Copper Nanostructured biohybrid material as broad-spectrum antiviral agent and its application as coating surface material

Respiratory pathogens kill more people than any other infectious agent each year worldwide. The development of novel economically-friendly, sustainable and highly efficient materials against viruses is a major challenge. Here we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized based on a new biohybrid technology that employs the use of a biological agent for its formation. This aqueous and room temperature stable material showed high virucidal activity against different viruses, including SARS-CoV-2. In addition, this new material has been successfully scaled-up and has been shown to have multiple applications as a coating agent on different surfaces of different composition (cotton, polyester, cellulose, paint, etc.). Interestingly, this nanomaterial showed antimicrobial-properties for the manufacture of antiviral face masks, maintaining high virucidal efficacy and stability, even after several washing cycles allowing its reuse.This work was supported by the Spanish National Research Council (CSIC)-Intramural Grant 202020E254, CSIC PTI Global Health (SGL2103036), and European Union (Nextgeneration EU). Authors thank Dr. Martinez from Novozymes for the gift of CALB enzyme.N

Nanostructured biohybrid material with wide-ranging antiviral action

Respiratory pathogens kill more people than any other infectious agent each year worldwide. Development of novel, economically friendly, sustainable, and highly efficient materials against viruses is a major challenge. Herein, we describe a nanostructured material composed of very small crystalline phosphate copper nanoparticles synthesized using a new biohybrid technology that employs a biological agent for its formation at room temperature in aqueous media. The evaluation of different enzymes in the final preparation of the nanomaterial or even in synthetic methods was performed. Biochemical characterization revealed the formation of Cu species in the protein network. The best biomaterial synthesized using a lipase called BioCuNPs showed excellent inhibition capacity against functional proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2); for example, assent 3-chymotrypsin like protease (3CLpro) complete inhibition was achieved by using 5 µg/mL, or acetone (ACE)–spike protein interaction was inhibited by more than 80% in the presence of 400 µg/mL of BioCuNPs. Taking these in vitro results into account, an efficacy analysis against human coronavirus 229E (HCoV-E229) coronavirus was performed. A virus reduction of 99% was obtained in 5 min. Additionally, SARS-CoV-2 virus was tested to demonstrate high efficiency, with > 99% inhibition in 15 min using 500 microgram of material. To determine the wide applicability of this nanohybrid against viruses, an evaluation was carried out against a non-enveloped virus such as Human Rhinovirus (HRV-14), obtaining a virus reduction of 99.9% in 5 min. Finally, the virucidal capacity against different bacteriophages was also evaluated, obtaining an excellent inhibition effect against Phage ϕX174 (99.999% reduction in 5 min).This work was supported by the Spanish National Research Council (CSIC) (projects Intramural Grant 202020E254), CSIC PTI-Global Health SGL2103036 (J. M. P), and European Union (Nextgeneration EU). Authors thank Dr. Martinez from Novozymes. This work was supported by Fundación hna (A. V-C. and O. A.). P. D-C. was financially supported by a Ramón y Cajal contract RYC2019-028015-I funded by MCIN/AEI.Peer reviewe

Vivir la calle

Capítulo de carácter antropológico dedicado a vivir la calle, enmarcado en un trabajo de investigación de recuperación de la memoria histórica del barrio valenciano del Cabanyal-Canyamela