22 research outputs found
The effect of Fertec-type liquid fertilizers on the ecological protection of the environment
In this scientific paper, we
have shown the apparent degrees of the
efficient use of nutrients from leaf fertilizers
(GAUPENICF) and the productive degrees of
the efficient use of nutrients from soil
reserves (GUPENsol), based on greenhouse
tomato yields, obtained after applying leaf
fertilizers. In our trials, we have tested the
fertilization method and leaf compositions
as methods of plant fertilization, for
correcting and supplementing crop nutrition
in greenhouses and preventing environment
pollution. By applying leaf fertilizers in
greenhouse tomato crops, we have obtained
high yield increases and a significant
diminution in the chemical pollution of the
environment. The incomplete use by the
crop of nutrients from applied fertilizers has
determined the increase in the incidence of
soil chemical pollution. However, soil
fertility may be diminished by high uptakes
of soil nutrients, under conditions of weak
nutrient supply of soil (without basic
fertilization). Therefore, we recommended
the concomitant use of soil and leaf
fertilization
Protein based capacitive biosensors: a new tool for structure-activity relationship studies
The present work reports a new application of a protein-based capacitive biosensor as an in vitro assay for the
selectivity study of the bacterial periplasmic protein MerP and four MerP variants. The modified MerP proteins were
produced by site-directed mutagenesis of the heavy metal associated motif (HMA). The MerP and modified MerPs
selectivity for copper, zinc, cadmium and mercury bivalent ions were investigated and compared. The variations in the
proteins affinity were related to the primary structure of the HMA motifs. Key amino acids for copper coordination of
metalloproteins that contain the metal binding sequence Gly-Met-Thr-Cys-xxx-xxx-Cys were identified. The results
brought insights valid for Menkes and Wilson ATPases. The protein-based capacitive biosensors were a simple and
useful tool for studying structure-activity relationships of proteins
Biosensors for life quality. Design, development and application
none10Biosensors, combining a selective biological recognition element and a sensitive transducer, are versatile analytical tools applied more
and more in different fields, such as medicine, food quality and safety control, and environment pollution monitoring. They are expected to
play an increasingly important role in the improvement of life quality. In this context, the present work covers recent approaches in design
and development of biosensors applied for analysis of real samples of medical, environmental or industrial relevance. The described sensors
meet the sensitivity, selectivity, and response time required by their applications. Moreover, they are designed to avoid contamination of the
monitored systems with undesirable components and to minimise the damage of living organisms (when it comes to in vivo monitoring).J. Castillo; S. Gáspár; S. Leth; M. Niculescu; A. Mortari; I. Bontidean; V. Soukharev; S.A. Dorneanu; A.D. Ryabov; E. CsöregiJ., Castillo; S., Gáspár; S., Leth; M., Niculescu; Mortari, Alessia; I., Bontidean; V., Soukharev; S. A., Dorneanu; A. D., Ryabov; E., Csöreg
Tuberculous Meningitis in Children and Adults: A 10-Year Retrospective Comparative Analysis.
Tuberculous meningitis (TBM) is the most lethal form of Mycobacterium tuberculosis infection, which has a high rate of neurological complications and sequelae.Our study offers a real-world infectious disease clinic perspective, being thus representative for the clinical environment of developing countries.We performed a retrospective analysis of the 127 adult and 77 pediatric cases diagnosed with TBM in the Infectious Disease Hospital of the School of Medicine of Iasi, Romania between 2004-2013.Definite diagnosis of TBM was established in 31% of children but in only 20% of adults (p = 0.043). A contact with an individual with pulmonary tuberculosis was documented in 30% of children vs. 13% of adults (p = 0.0007). Coma occurred in 19% of patients (similar in children and adults); other consciousness abnormalities were seen in 27% of children and in 72% of adults (p = 0.000001). Cranial nerve palsies occurred prior to therapy in 9% of cases (12% vs 7% of children and adults, respectively, p>0.05), and developed 2-7 days after treatment initiation in 10% (12 vs 9%). CSF cultures were positive for M. tuberculosis in 24% of patients (31% vs. 20%, p>0.05). Overall mortality was 7.35%, similar for children and adults. Yet, permanent neurological sequelae, which were seen in 23% of patients occurred significantly more frequent in children vs. adults (36% vs. 14%, respectively, p = 0.0121). In conclusion, our retrospective analysis on a significant number of cases of TBM identified striking differences between children and adults: while children were in an earlier stage at the admission, they associated a higher frequency of neurological sequelae and miliary pattern, and they were more likely to have normal CSF protein levels and positive cultures of CSF
Epidemiological aspects, case definition and stage of TBM on admission.
<p>Data are presented as n (%)</p><p>Epidemiological aspects, case definition and stage of TBM on admission.</p
Clinical features and infection outcome in patients with TBM.
<p>Data are presented as n (%)</p><p>Clinical features and infection outcome in patients with TBM.</p