3,765 research outputs found
EVALITA 2020: Overview of the 7th evaluation campaign of natural language processing and speech tools for Italian
The Evaluation Campaign of Natural Language Processing and Speech Tools for Italian (EVALITA) is the biennial initiative aimed at promoting the development of language and speech technologies for the Italian language. EVALITA is promoted by the Italian Association of Computational Linguistics (AILC) and it is endorsed by the Italian Association for Artificial Intelligence (AIxIA) and the Italian Association for Speech Sciences (AISV).
EVALITA provides a shared framework where different systems and approaches can be scientifically evaluated and compared with each other with respect to a large variety of tasks, suggested and organized by the Italian research community. The proposed tasks represent scientific challenges where methods, resources, and systems can be tested against shared benchmarks representing linguistic open issues or real world applications, possibly in a multilingual and/or multi-modal perspective. The collected data
sets provide big opportunities for scientists to explore old and new problems concerning NLP in Italian as well as to develop solutions and to discuss the NLP-related issues within the community. Some tasks are traditionally present in the evaluation campaign, while others are completely new.
This paper introduces the tasks proposed at EVALITA 2020 and provides an overview to the participants and systems whose descriptions and obtained results are reported in these Proceedings
Trans-generational epigenetic regulation associated with the amelioration of Duchenne Muscular Dystrophy
Exon skipping is an effective strategy for the treatment of many Duchenne Muscular Dystrophy (DMD) mutations. Natural exon skipping observed in several DMD cases can help in identifying novel therapeutic tools. Here, we show a DMD study case where the lack of a splicing factor (Celf2a), which results in exon skipping and dystrophin rescue, is due to a maternally inherited trans-generational epigenetic silencing. We found that the study case and his mother express a repressive long non-coding RNA, DUXAP8, whose presence correlates with silencing of the Celf2a coding region. We also demonstrate that DUXAP8 expression is lost upon cell reprogramming and that, upon induction of iPSCs into myoblasts, Celf2a expression is recovered leading to the loss of exon skipping and loss of dystrophin synthesis. Finally, CRISPR/Cas9 inactivation of the splicing factor Celf2a was proven to ameliorate the pathological state in other DMD backgrounds establishing Celf2a ablation or inactivation as a novel therapeutic approach for the treatment of Duchenne Muscular Dystrophy
Genome-wide activity of unliganded estrogen receptor-\u3b1\ua0 in breast cancer cells
Estrogen receptor-\u3b1 (ER\u3b1) has central role in hormone-dependent
breast cancer and its ligand-induced functions have been extensively
characterized. However, evidence exists that ER\u3b1 has functions that
are independent of ligands. In the present work, we investigated the
binding of ER\u3b1 to chromatin in the absence of ligands and its functions
on gene regulation. We demonstrated that in MCF7 breast cancer
cells unliganded ER\u3b1 binds to more than 4,000 chromatin sites.
Unexpectedly, although almost entirely comprised in the larger group
of estrogen-induced binding sites, we found that unliganded-ER\u3b1
binding is specifically linked to genes with developmental functions,
compared with estrogen-induced binding. Moreover, we found that
siRNA-mediated down-regulation of ER\u3b1 in absence of estrogen is
accompanied by changes in the expression levels of hundreds of
coding and noncoding RNAs. Down-regulatedmRNAs showed enrichment
in genes related to epithelial cell growth and development.
Stable ER\u3b1 down-regulation using shRNA, which caused cell growth
arrest, was accompanied by increased H3K27me3 at ER\u3b1 binding
sites. Finally, we found that FOXA1 and AP2\u3b3 binding to several sites
is decreased upon ER\u3b1 silencing, suggesting that unliganded ER\u3b1
participates, together with other factors, in the maintenance of the
luminal-specific cistrome in breast cancer cell
Genome-wide association study provides insights into genes related with horn development in Nelore beef cattle.
Abstract The causal mutation for polledness in Nelore (Bos taurus indicus) breed seems to have appeared first in Brazil in 1957. The expression of the polled trait is known to be ruled by a few groups of alleles in taurine breeds; however, the genetic basis of this trait in indicine cattle is still unclear. The aim of this study was to identify genomic regions associated with the hornless trait in a commercial Nelore population. A total of 107,294 animals had phenotypes recorded and 2,238 were genotyped/imputed for 777k SNP. The weighted single-step approach for genome-wide association study (WssGWAS) was used to estimate the SNP effects and variances accounted for by 1 Mb sliding SNP windows. A centromeric region of chromosome 1 with 3.11 Mb size (BTA1: 878,631?3,987,104 bp) was found to be associated with hornless in the studied population. A total of 28 protein-coding genes are mapped in this region, including the taurine Polled locus and the IFNAR1, IFNAR2, IFNGR2, KRTAP11-1, MIS18A, OLIG1, OLIG2, and SOD1 genes, which expression can be related to the horn formation as described in literature. The functional enrichment analysis by DAVID tool revealed cytokine-cytokine receptor interaction, JAK-STAT signaling, natural killer cell mediated cytotoxicity, and osteoclast differentiation pathways as significant (P < 0.05). In addition, a runs of homozygosity (ROH) analysis identified a ROH island in polled animals with 2.47 Mb inside the region identified by WssGWAS. Polledness in Nelore cattle is associated with one region in the genome with 3.1 Mb size in chromosome 1. Several genes are harbored in this region, and they may act together in the determination of the polled/horned phenotype. Fine mapping the locus responsible for polled trait in Nelore breed and the identification of the molecular mechanisms regulating the horn growth deserve further investigation
3D structure of individual mammalian genomes studied by single cell Hi-C
The folding of genomic DNA from the beads-on-a-string like structure of nucleosomes into higher order assemblies is critically linked to nuclear processes. We have calculated the first 3D structures of entire mammalian genomes using data from a new chromosome conformation capture procedure that allows us to first image and then process single cells. This has allowed us to study genome folding down to a scale of <100 kb and to validate the structures. We show that the structures of individual topological-associated domains and loops vary very substantially from cell-to-cell. By contrast, A/B compartments, lamin-associated domains and active enhancers/promoters are organized in a consistent way on a genome-wide basis in every cell, suggesting that they could drive chromosome and genome folding. Through studying pluripotency factor- and NuRD-regulated genes, we illustrate how single cell genome structure determination provides a novel approach for investigating biological processes.We thank the Wellcome Trust (082010/Z/07/Z), the EC FP7 4DCellFate project (277899) and the MRC (MR/M010082/1) for financial support
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Search for MSSM Higgs bosons decaying to μ+μ-in proton-proton collisions at √s=13TeV
A search is performed for neutral non-standard-model Higgs bosons decaying to two muons in the context of the minimal supersymmetric standard model (MSSM). Proton-proton collision data recorded by the CMS experiment at the CERN Large Hadron Collider at a center-of-mass energy of 13TeVwere used, corresponding to an integrated luminosity of 35.9fb-1. The search is sensitive to neutral Higgs bosons produced via the gluon fusion process or in association with a bbquark pair. No significant deviations from the standard model expectation are observed. Upper limits at 95% confidence level are set in the context of the mmod+hand phenomenological MSSM scenarios on the parameter tanβas a function of the mass of the pseudoscalar Aboson, in the range from 130 to 600GeV. The results are also used to set a model-independent limit on the product of the branching fraction for the decay into a muon pair and the cross section for the production of a scalar neutral boson, either via gluon fusion, or in association with bquarks, in the mass range from 130 to 1000GeV
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Studies of Bs2∗(5840)0 and Bs1(5830)0 mesons including the observation of the Bs2∗(5840)0→B0KS0 decay in proton-proton collisions at s=8TeV.
Measurements of Bs2∗(5840)0 and Bs1(5830)0 mesons are performed using a data sample of proton-proton collisions corresponding to an integrated luminosity of , collected with the CMS detector at the LHC at a centre-of-mass energy of 8TeV . The analysis studies P-wave Bs0 meson decays into B(∗)+K- and B(∗)0KS0 , where the B+ and B0 mesons are identified using the decays B+→J/ψK+ and B0→J/ψK∗(892)0 . The masses of the P-wave Bs0 meson states are measured and the natural width of the Bs2∗(5840)0 state is determined. The first measurement of the mass difference between the charged and neutral B∗ mesons is also presented. The Bs2∗(5840)0 decay to B0KS0 is observed, together with a measurement of its branching fraction relative to the Bs2∗(5840)0→B+K- decay
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