Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference-0

<p><b>Copyright information:</b></p><p>Taken from "Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference"</p><p>http://www.biomedcentral.com/1472-6807/7/36</p><p>BMC Structural Biology 2007;7():36-36.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903359.</p><p></p>ht brown. Interactions responsible for sugar preference are in yellow, newly created hydrogen bonds are in blue. Figure clearly demonstrates different orientation of O6 of methyl mannoside

Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference-3

<p><b>Copyright information:</b></p><p>Taken from "Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference"</p><p>http://www.biomedcentral.com/1472-6807/7/36</p><p>BMC Structural Biology 2007;7():36-36.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903359.</p><p></p>uffer (pH = 7.5) with 30 μM CaCl. A) Data obtained from 29 automatic injections (10 μL) of Me-α-Man each into the S23A-containing cell. B)Plot of the total heat released as a function of ligand/protein molar ratio for the titration shown in panel A. The solid represents the best least-squares fit for the obtained data

Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference-4

<p><b>Copyright information:</b></p><p>Taken from "Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference"</p><p>http://www.biomedcentral.com/1472-6807/7/36</p><p>BMC Structural Biology 2007;7():36-36.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903359.</p><p></p>and PA-IIL wild type (red) by affinity chromatography on Mannose agarose column (HR 10/10). Loading buffer: 20 mM Tris/HCl, 100 mM NaCl, 100 μM CaCl, pH 7.5; Elution buffer: 20 mM Tris/HCl, 100 mM NaCl, 100 μM CaCl, 0.1 M D-mannose, pH 7.5; Sample: cytoplasmic soluble protein fractions