Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference-4

Abstract

<p><b>Copyright information:</b></p><p>Taken from "Engineering of PA-IIL lectin from – Unravelling the role of the specificity loop for sugar preference"</p><p>http://www.biomedcentral.com/1472-6807/7/36</p><p>BMC Structural Biology 2007;7():36-36.</p><p>Published online 1 Jun 2007</p><p>PMCID:PMC1903359.</p><p></p>and PA-IIL wild type (red) by affinity chromatography on Mannose agarose column (HR 10/10). Loading buffer: 20 mM Tris/HCl, 100 mM NaCl, 100 μM CaCl, pH 7.5; Elution buffer: 20 mM Tris/HCl, 100 mM NaCl, 100 μM CaCl, 0.1 M D-mannose, pH 7.5; Sample: cytoplasmic soluble protein fractions