, twenty specimens of CIS, and 10 of normal adjacent urothelium that was taken by cystoscopic resection biopsy procedure. immunohistochemical expression of EZH2 and ARID1A. were evaluated in all studied cases.Results. All normal urothelium specimens showed high nuclear staining for ARID1A and negative nuclear staining for EZH2. High EZH2 expression was observed in 80 % of CIS specimens compared to 20 % of flat urothelial lesions with atypia (p=0.001 ), while high ARID1A expression was observed in 70.8 % of flat urothelial lesions with atypia compared to 25 % of CIS specimens (p=0.001). EZH2 was more accurate and specific in the diagnosis of carcinoma in situ.Conclusion. EZH2 and ARID1A are promising diagnostic markers for urothelial CIS. EZH2 is more accurate and specific than ARID1A in the diagnosis of carcinoma in situ versus other flat urothelial lesions.Β ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ° ΡΡΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ in situ ΠΈΠΌΠ΅Π΅Ρ Π±ΠΎΠ»ΡΡΠΎΠ΅ Π·Π½Π°ΡΠ΅Π½ΠΈΠ΅, ΠΏΠΎΡΠΊΠΎΠ»ΡΠΊΡ ΠΎΠ±Π»Π°Π΄Π°Π΅Ρ ΠΏΡΠΎΠ³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΈ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠ΅Π½Π½ΠΎΡΡΡΡ.Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΠΈΡΡ ΡΠΎΠ»Ρ EZH2 ΠΈ ARID1A Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ in situ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π Π΅ΡΡΠΎΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ΅ ΠΏΠ΅ΡΠ΅ΠΊΡΠ΅ΡΡΠ½ΠΎΠ΅ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ Π²ΠΊΠ»ΡΡΠ°Π»ΠΎ 24 ΠΎΠ±ΡΠ°Π·ΡΠ° ΠΏΠ»ΠΎΡΠΊΠΈΡ
ΡΡΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ, 20 ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² CIS ΠΈ 10 ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΠΏΡΠΈΠ»Π΅Π³Π°ΡΡΠ΅Π³ΠΎ ΡΡΠΎΡΠ΅Π»ΠΈΡ, Π²Π·ΡΡΡΡ
ΠΏΡΠΈ ΡΠΈΡΡΠΎΡΠΊΠΎΠΏΠΈΡΠ΅ΡΠΊΠΎΠΉ Π±ΠΈΠΎΠΏΡΠΈΠΈ. ΠΠΎ Π²ΡΠ΅Ρ
ΡΠ»ΡΡΠ°ΡΡ
Π±ΡΠ»Π° ΠΎΡΠ΅Π½Π΅Π½Π° ΠΈΠΌΠΌΡΠ½ΠΎΠ³ΠΈΡΡΠΎΡ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ EZH2 ΠΈ ARID1A.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΠ΅ ΠΎΠ±ΡΠ°Π·ΡΡ Π½ΠΎΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ ΡΡΠΎΡΠ΅Π»ΠΈΡ ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΈ Π²ΡΡΠΎΠΊΠΎΠ΅ ΡΠ΄Π΅ΡΠ½ΠΎΠ΅ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ Π½Π° ARID1A ΠΈ ΠΎΡΡΠΈΡΠ°ΡΠ΅Π»ΡΠ½ΠΎΠ΅ ΡΠ΄Π΅ΡΠ½ΠΎΠ΅ ΠΎΠΊΡΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ Π½Π° EZH2. ΠΡΡΠΎΠΊΠ°Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ EZH2 Π½Π°Π±Π»ΡΠ΄Π°Π»Π°ΡΡ Π² 80 % ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² CIS ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ 20 % ΠΏΠ»ΠΎΡΠΊΠΈΡ
ΡΡΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ Ρ Π°ΡΠΈΠΏΠΈΠ΅ΠΉ (p=0,001), Π² ΡΠΎ Π²ΡΠ΅ΠΌΡ ΠΊΠ°ΠΊ Π²ΡΡΠΎΠΊΠ°Ρ ΡΠΊΡΠΏΡΠ΅ΡΡΠΈΡ ARID1A Π½Π°Π±Π»ΡΠ΄Π°Π»Π°ΡΡ Π² 70,8 % ΠΏΠ»ΠΎΡΠΊΠΈΡ
ΡΡΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ Ρ Π°ΡΠΈΠΏΠΈΠ΅ΠΉ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ 25 % ΠΎΠ±ΡΠ°Π·ΡΠΎΠ² CIS (Ρ=0,001). EZH2 Π±ΡΠ» Π±ΠΎΠ»Π΅Π΅ ΡΠΎΡΠ½ΡΠΌ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΡΠΌ ΠΏΡΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ in situ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. EZH2 ΠΈ ARID1A ΡΠ²Π»ΡΡΡΡΡ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΡΠΌΠΈ Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΠΌΠ°ΡΠΊΠ΅ΡΠ°ΠΌΠΈ ΡΡΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΠΎΠΉ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ in situ. EZH2 Π±ΠΎΠ»Π΅Π΅ ΡΠΎΡΠ΅Π½ ΠΈ ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ΅Π½, ΡΠ΅ΠΌ ARID1A, Π² Π΄ΠΈΠ°Π³Π½ΠΎΡΡΠΈΠΊΠ΅ ΠΊΠ°ΡΡΠΈΠ½ΠΎΠΌΡ in situ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π΄ΡΡΠ³ΠΈΠΌΠΈ ΠΏΠ»ΠΎΡΠΊΠΈΠΌΠΈ ΡΡΠΎΡΠ΅Π»ΠΈΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΎΠΏΡΡ
ΠΎΠ»ΡΠΌΠΈ.
Vitamin D level in preschool children with recurrent wheezy chest, and its relation to the severity of the wheezing episodes
Background: Recurrent wheezy chest is a common complaint in pediatric practice. Vitamin D is a potent immunomodulator in allergic diseases as wheezy chest and asthma. The prevalence of vitamin D deficiency has been increasing in Egypt leading to significant morbidities.Objectives: This study aimed to assess serum 25 hydroxy (OH) Vitamin D level in preschool children with recurrent wheezy chest, and to assess its relation to the recurrence, severity, and level of control of the wheezing episodes.Methods: The study included 100 preschool children (aged 2 to 5 years), of both sexes, recruited from the Emergency department, Allergy and Pulmonology units at Assiut University Children Hospital, Egypt. They should have at least 3 documented episodes of wheeze, cough, and difficulty breathing in the last year with clinical improvement on inhaled short-acting beta 2 agonists. Patients were subjected to questionnaire-based history, clinical examination, and laboratory investigations (complete blood count (CBC) with the absolute eosinophil count, serum total IgE level, and serum 25 hydroxy (OH) Vitamin D level). Pediatric Respiratory Assessment Measure (PRAM score) for assessment of the severity of the wheezing episodes and Global Initiative for Asthma (GINA) based level of asthma control for children 5 years and younger were applied. The patients were grouped according to PRAM score to mild, moderate and severe episodes and according to vitamin D level as sufficient and below-sufficient groups (including deficient and insufficient patients).Results: 25(OH) Vitamin D level was below-sufficient in 53% of the studied patients (deficient in 32% and insufficient in 21%). PRAM score was significantly lower in patients with sufficient 25(OH) Vitamin D level versus those with below-sufficient level (p < 0.025). There was a significant negative correlation between PRAM score and 25 (OH) Vitamin D level (r = -0.334, p = 0.001).Conclusion: There is an inverse relationship between 25(OH)vitamin D level and parameters of asthma severity, as well as with the level of asthma control in preschool children with recurrent wheezy chest.Keywords: Vitamin D, recurrent wheezy chest, preschool childre
FCM-Based Approach for Locating Visible Video Watermarks
The increased usage demand for digital multimedia has induced significant challenges regarding copyright protection, which is the copy control and proof of ownership. Digital watermarking serves as a solution to these kinds of problems. Among different types of digital watermarking, visible watermarking protects the copyrights effectively, since the approach not only prevents pirates but also visually proves the copyright of the broadcasted video. A visible watermark could be in any location on the frame (corner, center, diagonal, etc.). In addition, it could either completely or partially disappear for some frames. The same video also might have multiple watermarks. In order to strengthen the techniques of adding visible watermarks, there is a need to discover the weakness of the used watermarks. Since the major step of attacking a visible watermark is to locate it accurately, in this paper, a Fuzzy C-Means (FCM)-based approach is proposed to locate visible watermarks in video. Broadcasting channels are used to utilize video logos, which can be considered as a form of visible watermark that represents a trademark or symbol to declare the intellectual property ownership. In general, a high-standard video watermark has such properties as a clear background with distinctive shape without additional texture obscuring the watermark area. In addition, the probability of the logo appearing in the four corners of the video frames is higher than in the center. Based on these common properties of the video watermark, the proposed scheme locates the visible watermark using the Fuzzy C-Means technique without any prior information. The proposed technique has two stages: the first stage is positioning, and the second is masking (extracting the watermark mask). Due to real-world limitations such as noise, shadowing, and variations in cameras, the positioning stage is developed by employing gradient and Fuzzy C-Means classifier techniques. By using the dilation and erosion operators, the masking stage is developed to extract the watermark mask. Using a set of trademark videos, the proposed algorithm is tested and evaluated. A comparative study shows that the proposed FCM-based technique is able to achieve higher accuracy at a reasonable computational cost in comparison to the most related and recent published work. The proposed technique could locate different watermarks with high symmetry in their pattern, even if they appeared mutually in the same location. Still, it will be a challenge if the symmetry is low between used watermarks in the same location
Photobiomodulation and trigger band technique on groin adductor strain in athletes: Single-blinded randomized control trial
Aim of the study: In athletes, groin pain-related adductor strain is a common problem in sports medicine, like groin injuries, so the study investigated the effect of Photobiomodulation (905 nm) and Trigger Band Technique (TBT) on handball athletes' groin adductor strain. Methods: Forty handball athletes with adductor groin strain were divided into an experimental group (A) that received Diode laser 905nm, TBT, and medical treatment, while control group (B) received sham laser with TBT and medical treatment for four weeks. Outcome measures investigated Copenhangen hip and groin outcome score, pressure algometry, creatine kinase, and lactate dehydrogenase levels. Results: Both groups showed a significant improvement in the post-treatment outcome measures, where experimental group showed more significant improvement than the control group with p-value >0.05. Conclusion: results imply that treatment of handball athlete's groin adductor strain by TBT and low-level laser is more effective than TBT alone
Microbiome diversity in African American, European American, and Egyptian colorectal cancer patients
Purpose: Although there is an established role for microbiome dysbiosis in the pathobiology of colorectal cancer (CRC), CRC patients of various race/ethnicities demonstrate distinct clinical behaviors. Thus, we investigated microbiome dysbiosis in Egyptian, African American (AA), and European American (EA) CRC patients. Patients and methods: CRCs and their corresponding normal tissues from Egyptian (nΒ =Β 17) patients of the Alexandria University Hospital, Egypt, and tissues from AA (nΒ =Β 18) and EA (nΒ =Β 19) patients at the University of Alabama at Birmingham were collected. DNA was isolated from frozen tissues, and the microbiome composition was analyzed by 16S rRNA sequencing. Differential microbial abundance, diversity, and metabolic pathways were identified using linear discriminant analysis (LDA) effect size analyses. Additionally, we compared these profiles with our previously published microbiome data derived from Kenyan CRC patients. Results: Differential microbiome analysis of CRCs across all racial/ethnic groups showed dysbiosis. There were high abundances of Herbaspirillum and Staphylococcus in CRCs of Egyptians, Leptotrichia in CRCs of AAs, Flexspiria and Streptococcus in CRCs of EAs, and Akkermansia muciniphila and Prevotella nigrescens in CRCs of Kenyans (LDA score >4, adj. p-value <0.05). Functional analyses showed distinct microbial metabolic pathways in CRCs compared to normal tissues within the racial/ethnic groups. Egyptian CRCs, compared to normal tissues, showed lower l-methionine biosynthesis and higher galactose degradation pathways. Conclusions: Our findings showed altered mucosa-associated microbiome profiles of CRCs and their metabolic pathways across racial/ethnic groups. These findings provide a basis for future studies to link racial/ethnic microbiome differences with distinct clinical behaviors in CRC