Campylobacter cuniculorum sp nov., from rabbits

Eight strains of an unknown thermotolerant Campylobacter species were isolated from the caecal contents of rabbits (Oryctolagus cuniculus). All strains were initially identified as belonging to the genus Campylobacter by means of genus-specific PCR, but none were identified using species-specific PCR for known thermophilic species. Cells were spiral shaped with bipolar unsheathed flagella, with no periplasmic fibres, and appeared coccoid after 10-12 days of incubation. Phylogenetic analyses based on 16S rRNA gene, rpoB and groEL sequences revealed that all strains formed a robust clade that was very distinct from recognized Campylobacter species. 16S rRNA gene sequence pairwise comparisons of strain 150B(T) with the type strains of other Campylobacter species revealed that the nearest phylogenetic neighbour was Campylobacter helveticus NCTC 12470(T), with 96.6% similarity. The uniqueness of these rabbit isolates was confirmed by whole-cell protein electrophoresis. Taken together, these data indicate that the strains belong to a novel Campylobacter species for which the name Campylobacter cuniculorum sp. nov. is proposed, with 150B(T) (=LMG 24588(T) =CCUG 56289(T)) as the type strain

Reflection on the development process of a sustainability assessment tool : learning from a Flemish case

Adoption of sustainability assessment tools in agricultural practice is often disappointing. One of the critical success factors for adoption is the tool development process. Because scientific attention to these development processes and insights about them are rather limited, we aimed to foster the scientific debate on this topic. This was done by reflecting on the development process of a Flemish sustainability assessment tool, MOTIFS. MOTIFS was developed with the aim of becoming widely adopted by farmers and farm advisors, but this result was not achieved. Our reflection process showed success factors favoring and barriers hindering tool adoption. These were grouped into three clusters of lessons learned for sound tool development: (1) institutional embeddedness, (2) ownership, and (3) tool functions. This clustering allowed us to formulate actions for researchers on the following aspects: (1) learning from stakeholders and end users, (2) providing coaching for appropriate tool use, and (3) structuring development of different tool types and exploring spin-offs from existing tools. We hope these normative results evoke other researchers to feed a debate on understanding tool development

A reflexive assessment of a regional initiative in the agri-food system to test whether and how it meets the premises of transdisciplinary research

In recent sustainability assessments, transdisciplinary approaches have been used to bridge contested normative views among many societal actors, policymakers and researchers. Transdisciplinary research is mainly based on three premises: (1) various perspectives need to be incorporated and discussed to empower actors, (2) the collaboration and co-creation of knowledge between academic and societal actors allows to better grasp the complexity of a real-world problem, and (3) a mutual learning process arises which could increase the legitimacy of decisions and their implementation in practice. Despite many examples of transdisciplinary projects, few assessments have been done that question whether such a project fulfils the premises of transdisciplinary research. Therefore, we report on a reflexive assessment of an initiative set up to foster the transformation of the Flemish agri-food system towards sustainability. The case study involved both scientific and societal actors with different views, making it possible to implement a transdisciplinary process, evaluate the expectations and give suggestions for future processes. Evaluation criteria are linked to context, process and outcomes. Analysis of the qualitative and quantitative data indicates that the initiative did empower the transformation towards sustainability. Furthermore, results show that, in this case, a transdisciplinary approach can fulfil its premises. However, some critical factors are identified, such as the importance of the context specificity and a flexible, adaptive and iterative process

Campylobacter insulaenigrae: first isolation report from South American sea lion (Otaria flavescens, (Shaw, 1800)

Campylobacter insulaenigrae have been isolated from different pinnipeds but not from South American sea lion (Otaria flavescens). The aim of this work is to report the first isolation of C. insulaenigrae from South American sea lion (Otaria flavescens). The isolate, identified by its phenotypic and molecular characteristics, allow recognizing O. flavescens as a new host for C. insulaenigrae

Key characteristics for tool choice in indicator-based sustainability assessment at farm level

Although the literature on sustainability assessment tools to support decision making in agriculture is rapidly growing, little attention has been paid to the actual tool choice. We focused on the choice of more complex integrated indicator-based tools at the farm level. The objective was to determine key characteristics as criteria for tool choice. This was done with an in-depth comparison of 2 cases: the Monitoring Tool for Integrated Farm Sustainability and the Public Goods Tool. They differ in characteristics that may influence tool choice: data, time, and budgetary requirements. With an enhanced framework, we derived 11 key characteristics to describe differences between the case tools. Based on the key characteristics, we defined 2 types of indicator-based tools: full sustainability assessment (FSA) and rapid sustainability assessment (RSA). RSA tools are more oriented toward communicating and learning. They are therefore more suitable for use by a larger group of farmers, can help to raise awareness, trigger farmers to become interested in sustainable farming, and highlight areas of good or bad performance. If and when farmers increase their commitment to on-farm sustainability, they can gain additional insight by using an FSA tool. Based on complementary and modular use of the tools, practical recommendations for the different end users, i.e., researchers, farmers, advisers, and so forth, have been suggested

Structural aspects of on-farm demonstrations: key considerations in the planning and design process

Research on the adoption and diffusion of innovations has consistently confirmed that one of farmers’ most commonly cited sources of information and ideas is other farmers. Demonstrations concern the practical exhibition (and explanation) of how something works and have for long been one of the most important extension techniques. On-farm demonstrations facilitate an effective learning situation for farmers to “See the crops themselves”, “interact with the scientists and extension workers on the field”, and “get doubts clarified themselves”. In the literature concerning on-farm demonstrations, a wide range of structural characteristics are described. These differ according to the actors/networks involved and their roles, the audience/ attendees, the network structure and its characteristics, resources, finances and incentives, and characteristics related to the farm (geographic location, accessibility, etc.). The current paper will elaborate on such structural characteristics and will thus contribute to building a provisional model of the linkages between rationale (goals, objectives, topics), structural factors, functions and results (output, outcomes and impact)