Urban Agriculture and Ecosystem Services: A Typology and Toolkit for Planners

This thesis makes the connection between urban agriculture and a specific suite of ecosystem services and lays out a typology and toolkit for planners to take advantage of these ecosystem services. The services investigated here are: food production, water management, soil health, biodiversity, climate mitigation, and community development benefits. Research from a variety of fields was aggregated and synthesized to prove that urban agriculture can be beneficial for human as well as environmental health. A set of urban agriculture typologies was generated to illustrate best practices to maximize a particular set of ecosystem services. The typologies are: production farm, stormwater garden, soil-building garden, habitat garden, climate mitigation farm, cultural/educational garden, and ecosystem garden. Each typology was paired with a precedent study to demonstrate how that typology might be realized in the real world. Finally, a toolkit for planners was assembled to demonstrate some tools and techniques that planners might use to implement urban agriculture as a strategy for providing ecosystem services. Planners can utilize the toolkit to insert themselves into the urban ecosystem at multiple scales in a creative way to apply best practices and urban agriculture typologies in order to take advantage of the multiple benefits of urban agriculture

A closer look at ARSA activity in a patient with metachromatic leukodystrophy.

Metachromatic leukodystrophy (MLD) is an autosomal recessive lysosomal storage disease mainly caused by a deficiency of arylsulfatase A activity. The typical clinical course of patients with the late infantile form includes a regression in motor skills with progression to dysphagia, seizures, hypotonia and death. We present a case of a 4-year-old female with rapidly progressive developmental regression with loss of motor milestones, spasticity and dysphagia. MRI showed volume loss and markedly abnormal deep white matter. Enzymatic testing in one laboratory showed arylsulfatase A activity in their normal range. However, extraction of urine showed a large increase in sulfatide excretion in a second laboratory. Measurement of arylsulfatase A in that laboratory showed a partial decrease in arylsulfatase A activity measured under typical conditions (about 37% of the normal mean). When the concentration of substrate in the assay was lowered to one quarter of that normally used, this individual had activity \u3c10% of controls. The patient was found to be homozygous for an unusual missense mutation in the arylsulfatase A gene confirming the diagnosis of MLD. This case illustrates the importance of careful biochemical and molecular testing for MLD if there is suspicion of this diagnosis

Ethno-Religious Conflict In Northern Ireland

The Troubles, is an ethno-religious conflict within Northern Ireland that occurred from 1968 to 1998. During this conflict, the constitutional status of Northern Ireland was questioned—will it remain with the United Kingdom or finally become a united Ireland? Many scholars in political science discuss the roots of this conflict as an ethnic divide, citing anti-Irish discrimination and the perpetuation of ethnic stereotypes for centuries. However, scholars within this field fail to acknowledge the strong religious identities and anti-Catholicism. Without divulging and understanding each identities’ religious beliefs, scholars fail to understand how each identity sees not only themselves, but also others. I argue that the Troubles is not an ethno-national conflict, but an ethno-religious conflict that promoted two extreme forms of nationalism--the same pattern that has been present for centuries. The presence of paramilitary groups and discrimination within the political system divided these two groups even further. The Good Friday Agreement in 1998 led to the official end of the conflict, and promoted peace and stability in Northern Ireland. With the recent controversy of the United Kingdom leaving the European Union, the question of Northern Ireland’s constitutional status is back on the table

Novel Conversations, 1740-1817

“Novel Conversations” examines how and why eighteenth-century novelists came to represent people interacting in ways that registered as lively and real. Speech had long been crucial in literary genres as varied as drama, philosophical dialogue, romance and narrative poetry; but techniques for representing speech would proliferate in the eighteenth century as writers gave conversation a new centrality in the novel, seeking to capture the manner of speech over and above its basic matter. “Novel Conversations” explores this literary-historical development with chapters on four writers who were especially interested in the technical challenge of recording vocal effects: Samuel Richardson, James Boswell, Frances Burney and Jane Austen. They developed a set of tools for rendering in prose the auditory and social nuances of conversation, including tone and emphasis, pacing and pausing, gesture and movement. I argue that their experiments resulted in a new “transcriptional realism” in the novel. This term describes the range of techniques used to craft dialogue that faithfully approximates the features of real speech, while remaining meaningful and effectual as an element of prose narrative. In developing methods to this end, eighteenth-century writers borrowed techniques from other genres, combined them, and invented new ones. One rich source was life writing, the broad category of documentary prose genres that both absorbed and influenced the novel form in its early stages. Writers also sought complementary techniques in drama, whose stage directions, tonal notations and cues about who is speaking to whom at what point in time could be readily adapted for prose narrative. The task at hand was to calibrate two often opposing styles: the empirically driven, transcriptional mode of life writing and the more overtly stylized mode of drama. Writers did so by developing two resources within the novel form: the narrator, who occupies a flexible platform from which to elaborate conversational dynamics with description; and print itself, with all of its graphic and spatial possibilities for shaping speech on the page, including accidentals, line breaks, and typography. What are in one sense formalist readings are complemented by a careful attention to the materiality of the manuscript page and the printed page. In approaching my primary authors’ texts from a technical perspective, I do justice to their experimental efforts to use writing as a technology for capturing voice: a recording device avant la lettre. This approach in turn gives me critical purchase to analyze the effect that this technology serves: detailed representations of characters operating in a lively, familiar social world

Increasing the reliability of fully automated surveillance for central line–associated bloodstream infections

OBJECTIVETo increase reliability of the algorithm used in our fully automated electronic surveillance system by adding rules to better identify bloodstream infections secondary to other hospital-acquired infections.METHODSIntensive care unit (ICU) patients with positive blood cultures were reviewed. Central line–associated bloodstream infection (CLABSI) determinations were based on 2 sources: routine surveillance by infection preventionists, and fully automated surveillance. Discrepancies between the 2 sources were evaluated to determine root causes. Secondary infection sites were identified in most discrepant cases. New rules to identify secondary sites were added to the algorithm and applied to this ICU population and a non-ICU population. Sensitivity, specificity, predictive values, and kappa were calculated for the new models.RESULTSOf 643 positive ICU blood cultures reviewed, 68 (10.6%) were identified as central line–associated bloodstream infections by fully automated electronic surveillance, whereas 38 (5.9%) were confirmed by routine surveillance. New rules were tested to identify organisms as central line–associated bloodstream infections if they did not meet one, or a combination of, the following: (I) matching organisms (by genus and species) cultured from any other site; (II) any organisms cultured from sterile site; (III) any organisms cultured from skin/wound; (IV) any organisms cultured from respiratory tract. The best-fit model included new rules I and II when applied to positive blood cultures in an ICU population. However, they didn’t improve performance of the algorithm when applied to positive blood cultures in a non-ICU population.CONCLUSIONElectronic surveillance system algorithms may need adjustment for specific populations.Infect. Control Hosp. Epidemiol. 2015;36(12):1396–1400</jats:sec

What is translational research? Background, concepts, and a definition

Author version made available here in accordance with publisher copyright policy.This discussion paper aims to offer an overview and working definition of translational research, appropriate to health. Methods: Using scholarly and applied literature, the paper first identifies key challenges in achieving evidence-based policy and practice. It highlights international policy interest in new approaches to evidence translation and the barriers to achieving sound evidence translation. The paper offers an explicit definition of translational research and explains why it is important to have such a definition. It then elaborates on this definition by identifying and exploring seven distinctive research practices that could be associated with translational research. Findings and conclusions: Translational research is research with a sense of place. Its defining feature is excellence in evidence for a specific context or sphere of action, whether that is health policy for the World Health Organisation or service design for a local non-government organisation. If research is to be translated at all, it needs to be meaningful to many specific contexts, including small and regional contexts. The best promise that translational research offers is of exciting new techniques to achieve rigour and systemacy for such localised ‘real world’ policy, service and practice contexts

Estimating age-based antiretroviral therapy costs for HIV-infected children in resource-limited settings based on World Health Organization weight-based dosing recommendations

Background: Pediatric antiretroviral therapy (ART) has been shown to substantially reduce morbidity and mortality in HIV-infected infants and children. To accurately project program costs, analysts need accurate estimations of antiretroviral drug (ARV) costs for children. However, the costing of pediatric antiretroviral therapy is complicated by weight-based dosing recommendations which change as children grow. Methods: We developed a step-by-step methodology for estimating the cost of pediatric ARV regimens for children ages 0–13 years old. The costing approach incorporates weight-based dosing recommendations to provide estimated ARV doses throughout childhood development. Published unit drug costs are then used to calculate average monthly drug costs. We compared our derived monthly ARV costs to published estimates to assess the accuracy of our methodology. Results: The estimates of monthly ARV costs are provided for six commonly used first-line pediatric ARV regimens, considering three possible care scenarios. The costs derived in our analysis for children were fairly comparable to or slightly higher than available published ARV drug or regimen estimates. Conclusions: The methodology described here can be used to provide an accurate estimation of pediatric ARV regimen costs for cost-effectiveness analysts to project the optimum packages of care for HIV-infected children, as well as for program administrators and budget analysts who wish to assess the feasibility of increasing pediatric ART availability in constrained budget environments

Directed Trans-Differentiation of Thymus Cells into Parathyroid-Like Cells Without Genetic Manipulation

Replacement of a diseased organ with an autologously derived tissue is an ideal therapy for some medical problems. However, it is difficult to recreate many adult human tissues in vitro due to the functionally necessary architecture of most organs and the lack of understanding of methods to direct the development of the organ of interest. The parathyroid gland is ideal for in vitro organ development because this gland is relatively simple, is transplantable, and is commonly affected by a surgical complication rather than an autoimmune disease. We have investigated thymus as a source of autologous endoderm and parathyroid-like precursor cells. Human thymus cells were treated with a differentiation protocol we developed with human embryonic stem cells (The Bingham Protocol) that utilizes timed exposures to Activin A and soluble Sonic hedgehog (Shh). We incrementally changed the protocol to optimize the differentiation of the thymus cells into parathyroid-like cells. The final protocol used 50-ng/mL Activin A and 100-ng/mL Shh over 13 weeks. The differentiated cells expressed the parathyroid markers parathyroid hormone (PTH), calcium sensing receptor, chemokine receptor type-4 (CXCR4), and chorian-specific transcription factor (GCM2) as measured by reverse transcription-polymerase chain reaction and PTH enzyme-linked immunosorbent assay. Cultured thymus cells without Activin A or Shh exposure did not secrete PTH nor express similar markers. The differentiated cells released PTH, which was suppressed in response to increased calcium concentration. The chemically differentiated cells did not form tumors in immune-compromised mice. Our protocol recreated cells with markers of parathyroid tissue that responded as parathyroid cells to physiologic stimuli. This approach is a further step toward a strategy to restore parathyroid function using autologous cells that were directed to differentiate by nongenetic in vitro manipulation.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/90464/1/ten-2Etec-2E2011-2E0170.pd

Differentiation of Human Embryonic Stem Cells to a Parathyroid-Like Phenotype

Iatrogenic hypoparathyroidism is the most common complication of cervical endocrine surgery. Current management is limited and palliative. As the molecular steps in parathyroid development have been defined, they may be replicable in vitro, with a goal of cellular replacement therapy. Human embryonic stem cell (hESC) lines were investigated as a model for parathyroid regeneration in vitro. BG01 was selected as a model based on expression of genes of interest in embryoid bodies (EBs). Established strategies for mouse embryonic stem cell differentiation into definitive endoderm were modified and extended to maximize the expression of definitive markers of parathyroid development. The optimal approach included the use of Activin A at 100 ng/mL with BG01 cells grown on murine embryonic fibroblasts for 5 days under conditions of increasing serum concentration. After 5 days, the cells were allowed to mature further in tissue culture without murine fibroblasts but with continuous Activin A. Our strategy produced differentiated cell cultures that expressed intermediate markers of endoderm and parathyroid development (CXCR4, EYA1, Six1, and Pax1), as well as markers of committed parathyroid precursors or developed parathyroid glands (glial cell missing-2 [Gcm2], CCL21, calcium sensing receptor [CaSR], and parathyroid hormone [PTH]). We further characterized the cells by testing conditioned medium from various time points in our differentiation scheme for the presence of PTH. We found that by keeping the cells in culture 2 weeks after the withdrawal of Activin A, the cells were able to produce PTH. Further in vivo work will be needed to demonstrate proper functionality of the cells developed in this way.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/78132/1/scd.2008.0337.pd

Recreating Winchendon Village: A Distinct Destination in Toy Town (Winchendon, MA)

The goal of the Master of Regional Planning Studio is to develop a student’s techniques for collecting, analyzing, and synthesizing spatial and non-spatial data and then presenting that collective data in a manner (i.e., report, video, presentation, and charettes) that is understandable to academics, professionals, and the public. Planning Studio allows students to integrate knowledge from coursework and research, and apply such knowledge to resolving representative planning problems. At UMASS Amherst, these problems are found in neighborhood, rural, urban, and/or regional settings. In the fall of 2014, three local governments contracted with the MRP Studio to create separate vision plans that focused on key aspects of community revitalization. For the Town of Winchendon, the graduate student team (Carousel Consulting) created a revitalization plan that addressed declining investment and commercial activity in the Central Street corridor. As the “downtown” and commercial center of Winchendon, the strategy for Central Street focused on generating reinvestment through the advancement of commercial development and diversity, infill development and cohesion, place-making and branding, open space and recreation, and circulatory functionality