Additional file 1 of Intracerebellar injection of monocytic immature myeloid cells prevents the adverse effects caused by stereotactic surgery in a model of cerebellar neurodegeneration

Additional file 1: Figure S1. Supplementary analyses on WT mice. A–C Charts showing behavioral and histological variables analyzed in the WT animals, comparing the control (untreated) and sham-operated mice. Note that surgery only increased the density of the apoptotic cells B but did not affect motor coordination A or the density of Purkinje cells in any of the lobes analyzed (C). C, D Immunofluorescence against calbindin (green) for staining Purkinje cells in the cerebellar sections of the control D and sham-operated E WT mice; note that surgery does not affect the general density of the neurons apart from the tissue damage at the injection site (arrowheads point to this region in E or its equivalent in untreated animals in D. *p < 0.05. Scale bar: 1 mm

Additional file 1: Table S1. of Tissue-type plasminogen activator exerts EGF-like chemokinetic effects on oligodendrocytes in white matter (re)myelination

Antibodies used in the study. Each antibody used in this study is listed together with its supplier, species, type (monoclonal/polyclonal), dilution and reference. (DOCX 16 kb

Additional file 3: Figure S2. of Tissue-type plasminogen activator exerts EGF-like chemokinetic effects on oligodendrocytes in white matter (re)myelination

EGFR is expressed in early oligodendrocyte precursors. Photomicrographs from embryonic spinal cord (ventral ventricular zone, E13) and perilesional adult corpus callosum (3dpi) WT mice tissue sections show representative confocal images of EGFR (green), Sox2 or PDGFRα (red) and DAPI (blue) immunoreactivities. Asterisks indicate PDGFRα+/EGFR− cells and arrowheads indicate PDGFRα+/EGFR+ cells (Representative images from n = 3). Scale bars: 10 μm. (TIF 585 kb

Additional file 4: Figure S3. of Tissue-type plasminogen activator exerts EGF-like chemokinetic effects on oligodendrocytes in white matter (re)myelination

tPA is not detected in PDFGR-β + pericytes. (A) Photomicrographs from adult WT mice tissue sections show representative confocal images of PDGFR-β (green), tPA (red) and DAPI (blue) immunoreactivities in the lesion 3 days after injection. (Representative images from n = 3). Inlets show side view reconstructions of the z-stack. (B) Representative fluorescent intensity/distance graph measured from confocal image in (A) showing that tPA (Red) is not found in colocalization with PDGFR-β staining. (TIF 328 kb