Home Sweet Home: The Impact of Lifestyle on a Cat’s Approach to Impossible Tasks in the Home Environment

: Cat welfare is a topic of growing interest in the scientific literature. Although previous studies have focused on the effects of living style (i.e., indoor/outdoor) on cat welfare, there has been a noticeable dearth of analysis regarding the impact of lifestyle on cats' inclination and mode of communication with humans. Our research aimed to analyze the possible effect of lifestyle (e.g., living indoors only or indoor/outdoor) on cat-human communication. The cats were tested using the impossible task paradigm test, which consists of some solvable trials in which the subject learns to obtain a reward from an apparatus, followed by an impossible trial through blocking the apparatus. This procedure triggers a violation of expectations and is considered a useful tool for assessing both the decision-making process and the tendency to engage in social behaviors towards humans. A specific ethogram was followed to record the behavioral responses of the cats during the unsolvable trial. Our results show the effects of lifestyle and age on domestic cats, providing valuable insights into the factors that influence their social behaviors. Cats that can roam freely outdoors spent less time interacting with the apparatus compared to indoor-only cats. Additionally, roaming cats showed stress behaviors sooner following the expectancy of violation compared to indoor cats. The lifestyle of cats can influence their problem-solving approach while not affecting their willingness to interact with humans or their overall welfare. Future studies on this topic can be useful for improving the welfare of domestic cats

Puppies in the problem-solving paradigm: quick males and social females

We report an observational, double-blind study that examined puppies’ behaviors while engaged in solving an experimental food retrieval task (food retrieval task instrument: FRTI). The experimental setting included passive social distractors (i.e., the dog’s owner and a stranger). The focus was on how the social and physical environment shapes puppies’ behaviors according to sex. The dependent variables were the number of tasks solved on an apparatus (Performance Index) and the time required to solve the frst task (Speed). Sex and Stress were set as explanatory factors, and Social Interest, FRTI interactions, other behavior, and age as covariates. The main fndings were that male puppies solved the frst task faster than females. On the other hand, females displayed signifcantly more social interest and did so more rapidly than males. Males showed delayed task resolution. This study demonstrates sex diferences in a problem-solving task in dog puppies for the frst time, thus highlighting that sexually dimorphic behavioral diferences in problem-solving strategies develop early on during ontogenesis.info:eu-repo/semantics/publishedVersio

Serum Oxytocin in Cows Is Positively Correlated with Caregiver Interactions in the Impossible Task Paradigm

SIMPLE SUMMARY: A possible relationship between circulating hormones (e.g., oxytocin and cortisol) and social behaviors toward humans in cows was studied using the impossible task paradigm test. Serum oxytocin levels correlated positively with the duration and negatively correlated with the latency of the cows’ social interactions with the caregiver. The implications of these findings for productivity as well as for animal welfare can be numerous and worth further investigation. ABSTRACT: This study explored a possible relationship between the circulating oxytocin, cortisol, and the willingness of dairy cows to engage in social behaviors with humans in an experimental context. The behaviors of twenty-nine cows were recorded during the impossible task paradigm, a procedure aimed at creating a violation of expectancy, in the presence of the caregiver and a stranger. The results showed that serum oxytocin levels were positively correlated with duration and negatively correlated with the latency of the cows’ social interactions with the caregiver. This research provides a clear correlation between circulating oxytocin and a willingness to engage in social contact with the caregiver, excluding the possible effect of different cortisol levels on such behavior

Effects of a Nutritional Supplement (DìRelaxTM) on Anxiety in Dogs in a Randomized Control Trial Design

SIMPLE SUMMARY: The effects of a nutraceutical product, DìRelax(TM), were tested in a cohort of anxious dogs by the C-BARQ questionnaire to assess the presence of problematic behaviors, and by the impossible task paradigm, an experimental procedure to explore dogs’ cognitive performance following an expectancy frustration. Hematological and biochemical analyses showed no adverse effects. The treatment with DìRelax(TM) showed a positive effect on the dog’s performances, with some of the behaviors appearing improved. The results suggested that DiRelax(TM) may have some ameliorative effect on the cognitive performances of anxious dogs. ABSTRACT: This study aimed to investigate the efficacy of DìRelax(TM), a nutraceutical formulated to reduce anxiety in dogs, using a randomized controlled trial (RCT) design. The C-BARQ questionnaire, some clinical investigations, and the impossible task test were performed in dogs before and after treatment. The C-BARQ questionnaire is particularly useful for assessing the frequency and severity of problematic behaviors. The impossible task paradigm provides insight into the decision-making processes in the realm of expectancy frustration. Results showed an ameliorative effect on the performances of treated dogs during the solvable phases, with a significant decrease in the time needed to solve the task. No behavioral difference was found between treated and untreated anxious dogs during the unsolvable phase. According to the results from the C-BARQ questionnaire, some of the behaviors appeared to improve. Clinical investigations, including a complete blood cell count and blood chemistry, showed no difference between groups, thus suggesting the safety of the product. In general, this study suggests that DìRelax(TM) can be safely administered with no adverse effects and can exercise a beneficial effect on anxious dogs by enhancing their cognitive abilities, but further studies should investigate the best method of administration

Phase 1/2 study of valproic acid and short-course radiotherapy plus capecitabine as preoperative treatment in low-moderate risk rectal cancer-V-shoRT-R3 (Valproic acid--short Radiotherapy--rectum 3rd trial).

BACKGROUND: Locally advanced rectal cancer (LARC) is a heterogeneous group of tumors where a risk-adapted therapeutic strategy is needed. Short-course radiotherapy (SCRT) is a more convenient option for LARC patients than preoperative long-course RT plus capecitabine. Histone-deacetylase inhibitors (HDACi) have shown activity in combination with RT and chemotherapy in the treatment of solid tumors. Valproic acid (VPA) is an anti-epileptic drug with HDACi and anticancer activity. In preclinical studies, our group showed that the addition of HDACi, including VPA, to capecitabine produces synergistic antitumour effects by up-regulating thymidine phosphorylase (TP), the key enzyme converting capecitabine to 5-FU, and by downregulating thymidylate synthase (TS), the 5-FU target. METHODS/DESIGN: Two parallel phase-1 studies will assess the safety of preoperative SCRT (5 fractions each of 5 Gy, on days 1 to 5) combined with (a) capecitabine alone (increasing dose levels: 500-825 mg/m2/bid), on days 1-21, or (b) capecitabine as above plus VPA (oral daily day -14 to 21, with an intra-patient titration for a target serum level of 50-100 microg/ml) followed by surgery 8 weeks after the end of SCRT, in low-moderate risk RC patients. Also, a randomized phase-2 study will be performed to explore whether the addition of VPA and/or capecitabine to preoperative SCRT might increase pathologic complete tumor regression (TRG1) rate. A sample size of 86 patients (21-22/arm) was calculated under the hypothesis that the addition of capecitabine or VPA to SCRT can improve the TRG1 rate from 5% to 20%, with one-sided alpha = 0.10 and 80% power.Several biomarkers will be evaluated comparing normal mucosa with tumor (TP, TS, VEGF, RAD51, XRCC1, Histones/proteins acetylation, HDAC isoforms) and on blood samples (polymorphisms of DPD, TS, XRCC1, GSTP1, RAD51 and XRCC3, circulating endothelial and progenitors cells; PBMCs-Histones/proteins acetylation). Tumor metabolism will be measured by 18FDG-PET at baseline and 15 days after the beginning of SCRT. DISCUSSION: This project aims to improve the efficacy of preoperative treatment of LARC and to decrease the inconvenience and the cost of standard long-course RT. Correlative studies could identify both prognostic and predictive biomarkers and could add new insight in the mechanism of interaction between VPA, capecitabine and RT.EudraCT Number: 2012-002831-28. TRIAL REGISTRATION: ClinicalTrials.gov number, NCT01898104

18F-FDG PET/CT Is an Early Predictor of Pathologic Tumor Response and Survival to Preoperative Radiochemotherapy with Bevacizumab in High Risk Locally Advanced Rectal Cancer.

There is an unmet need for predictive biomarkers of the clinical benefit of anti-angiogenic drugs.The aim of the present study was to prospectively evaluate the value of FDG-PET/CT performed during and after preoperative radiochemotherapy (CRT) with bevacizumab for the prediction of complete pathologic tumor regression and survival in magnetic resonance imaging (MRI)-defined high-risk locally advanced rectal cancer (LARC) patients Methods: Sixty-one patients treated in a non-randomized phase II study (BRANCH) with concomitant or sequential (4 days before CRT) administration of bevacizumab with preoperative CRT were included. 18F-FDG PET/CT was performed at baseline, 11 days after the beginning of the CRT (early) and before surgery (late). Metabolic changes were compared with pathological complete (TRG1) vs incomplete (TRG2-5) tumor regression, progression-free survival (PFS), cancer specific survival (CSS) and overall survival (OS). Receiver operating characteristic (ROC) curves were calculated for those FDG-PET/CT parameters that significantly correlated with TRG1. Results: Early total lesion glycolysis (TLG-early) and its percent change compared to baseline (ΔTLG-early %) could discriminate TRG1 from TRG2-5. Only ROC analysis of ΔTLG-early % showed an AUC > 0.7 (0.76), with an optimal cutoff at 59.5% (80% sensitivity, 71.4 specificity) for identifying TRG1. Late metabolic assessment could not discriminate between the two groups. After a median follow-up of 98 months (range 77-132) metabolic responders (ΔTLG-early % ≥ 59.5 %) demonstrated a significantly higher 10-year PFS (89.3 vs 63.6 %, P = 0.02) and CSS (92.9 vs 72.6 %, P = 0.04) compared to incomplete metabolic responders . Conclusion: Our results suggest that early metabolic response can act as a surrogate marker of the benefit of antiangiogenic therapy and provide further support for the use of early -FDG-PET/CT evaluation to predict pathological response and survival in the preoperative treatment of patients with LARC. ΔTLG-early% showed the best accuracy in predicting TRG and may be particularly useful in guiding treatment modifying decisions during preoperative CRT based on expected response

When are puppies receptive to emotion-induced human chemosignals? The cases of fear and happiness

We report an observational, double-blind, experimental study that examines the efects of human emotional odors on puppies between 3 and 6 months and adult dogs (one year and upwards). Both groups were exposed to control, human fear, and happiness odors in a between subjects’ design. The duration of all behaviors directed to the apparatus, the door, the owner, a stranger, and stress behaviors was recorded. A discriminant analysis showed that the fear odor activates consistent behavior patterns for both puppies and adult dogs. However, no behavioral diferences between the control and happiness odor conditions were found in the case of puppies. In contrast, adult dogs reveal distinctive patterns for all three odor conditions. We argue that responses to human fear chemosignals systematically infuence the behaviors displayed by puppies and adult dogs, which could be genetically prefgured. In contrast, the efects of happiness odors constitute cues that require learning during early socialization processes, which yield consistent patterns only in adulthood.info:eu-repo/semantics/publishedVersio

Puppies in the problem-solving paradigm: quick males and social females

We report an observational, double-blind study that examined puppies' behaviors while engaged in solving an experimental food retrieval task (food retrieval task instrument: FRTI). The experimental setting included passive social distractors (i.e., the dog's owner and a stranger). The focus was on how the social and physical environment shapes puppies' behaviors according to sex. The dependent variables were the number of tasks solved on an apparatus (Performance Index) and the time required to solve the first task (Speed). Sex and Stress were set as explanatory factors, and Social Interest, FRTI interactions, other behavior, and age as covariates. The main findings were that male puppies solved the first task faster than females. On the other hand, females displayed significantly more social interest and did so more rapidly than males. Males showed delayed task resolution. This study demonstrates sex differences in a problem-solving task in dog puppies for the first time, thus highlighting that sexually dimorphic behavioral differences in problem-solving strategies develop early on during ontogenesis