Uji Sitotoksisitas Ekstrak Ethanol 70 % Herba Ceplukan (Physalis Angulata Linn.) Terhadap Sel WiDr Secara in Vitro

Herba ceplukan (Physalis angulata Linn) merupakan salah satu bahan alam yang digunakanmasyarakat untuk pengobatan tradisional sebagai antikanker. Pada penelitian sebelumnya hasilpengujian ekstrak ethanol 70% herba ceplukan (Physalis angulata Linn.) terhadap Artemia salinaLeach (larva udang) dengan metode Brine Shirmp Lethality Test (BSLT) diketahui memiliki sifattoksik dengan nilai LC50 sebesar 39,63 μg/ml. Untuk itu dilakukan penelitian yang bertujuanmengetahui efek sitotoksisitas ekstrak ethanol 70% herba ceplukan dengan menentukan kadaryang menyebabkan 50% sel mati (LC50) terhadap sel kanker usus WiDr secara in vitro. Herbaceplukan (Physalis angulata Linn) diekstraksi dengan cara maserasi dengan penyari ethanol 70%.Sel kanker usus WiDr diperlakukan dengan ekstrak ethanol 70% herba ceplukan dengan seri kadaryaitu 1000, 500, 250, 125, 62.5, 31.25, 15.125 dan 7.81 μg/ml selama 24 jam. Sebagai kontrolpositif digunakan doksorubisin dengan seri kadar sebagai berikut: 2; 1; 0.5; 0.25; 0.12; 0.06; 0.03dan 0 .01 μg/ml. Uji sitotoksisitas dilakukan dengan menginkubasi sel kanker usus WiDr dengankepadatan akhir sel 2.104 sel / ml persumuran plat kultur. Uji sitotosisitas ini menggunakanmetode perhitungan langsung dengan bantuan alat haemocytometer. Hasil penelitian menunjukkanbahwa ekstrak ethanol 70% herba ceplukan (Physalis angulata Linn.) mempunyai sitotoksisitassebesar 86,84 ìg/ml terhadap sel kanker usus WiDr yang di atas nilai indikator positif sebagaibahan bersifat sitotoksik yaitu sebesar > 30 ìg/ml. Nilai tersebut juga sangat lebih tinggi biladibandingkan dengan nilai LC50 doksorubisin sebagai pembanding sebesar 0,113 μg/ml

Uji Aktivitas Antibakteri Ekstrak Air Bunga Kecombrang terhadap Bakteri E. Coli dan S. Aureus sebagai Bahan Pangan Fungsional

The testing has been conducted research on the antibacterial activity of aqueous extracts of flowers kecombrang against E. coli and S. Aureus. This study was aims to provided scientific evidence of excellence kecombrang plants as functional food ingredients. Antibacterial activity assays performed using dispersive method. From the results of antibacterial testing kecombrang flower water extract against E. coli concentration of 20% = 0 mm, 40% = 0 mm, 60% = 4.8 mm, 80% = 5.2 mm, 100% = 7.3 mm and the test bacteria S. aureus concentration of 20% = 8.67 mm, 40% = 9.11 mm, 60% = 12:33 mm, 80% = 12:44 mm, 100% = 13.89 mm

Kondisi Optimum Fusi Protoplas Antara Jamur Tiram Putih {Peurotus Floridae) Dan Jamur Tiram Coklat {Pleurotus Cystidiosusy[optimizing Conditions for Protoplast Fusion Between White Oyster Mushroom {Pleurotus Floridae) and Brown Oyster Mushroom {Pleurotus Cystidiosus)]

Genetic crossing of white oyster mushroom to introduce longer storage life trait can only be done within individuals in this particular species. However, longer storage life trait is possessed by brown oyster mushroom (Pleurotus cystidiosus) which is other species within this genus. Thefeore, protoplast fusion between white oyster mushroom (Peurotus floridae) and brown oyster mushroom (Pleurotus cystidiosus) was conducted to hopefully obtain an oyster mushroom strain that has higher production and longer storage life. Protoplast fusion was done by isolating protoplast from 5-days old monokaryotic mycelia grown in PDB. As much as 3.15 x 10 protoplasts/ml were harvested using mixture of cellulase Onozuka R-10 (1%) and macerozyme R-10 (1%) from brown oyster mushroom with 80.61% viability. Similarly, 3.71 x 10 protoplasts/ml were harvested using lysing enzyme (2%)from brown oyster mushroom with 83.68% viability. Protoplast fusion were conducted using 0% (control), 30%, 40% and 50% of PEG6000. Fusion periods were done at 10, 20 and 30 minutes. The candidate fusants were then screened using MRM (minimum regeneration media) media. Based on this experiment, the optimum conditions for protoplast fusion is 10 minutes incubation using 40% PEG6000 that yielded 121 colonies grown on MRM media as candidate fusants